Anatomy and Embryology

, Volume 177, Issue 6, pp 543–556 | Cite as

Morphological study of cerebellar transplant cocultivated with cerebral cortical graft in the anterior eye chamber

I. Granular layer
  • J. Takács
  • J. Hámori


Fetal cerebral cortex and cerebellar anlage from rat fetuses of 15–16 gestational day were grafted simultaneously to the anterior eye chamber of adult female albino rat recipients. Two months after transplantation the cerebellar portion of the double graft consisted of foliated cerebellar cortex surrounding a welldefined cerebellar nucleus. In the absence of pia mater or glial scar the cerebral and cerebellar grafts were observed to establish direct contact with each other.

Although much thinner than in the normal cerebellum, the overall morphological organization of the granular layer in the transplant was similar to that described for “in situ” normal cerebellum, with some remarkable differences, though. In normal cerebellum all mossy terminals contain spheroid synaptic vesicles, a characteristic morphological feature of excitatory endings. In the transplant, however, although the majority of mossy terminals contained (small or large) spheroid synaptic vesicles, numerous mossy terminals were filled with ovoid, or pleomorphic synaptic vesciles, a morphological marker of inhibitory terminals. GABA-immunogold reaction, revealed, indeed, the presence of this inhibitory transmitter in mossy terminals containing ovoid synaptic vesicles. Both GABA (-) and GABA (+) mossy terminals formed asymmetric (Gray I-type) synaptic junctions with the surrounding dendritic digits of granule cells. It is suggested that GABA-ergic fibers as well as most non-GABA-ergic axons (originating either from the cerebral cortical graft, or from the cerebellar nucleus) may develop to mossy terminal-like structures as a consequence of the huge deficit in “natural” mossy fibers in this model.

Key words

Cerebro-cerebellar transplant Glomerulus ultrastructure GABA-ergic mossy terminals 


