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The feline oculomotor nucleus: morphological subdivisions and projection to the cerebellar cortex and nuclei

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Summary

The cytoarchitecture of the feline oculomotor nucleus was examined in sections stained with thionin and neutral red. Five different subdivisions (caudal central, paramedian, ventral, dorsomedial and dorsolateral divisions) can be identified on each side of the midline. This observation is discussed, and our findings are compared to previous studies of the cytoarchitecture or central muscular representation of the oculomotor nucleus in which different subgroups have been distinguished. Implants or injections of the wheat germ agglutinin-horseradish peroxidase complex have revealed that all five subdivisions project to different parts of the cerebellar cortex and nuclei. Retrogradely labelled cells were found in the oculomotor nucleus in 18 cases following deposition of tracer in the fastigial and interposed nuclei and certain regions of the anterior, posterior and flocculonodular lobes. The projection is bilateral and appears to have its main termination in flocculus. It originates from small neurons, especially from those located along the dorsal border of the oculomotor nucleus.

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References

  • Akagi Y (1978) The localization of the motor neurons innervating the extraocular muscles in the oculomotor nuclei of the cat and rabbit, using horseradish peroxidase. J Comp Neurol 181:745–762

    Google Scholar 

  • Appenteng K, Girdlestone D (1987) Transneuronal transport of wheat germ agglutinin-conjugated horseradish peroxidase into trigeminal interneurones of the rat. J Comp Neurol 258:387–396

    Google Scholar 

  • Broadwell RD, Balin BJ (1985) Endocytic and exocytic pathways of the neuronal secretory process and transsynaptic transfer of wheat germ agglutinin-horseradish peroxidase in vivo. J Comp Neurol 242:632–650

    Google Scholar 

  • Brodal A (1981) Neurological anatomy in relation to clinical medicine. Oxford University Press, New York London

    Google Scholar 

  • Brodal A, Lacerda AM, Destombes J, Angaut P (1972) The pattern in the projection of the intracerebellar nuclei onto the nucleus reticularis tegmenti pontis in the cat. An experimental anatomical study. Exp Brain Res 16:140–160

    Google Scholar 

  • Brodal P, Dietrichs E, Bjaalie JG, Nordby T, Walberg F (1983) Is lectin-coupled horseradish peroxidase taken up and transported by undamaged as well as by damaged fibers in the central nervous system? Brain Res 278:1–9

    Google Scholar 

  • Brown JO (1943) The nuclear pattern of the non-tectal portions of the midbrain and isthmus in the dog and cat. J Comp Neurol 78:365–405

    Google Scholar 

  • Büttner U, Boyle R, Markert G (1986) Cerebellar control of eye movements. Prog Brain Res 64:225–234

    Google Scholar 

  • Carpenter MB, Strominger NL (1964) Cerebello-oculomotor fibers in the rhesus monkey. J Comp Neurol 123:211–230

    Google Scholar 

  • Corvaja N, Grofová I, Pompeiano O, Walberg F (1977) The lateral reticular nucleus in the cat — I. An experimental anatomical study of its spinal and supraspinal afferent connections. Neuroscience 2:537–553

    Google Scholar 

  • Dietrichs E, Walberg F (1979) The cerebellar projection from the lateral reticular nucleus as studied with retrograde transport of horseradish peroxidase. Anat Embryol 155:273–290

    Google Scholar 

  • Dietrichs E, Walberg F (1983) Cerebellar cortical afferents from the red nucleus in the cat. Exp Brain Res 50:353–358

    Google Scholar 

  • Dietrichs E, Walberg F (1987) Cerebellar nuclear afferents — where do they originate? A re-evaluation of the projections from some lower brain stem nuclei. Anat Embryol 177:165–172

    Google Scholar 

  • Dietrichs E, Walberg F, Nordby T (1985) The cerebellar nucleo-olivary and olivo-cerebellar nuclear projections in the cat as studied with anterograde and retrograde transport in the same animal after implantation of crystalline WGA-HRP. I. The dentate nucleus. Neurosci Res 3:52–70

    Google Scholar 

  • Evinger C, Graf WM, Baker R (1987) Extra-and intracellular HRP analysis of the organization of extraocular motoneurons and internuclear neurons in the guinea pig and rabbit. J Comp Neurol 262:429–445

