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Artefakte im Amylase-Substratfilmtest

Artifacts in the substrate film test for amylase

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Summary

In substrate film tests for amylase, periodate-oxidized starch films (Shear and Pearse, 1963) may produce artifacts, for they undergo spontaneous hydrolysis at slightly alkaline pH levels. This is concluded from observations made on rabbit blastocysts.

Zusammenfassung

Bei der Verwendung von perjodsäureoxydierten Stärkefilmen (Shear und Pearse, 1963) zum Amylasenachweis können Artefakte entstehen durch alkalische, nicht-enzymatische Hydrolyse in Geweben mit hohem pH. Dies wird anhand von Beobachtungen an Kaninchen-Blastozysten erläutert.

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Literatur

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Authors and Affiliations

  1. Anatomisches Institut der Universität Marburg a. d. Lahn und Arbeitsgruppe Prof. Dr. G. H. M. Gottschewski am Max-Planck-Institut für Immunbiologie Freiburg i. Br., Germany

    H. W. Denker

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  1. H. W. Denker
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Denker, H.W. Artefakte im Amylase-Substratfilmtest. Histochemie 21, 17–20 (1970). https://doi.org/10.1007/BF00304801

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  • DOI: https://doi.org/10.1007/BF00304801

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