Summary
Cells from thymus and/or bone marrow tissues of either normal or Gross virus-infected W/Fu rats were cultivated individually or mixed. Four continuous cell lines were obtained: 1) individual cultivation of the cells from thymic lymphoma, 2) combined cultivation of cells from thymic lymphoma and normal bone marrow, 3) bone marrow of a leukemic rat and normal thymus, and 4) bone marrow of a leukemic rat supplemented with cell-free extracts of normal thymus homogenates.
The morphological features were similar in these 4 cell lines. The cells correspond morphologically to lymphoblasts, grow rapidly in suspension, and are transplantable in newborn rats. The cells release abundant Type-C virus particles. The inoculation of cell-free culture fluid into newborn rats resulted, after 7–9 weeks, in the development of thymic lymphoma in high incidence.
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This work was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture, Japan.
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Nishiyama, T., Okano, H. Establishment and characterization of cell lines from gross virus-induced rat leukemia. Z. Krebsforsch. 86, 13–22 (1976). https://doi.org/10.1007/BF00304930
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DOI: https://doi.org/10.1007/BF00304930