Summary
Somatic interphase cells from males and females with normal karyotypes and with variants of the heterochromatic regions on chromosomes 9 and Y were stained with the fluorochrome D287/170. The results showed that only 9h retained the ability to stain with D 287/170 in the interphase state, whereas 15ph and Yqh lost the specific staining pattern seen in metaphase. The number and size of the specific stained interphase bodies correlated with the ploidy of the cell population and the size of the 9h as seen in metaphase.
Similar content being viewed by others
References
Beauchamp RS, Mitchell AR, Buckland RA, Bostock CJ (1979) Speicific arrangements of human satellite III DNA sequences in human chromosomes. Chromosoma 71:153–166
Bobrow M, Madan K, Pearson PL (1972) Staining of some specific regions of human chromosomes, particularly the secondary constriction of No. 9. Nature New Biol 238:122–124
Cooke H (1976) Repeated sequence specific to human males. Nature 262:182–186
Gagnè H, Luciani JM, Devictor-Viullet M, Stahl A (1974) C9 heterochromatin during the first meiotic prophase of human foetal oocyte. Exp Cell Res 85:111–116
Gosden JR, Mitchell AR, Buckland RA, Clayton RP, Evans HJ (1975) The location of four human satellite DNAs on human chromosomes. Exp Cell Res 92:148–158
Gosden JR, Spowart G, Lawrie SS (1981a) Satellite DNA and cytological staining patterns in heterochromatic inversions of human chromosome 9. Hum Genet 58:276–278
Gosden JR, Lawrie SS, Cooke HJ (1981b) A cloned repeated DNA sequence in human chromosome heteromorphisms. Cytogenet Cell Genet 29:32–39
Hungerford DA, Ashton FT, Balaban GB, LaBadie GU, Messatzzia LR, Haller G, Miller AE (1972) The C-group pachytene bivalent with a locus characteristic for parachromosomally situated particulate bodies (parameres): A provisional map in human males. Proc Natl Acad Sci USA 69:2165–2168
Lubit BW, Schreck RR, Miller OJ, Erlanger BF (1974) Human chromosome structure as revealed by an immunoperoxidase staining procedure. Exp Cell Res 89:426–429
Macaya G, Thiery J-P, Bernadi G (1977) DNA sequences in man. In: Yunis JJ (ed) Molecular structure of human chromosomes. Academic Press, New York, pp 35–58
Miller OJ, Schnedl W, Allen J, Erlanger BF (1974) 5-Methyl-cytosine localised in mammalian constitutive heterochromatin. Nature 251: 636–637
Miller OJ, Miller DA, Erlanger BF (1982) Demethylation of mouse satellite DNA in spermiogenesis. Abstract, 1982, Meeting of the Genetics Society of America, New Haven. Genetics 100 [Suppl] 1/2:48
Schnedl W, Mikelsaar A-V, Breitenbach M, Dann O (1977) DIPI and DAPI: Fluorescence banding with only negligible fading. Hum Genet 36:167–172
Schnedl W, Abraham R, Dann O, Geber G, Schweitzer D (1981) Preferential fluorescent staining of heterochromatic regions in human chromosomes 9, 15 and the Y by D287/170. Hum Genet 59:10–13
Schweizer D (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58:307–324
Schweizer D, Ambros P, Andrle M (1978) Modification of DAPl banding on human chromosomes by prestaining with a DNA-binding oligopeptide antibiotic, distamycin A. Exp Cell Res 111:327–332
Sturm KS, Taylor JH (1981) Distribution of 5-methylcytosine in the DNA of somatic and germline cells from bovine tissues. Nucl Acids Res 9:4537–4546
Vanyushin BF, Tkacheva SG, Belozersky AN (1970) Rare bases in animal DNA. Nature 225:948–949
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tommerup, N. Specfic staining of 9h in human somatic interphase cells by D 287/170. Hum Genet 62, 301–304 (1982). https://doi.org/10.1007/BF00304543
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00304543