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Specfic staining of 9h in human somatic interphase cells by D 287/170

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Summary

Somatic interphase cells from males and females with normal karyotypes and with variants of the heterochromatic regions on chromosomes 9 and Y were stained with the fluorochrome D287/170. The results showed that only 9h retained the ability to stain with D 287/170 in the interphase state, whereas 15ph and Yqh lost the specific staining pattern seen in metaphase. The number and size of the specific stained interphase bodies correlated with the ploidy of the cell population and the size of the 9h as seen in metaphase.

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References

  • Beauchamp RS, Mitchell AR, Buckland RA, Bostock CJ (1979) Speicific arrangements of human satellite III DNA sequences in human chromosomes. Chromosoma 71:153–166

    Google Scholar 

  • Bobrow M, Madan K, Pearson PL (1972) Staining of some specific regions of human chromosomes, particularly the secondary constriction of No. 9. Nature New Biol 238:122–124

    Google Scholar 

  • Cooke H (1976) Repeated sequence specific to human males. Nature 262:182–186

    Google Scholar 

  • Gagnè H, Luciani JM, Devictor-Viullet M, Stahl A (1974) C9 heterochromatin during the first meiotic prophase of human foetal oocyte. Exp Cell Res 85:111–116

    Google Scholar 

  • Gosden JR, Mitchell AR, Buckland RA, Clayton RP, Evans HJ (1975) The location of four human satellite DNAs on human chromosomes. Exp Cell Res 92:148–158

    Google Scholar 

  • Gosden JR, Spowart G, Lawrie SS (1981a) Satellite DNA and cytological staining patterns in heterochromatic inversions of human chromosome 9. Hum Genet 58:276–278

    Google Scholar 

  • Gosden JR, Lawrie SS, Cooke HJ (1981b) A cloned repeated DNA sequence in human chromosome heteromorphisms. Cytogenet Cell Genet 29:32–39

    Google Scholar 

  • Hungerford DA, Ashton FT, Balaban GB, LaBadie GU, Messatzzia LR, Haller G, Miller AE (1972) The C-group pachytene bivalent with a locus characteristic for parachromosomally situated particulate bodies (parameres): A provisional map in human males. Proc Natl Acad Sci USA 69:2165–2168

    Google Scholar 

  • Lubit BW, Schreck RR, Miller OJ, Erlanger BF (1974) Human chromosome structure as revealed by an immunoperoxidase staining procedure. Exp Cell Res 89:426–429

    Google Scholar 

  • Macaya G, Thiery J-P, Bernadi G (1977) DNA sequences in man. In: Yunis JJ (ed) Molecular structure of human chromosomes. Academic Press, New York, pp 35–58

    Google Scholar 

  • Miller OJ, Schnedl W, Allen J, Erlanger BF (1974) 5-Methyl-cytosine localised in mammalian constitutive heterochromatin. Nature 251: 636–637

    Google Scholar 

  • Miller OJ, Miller DA, Erlanger BF (1982) Demethylation of mouse satellite DNA in spermiogenesis. Abstract, 1982, Meeting of the Genetics Society of America, New Haven. Genetics 100 [Suppl] 1/2:48

    Google Scholar 

  • Schnedl W, Mikelsaar A-V, Breitenbach M, Dann O (1977) DIPI and DAPI: Fluorescence banding with only negligible fading. Hum Genet 36:167–172

    Google Scholar 

  • Schnedl W, Abraham R, Dann O, Geber G, Schweitzer D (1981) Preferential fluorescent staining of heterochromatic regions in human chromosomes 9, 15 and the Y by D287/170. Hum Genet 59:10–13

    Google Scholar 

  • Schweizer D (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58:307–324

    Google Scholar 

  • Schweizer D, Ambros P, Andrle M (1978) Modification of DAPl banding on human chromosomes by prestaining with a DNA-binding oligopeptide antibiotic, distamycin A. Exp Cell Res 111:327–332

    Google Scholar 

  • Sturm KS, Taylor JH (1981) Distribution of 5-methylcytosine in the DNA of somatic and germline cells from bovine tissues. Nucl Acids Res 9:4537–4546

    Google Scholar 

  • Vanyushin BF, Tkacheva SG, Belozersky AN (1970) Rare bases in animal DNA. Nature 225:948–949

    Google Scholar 

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Tommerup, N. Specfic staining of 9h in human somatic interphase cells by D 287/170. Hum Genet 62, 301–304 (1982). https://doi.org/10.1007/BF00304543

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  • DOI: https://doi.org/10.1007/BF00304543

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