    Google Scholar 

  • Gacek RR (1974) Localization of neurons supplying the extraocular muscles in the kitten using horseradish peroxidase. Exp Neurol 44:381–403

    Google Scholar 

  • Gibson AR, Hansma DI, Houk JC, Robinson FR (1984) A sensitive low artifact TMB procedure for the demonstration of WGA-HRP in the CNS. Brain Res 298:235–241

    Google Scholar 

  • Gonatas NK, Harper C, Mizutani T, Gonatas JO (1979) Superior sensitivity of conjugates of horseradish peroxidase with wheat germ agglutinin for studies of retrograde axonal transport. J Histochem Cytochem 27:728–734

    Google Scholar 

  • Gould BB (1980) Organization of afferents from the brain stem nuclei to the cerebellar cortex in the cat. Adv Anat Embryol Cell Biol 62:1–90

    Google Scholar 

  • Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubule of mouse kidney: Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302

    Google Scholar 

  • Graybiel AM (1977) Organization of oculomotor pathways in the cat and rhesus monkey. Dev Neurosci 1:79–88

    Google Scholar 

  • Harrison PJ, Hultborn H, Jankowska E, Katz R, Storai B, Zytnicki D (1984) Labelling of interneurons by retrograde transsynaptic transport of horseradish peroxidase from motoneurons in rats and cats. Neurosci Lett 45:15–19

    Google Scholar 

  • Hoddevik GH (1978) The projection from nucleus reticularis tegmenti pontis onto the cerebellum in the cat. A study using the methods of anterograde degeneration and retrograde axonal transport of horseradish peroxidase. Anat Embryol 153:227–242

    Google Scholar 

  • Ito M (1984) The cerebellum and neural control. Raven Press, New York

    Google Scholar 

  • Kotchabhakdi N, Walberg F (1977) Cerebellar afferents from neurons in motor nuclei of cranial nerves demonstrated by retrograde axonal transport of horseradish peroxidase. Brain Res 137:158–163

    Google Scholar 

  • Kotchabhakdi N, Hoddevik GH, Walberg F (1978) Cerebellar afferent projections from the perihypoglossal nuclei: An experimental study with the method of retrograde axonal transport of horseradish peroxidase. Exp Brain Res 31:13–29

    Google Scholar 

  • Kristensson K, Olsson Y (1971) Retrograde axonal transport of protein. Brain Res 29:363–365

    Google Scholar 

  • La Vail JH, La Vail MM (1972) Retrograde axonal transport in the central nervous system. Science 176:1416–1417

    Google Scholar 

  • Larsell O (1970) The comparative anatomy and histology of the cerebellum from monotremes through apes. Jansen J (ed) University of Minnesota Press, Minneapolis

    Google Scholar 

  • Le Gros Clark WE (1926) The mammalian oculomotor nucleus. J Anat 60:426–448

    Google Scholar 

  • Maciewicz R, Phipps BS (1983) The oculomotor internuclear pathway: A double retrograde labeling study. Brain Res 262:1–8

    Google Scholar 

  • Maciewicz RJ, Kaneko CRS, Highstein SM, Baker R (1975) Morphophysiological identification of interneurons in the oculomotor nucleus that project to the abducens nucleus in the cat. Brain Res 96:60–65

    Google Scholar 

  • Maciewicz RJ, Romagnano MA, Baker R, Highstein SM (1977) Two projections of the oculomotor internuclear neurons. Anat Rec 187:642–643

    Google Scholar 

  • Maciewicz R, Phipps BS, Foote WE, Aronin N, DiFiglia M (1983) The distribution of substance P-containing neurons in the cat Edinger-Westphal nucleus: Relationship to efferent projection systems. Brain Res 270:217–230

    Google Scholar 

  • McCrea RA, Baker R (1985) Anatomical connections of the nucleus prepositus of the cat. J Comp Neurol 237:377–407

    Google Scholar 

  • Mesulam M-M (1978) Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: A non-carcinogenic blue reaction-product with superior sensitivity for visualizing neuronal afferents and efferents. J Histochem Cytochem 26:106–117

    Google Scholar 

  • Mesulam M-M (1982) Principles of horseradish peroxidase neurohistochemistry and their applications for tracing neural pathways-axonal transport, enzyme histochemistry and light microscopic analysis. In: Mesulam M-M (ed) Tracing neural connections with horseradish peroxidase, pp 1–151, John Wiley, Chichester

