Summary
The histochemical value of fractionated histochromatographic examination of lipids according to Holczabek's (1969) method which gives very good results as regards chromatography, is limited by that phospholipids are not retained quantitatively in sites of their original localization in sections during the first developing in a mixture of petrolether:ether:acetic acid. The staining of phospholipids performed after the first developing therefore does not reveal the localization pattern of all phospholipids.
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Šmíd, F., Lojda, Z. Studies in lipid histochemistry. Histochemie 27, 300–302 (1971). https://doi.org/10.1007/BF00305331
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DOI: https://doi.org/10.1007/BF00305331