Summary
The distribution of binding sites for Dolichos biflorus agglutinin (DBA) was studied by histochemical staining in the embryonic mouse liver. The liver primordium consists of cranial and caudal diverticula. DBA bound to the pseudostratified endodermal cells of the caudal hepatic diverticulum, and also to some endodermal cells of the cranial one. Most extrahepatic bile duct cells and all epithelial cells of the gall bladder were consistently DBA-positive. In intrahepatic bile ducts and their precursors, the DBA binding sites showed a patchy distributon. Most hepatocytes were DBA-negative, though some young hepatocytes were DBA-positive. The results suggest that DBA binding sites are useful markers of epithelial cells of the gall bladder and the bile ducts, especially the extrahepatic bile duct. Differentiation of bile duct cells was also discussed with special reference to the developmental change of the distribution of the DBA binding sites.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Du Bois AM (1963) The embryonic liver. In: Rouiller C (ed) The liver, morphology, biochemistry, physiology, vol 1. Academic Press, New York London, pp 1–39
Etzler ME, Kabat EA (1970) Purification and characterization of a lectin (plant hemagglutinin) with blood group A specificity from Dolichos biflorus. Biochemistry 9:869–877
Fukuda S, Mizuno T (1978) Hepatic parenchyma, biliary ducts and gall bladder forming potency in the hepatic primordium in the quail embryo. Anat Embryol 155:15–21
Hammar JA (1926) Über die erste Entstehung der nicht kapillaren intrahepatischen Gallengänge beim Menschen. Z Mikrosk Anat Forsch 5:59–89
Horstmann E (1939) Entwicklung und Entwicklungsbedingungen des intrahepatischen Gallengangsystems. Wilhelm Roux' Arch EntwMech Org 139:363–392
Koga A (1971) Morphogenesis of intrahepatic bile ducts of the human fetus. Light and electron microscopical study. Z Anat EntwickGesch 135:156–184
Nakane PK (1968) Simultaneous localization of multiple tissue antigens using the peroxidase-labelled antibody method: A study on pituitary glands of the rat. J Histochem Cytochem 16:557–560
Ponder BAJ, Festing MFW, Wilkinson MM (1985) An allelic difference determines reciprocal patterns of expression of binding sites for Dolichos biflorus lectin in inbred strains of mice. J Embryol Exp Morphol 87:229–239
Shiojiri N (1979) The differentiation of the hepatocytes and the intra- and extrahepatic bile duct cells in mouse embryos. J Fac Sci Univ Tokyo IV 14:241–250
Shiojiri N (1981) Enzymo- and immunocytochemical analyses of the differentiation of liver cells in the prenatal mouse. J Embryol Exp Morphol 62:139–152
Shiojiri N (1984) The origin of intrahepatic bile duct cells in the mouse. J Embryol Exp Morphol 79:25–39
Shiojiri N, Katayama H (1987) Secondary joining of the bile ducts during the hepatogenesis of the mouse embryo. Anat Embryol (in press)
Watanabe M, Muramatsu T, Shirane H, Ugai K (1981) Discrete distribution of binding sites for Dolichos biflorus agglutinin (DBA) and for peanut agglutinin (PNA) in mouse organ tissues. J Histochem Cytochem 29:779–790
Wilson JW, Groat CS, Leduc EH (1963) Histogenesis of the liver. Ann NY Acad Sci 111:8–24
Wood RL (1965) An electron microscope study of developing bile canaliculi in the rat. Anat Rec 151:507–530
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shiojiri, N., Katayama, H. Development of Dolichos biflorus agglutinin (DBA) binding sites in the bile duct of the embryonic mouse liver. Anat Embryol 178, 15–20 (1988). https://doi.org/10.1007/BF00305009
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00305009