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  1. Alvarado-Mallart RM, Sotelo C (1982) Differentiation of cerebellar anlage heterotropically transplanted to adult rat brain: a light and electron microscopic study. J Comp Neurol 212:247–267Google Scholar
  2. Björklund H, Bickford P, Dahl D, Hoffer B, Olson L (1984) Intracranial cerebellar grafts: intermediate filament immunohistochemistry and electrophysiology. Exp Brain Res 55:372–385Google Scholar
  3. Chan-Palay V (1977) Cerebellar dentate nucleus. Organization, cytology and transmitters. Springer, Berlin Heidelberg New YorkGoogle Scholar
  4. Dietrichs E, Walberg F (1979) The cerebellar corticonuclear and nucleocortical projections in the cat as studied with anterograde and retrograde transport of horseradish peroxidase. I. The paramedian lobule. Anat Embryol 158:13–39Google Scholar
  5. Eccles J, Ito M, Szentágothai J (1967) The cerebellum as a neuronal machine. Springer, Berlin Heidelberg New YorkGoogle Scholar
  6. Gabbott PLA, Somogyi J, Stewart MG, Hámori J (1986) GABA-immunoreactive neurons in the rat cerebellum: a light and electron microscope study. J Comp Neurol 251:474–490Google Scholar
  7. Gray EG (1961) The granule cells, mossy synapses and Purkinje spine synapes of the cerebellum: Light and electron microscope observations. J Anat 95:345–356Google Scholar
  8. Hallas BH, Oblinger M, Das GD (1980) Heterotropic neural transplants in the cerebellum of the rat: their afferents. Brain Res 196:242–246Google Scholar
  9. Hámori J, Lakos I (1979) Synaptic neoformations by Golgi and granule cells following deafferentation of developing cerebellar cortex. Neurosci Lett 3:363Google Scholar
  10. Hámori J, Mezey É, Szentágothai J (1981) Electron microscopic identification of cerebellar nucleocortical mossy terminals in the rat. Exp Brain Res 44:97–100Google Scholar
  11. Hámori J, Somogyi J (1982) Presynaptic dendrites-and perikarya in deafferented cerebellar cortex. Proc Natl Acad Sci USA 79:5093–5096Google Scholar
  12. Hámori J, Takács J (1988) Morphological study of transplanted cerebellar cortex cocultivated with cerebral cortex in anterior eye chamber. II. Purkinje cells and molecular layer. Anat Embryol (in press)Google Scholar
  13. Hodgson AJ, Penke B, Erdei A, Chubb IW, Somogyi P (1985) Antisera to γ-aminobutyric acid. I. Production and characterization using a new model system. J. Histochem Cytochem 33:229–239Google Scholar
  14. Hoffer B, Seigert A, Ljunberg T, Olson L (1974) Electrophysiologycal and cytological studies of brain homografts in the anterior chamber of the eye: Maturation of cerebellar cortex in oculo. Brain Res 79:165–184Google Scholar
  15. Jaeger CB, Lund LD (1979) Efferent fibers from transplanted cerebral cortex of rats. Brain Res 165:338–342Google Scholar
  16. Kromer LF, Björklund A, Stenevi U (1979) Intracephalic implants: a technique for studying neural interactions. Science 204:1117–1119Google Scholar
  17. Kromer LF, Björklund A, Stenevi U (1983) Intracephalic embryonic neural implants in the adult rat brain. I. Growth and mature organization of brainstem, cerebellar and hippocampal implants. J Comp Neurol 218:433–459Google Scholar
  18. Llinas R, Hillman DE, Precht W (1973) Neuronal circuit reorganization in mammalian agranular cerebellar cortex. J Neurobiol 4:69–94Google Scholar
  19. May R, LaGreffe M (1954) Brephoblastique intraoculaire du cervelet chez la souris. Arch Anat Microsc Morphol Exp 43:42–57Google Scholar
  20. Oblinger MM, Hallas BH, Das GD (1980) Neocortical transplants in the cerebellum of the rat: their afferents and efferrents. Brain Res 189:228–232Google Scholar
  21. Olson L, Seiger A (1972) Brain tissue transplanted to the anterior chamber of the eye. I. Fluorescence histochemistry of immature catecholamine and 5-hydroxytryptamine neurons reinnervating the rat iris. Z Zellforsch 135:175–194Google Scholar
  22. Olson L, Seiger A, Freedman R, Hoffer B (1980) Chromaffine cells can innervate brain tissue: evidence from intraocular double grafts. Exp Neurol 70:414–426Google Scholar
  23. Olson L, Björklund H, Hoffer BJ (1984) Camera bulbi anterior. New visitas on a classical locus for neural tissue transplantation. In: Sladek JR, Gash DM (eds) Neural transplants, Plenum Press, New YorkGoogle Scholar
  24. Olson L, Vanderhaeghen JJ, Freedman R, Henschen A, Hoffer B, Seiger A (1985) Combined grafts of the ventral tagmental area and nucleus accumbens in oculo. Histochemical and electrophysiological characterization. Exp Brain Res 59:325–337Google Scholar
  25. Palay SL, Chan-Palay V (1974) Cerebellar cortex, cytology and organization. Springer, Berlin Heidelberg New YorkGoogle Scholar
  26. Palmer MR, Björklund H, Freedman R, Taylor DA, Marwaha J, Olson L, Seiger A, Hoffer BJ (1981) Permanent impairment of spontaneous Purkinje cell discharge in cerebellar grafts caused by chronic lead exposure. Toxicol Appl Pharmacol 60:431–448Google Scholar
  27. Seiger A, Olson L (1975) Brain tissue transplanted to the anterior chamber of the eye. 3. Substitution of lacking central noradrenaline input by host iris sympathetic fibers in the isolated cerebral cortex developed in oculo. Cell Tissue Res 159:325–338Google Scholar
  28. Somogyi P, Hodgson AJ (1985) Antisera to γ-aminobutyric acid. III. Demonstration of GABA in Golgi-impregnated neurons and in conventional electron microscopic sections of cat striate cortex. J Histochem Cytochem 33:249–257Google Scholar
  29. Sotelo C (1975) Anatomical, physiological and biochemical studies of the cerebellum from mutant mice. II. Morphological study of cerebellar cortical neurons and circuits in the weaver mouse. Brain Res 94:19–44Google Scholar
  30. Takács J, Tran Minh Nhon, Hámori J (1987) Electronmicroscopical study of synaptic glomeruli in cerebellum transplanted to the anterior eye chamber. Acta Biol Hung (in press)Google Scholar
  31. Wilson L, Sotelo C, Caviness VS Jr (1981) Heterologous synapses upon Purkinje cells in the cerebellum of the reeler mutant mouse: An experimental light and electron microscopic study. Brain Res 213:63–82Google Scholar
  32. Woodward DJ, Seiger A, Olson L, Hoffer BJ (1977) Intrinsic and extrinsic determinants of dendritic development as revealed by Golgi studies of cerebellar and hippocampal transplants in oculo. Exp Neurol 57:984–998Google Scholar
  33. Zhuravleva ZN, Bragin AG, Vinogradova OS (1984) Organization of the nervous tissue (hippocampus and septum) developing in the anterior eye chamber. I. General characteristics and nonneural elements. J Hirnforsch 25:313–330Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • J. Takács
    • 1
    • 2
  • J. Hámori
    • 1
    • 2
  1. 1.Neurobiology Research Laboratory of the Hungarian Academy of SciencesBudapestHungary
  2. 2.Semmelweis UniversityBudapestHungary

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