    Google Scholar 

  • Miyazaki S (1985) Location of motoneurons in the oculomotor nucleus and the course of their axons in the oculomotor nerve. Brain Res 348:57–63

    Google Scholar 

  • Mori J, Hori N, Katsuda N (1981) A new method for application of horseradish peroxidase into a restricted area of the brain. Brain Res Bull 6:19–22

    Google Scholar 

  • Niimi H, Fujiwara N, Takimoto T, Matsugi S (1962) The course and termination of the ascending fibers of the brachium conjunctivum in the cat as studied by the Nauta method. Tokushima J Exp Med 8:269–284

    Google Scholar 

  • Perlia H (1889) Die Anatomie des Oculomotorius beim Menschen. Graefes Arch Clin Exp Ophthalmol 35:287–308

    Google Scholar 

  • Porter JD, Guthrie BL, Sparks DL (1985) Selective retrograde transneuronal transport of wheat germ agglutinin-conjugated horseradish peroxidase in the oculomotor system. Exp Brain Res 57:411–416

    Google Scholar 

  • Pugh WW, Kalia M (1982) Differential uptake of peroxidase (HRP) and peroxidase-lectine (HRP-WGA) conjugate injected in the nodose ganglion of the cat. J Histochem Cytochem 30:887–894

    Google Scholar 

  • Qvist H (1987) Afferents to the lateral reticular nucleus from the oculomotor region. II. The oculomotor nucleus, the interstitial nucleus of Cajal and the nucleus of the posterior commissure. Anat Embryol 177:277–283

    Google Scholar 

  • Saint-Cyr JA (1983) The projection from the motor cortex to the inferior olive in the cat. An experimental study using axonal transport techniques. Neuroscience 10:667–684

    Google Scholar 

  • Steiger H-J, Büttner-Ennever JA (1979) Oculomotor nucleus afferents in the monkey demonstrated with horseradish peroxidase. Brain Res 160:1–15

    Google Scholar 

  • Taber E (1961) The cytoarchitecture of the brain stem of the cat. J Comp Neurol 116:27–70

    Google Scholar 

  • Takada M, Hattori T (1986) Transneuronal transport of WGA-HRP from the ipsi-to the contralateral medial habenular nucleus through the interpeduncular nucleus in the rat. Brain Res 384:77–83

    Google Scholar 

  • Tarlov E, Tarlov SR (1971) The representation of extraocular muscles in the oculomotor nuclei: Experimental studies in the cat. Brain Res 34:37–52

    Google Scholar 

  • Tsuchida U (1906) Über die Ursprungskerne der Augenbewegungs-nerven und über die mit diesen in Beziehung stehenden Bahnen im Mittel-und Zwischenhirn. Normal-anatomische, embryologische, pathologisch-anatomische und vergleichend-anatomische Untersuchungen. Hirnanat Inst Zürich 2:1–205

    Google Scholar 

  • Walberg F, Nordby T, Dietrichs E (1987) The olivonodular projection: A re-examination based on folial cerebellar implants. Neurosci Lett 81:82–88

    Google Scholar 

  • Wan X-C, Trojanowski JQ, Gonatas JO (1982) Cholera toxin and wheat germ agglutinin conjugates as neuroanatomical probes: Their uptake and clearance, transganglionic and retrograde transport and sensitivity. Brain Res 243:215–224

    Google Scholar 

  • Warwick R (1953) Representation of the extra-ocular muscles in the oculomotor nuclei of the monkey. J Comp Neurol 98:449–504

    Google Scholar 

  • Yingcharoen K, Rinvik E (1982) Branched projections from the nucleus prepositus hypoglossi to the oculomotor nucleus and the cerebellum. A retrograde fluorescent double-labeling study in the cat. Brain Res 246:133–136

    Google Scholar 

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  1. Anatomical Institute, University of Oslo, Karl Johansgt. 47, N-0162, Oslo, Norway

    Geir Ketil Røste & Espen Dietrichs

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  1. Geir Ketil Røste
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  2. Espen Dietrichs
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Røste, G.K., Dietrichs, E. The feline oculomotor nucleus: morphological subdivisions and projection to the cerebellar cortex and nuclei. Anat Embryol 178, 67–75 (1988). https://doi.org/10.1007/BF00305016

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