Summary
Effect of some negative stains used in electron microscopy on calcium-transporting membranes of the fragmented sarcoplasmic reticulum (FSR) and interaction between stains and calcium have been studied.
Calcium transport as well as ATP-ase activity of FSR are affected by stains investigated at concentrations lower than those used in electron microscopy. Degree of alteration varies from stain to stain. Phosphotungstic acid is the most effective inhibitor.
Calcium loaded vesicles are depleted during resuspension in various staining solutions, in concentration routinely used for electron microscopy, according to the calcium affinity of the stain. Also in this respect phosphotungstic acid is the most effective.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agostini, B.: Localisation of product of ATP-ase activity and negative staining of mitochondria. J. Submicr. Cytol. 1, 283 (1969) (Abstract).
Bray, G. A.: A simple efficient liquid scintillator for counting aqueous solution in a liquid scintillation counter. Anal. Biochem. 1, 279–285 (1960).
Chimoskey, J. E., Gergely, J.: Effect of ions on sarcoplasmatic reticulum fragments. Arch. Biochem. Biophys. 128, 601–605 (1968).
Deamer, D. W., Baskin, R. J.: Ultrastructure of sarcoplasmic reticulum preparations. J. Cell Biol. 42, 296–307 (1969).
Ebashi, S., Lipmann, F.: Adenosine triphosphate-linked concentration of calcium ions in a particulate fraction of rabbit muscle. J. Cell Biol. 14, 389–400 (1962).
Gmelin: Handbuch der anorganischen Chemie, Bd. 53, Molybdän, S. 291–293; Bd. 54, Wolfram, S. 289–295 u. 358–375; Bd. 55, Uran, S. 230–231. (Dtsch. Chem. Ges., R. J. Meyer) Berlin: Verlag Chemie 1935.
Hasselbach, W.: Relaxing factor and the relaxation of muscle. Progr. Biophys. molec. Biol. 14, 167–222 (1964).
—, Makinose, M.: Die Calciumpumpe der „Erschlaffungsgrana“ des Muskels und ihre Ab-hängigkeit von der ATP-Spaltung. Biochem. Z. 333, 518–528 (1961).
—: Über den Mechanismus des Calciumtransportes durch die Membranen des sarkoplasmatischen Retikulums. Biochem. Z. 339, 94–111 (1963).
Hörne, R. W.: Negative staining methods. In: Techniques for electron microscopy (D. H. Kay ed.), p. 328–355. Oxford: Blackwell Sci. Publ. 1965.
Martonosi, A., Feretos, R.: Sarcoplasmic reticulum. I. The uptake of Ca++ by sarcoplasmic reticulum fragments. J. Biol. Chem. 239, 648–658 (1964).
Muscatello, U., Guarriero-Bobyleva, V.: Effect of negative stains used in electron microscopy on some biochemical parameters of mitochondrial activity. J. Ultrastruct. Res. 31, 337–348 (1970).
Rockstein, M., Herron, P. W.: Colorimetric determination of inorganic phosphate in microgram quantities. Analyt. Chem. 23, 1500–1501 (1951).
Sommer, J. R., Hasselbach, W.: The effect of glutaraldehyde and formaldehyde on the calcium pump of the sarcoplasmic reticulum. J. Cell Biol. 34, 902–905 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Agostini, B., Hasselbach, W. Effect of some negative stains on the calcium transport of sarcoplasmic reticulum. Histochemie 27, 303–309 (1971). https://doi.org/10.1007/BF00305332
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00305332