Skip to main content
SpringerLink
Log in

The localization of 125iodide, 3H-inulin and 3H-acetylcholine in autoradiographic model experiments

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

Autoradiographic experiments are described in which low and high molecular radioactive compounds with different types of radiation are used. The effect of the following procedures on the spread of grains were studied: 1) cutting-direction, 2) diffusion of molecules at cryostate temperatures, 3) the type of radiation, 4) time lapse between dissection and freezing of the tissue.

It is suggested that the resolution with 125Iodine-labeled compounds is worse than with tritium-labeled compounds, owing to different radiation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Appleton, T. C.: Stripping film autoradiography. In: Autoradiography for biologists, edited by P. B. Gahan, p. 48. London-New York: Academic Press 1972.

    Google Scholar 

  • Brown, D. A., Stumpf, W. E., Diab, I. M., Roth, L. J.: Assessment of autoradiographic resolution using soluble extracellular fluid indicators. In: Autoradiography of diffusible substances, edited by L. J. Roth, W. E. Stumpf, p. 161–182. New York-London: Academic Press 1969.

    Google Scholar 

  • Doniach, I., Pelc, S. R.: Autoradiographic technic. Brit. J. Radiol. 23, 184–192 (1950).

    Google Scholar 

  • Fischer, H. A., Werner, G.: Eine Methode zur quantitativen Auswertung von Autoradiogrammen auf der Grundlage der Durchlicht-Mikro-Photometrie. Histochemie 15, 84–92 (1968).

    Google Scholar 

  • Medical Internal Radiation Dose Committee: J. Nucl. Med. supplement number 2, pamphlet number 4. March 1969.

  • Pelc, S. R.: Theory of autoradiography. In: Autoradiography for biologists, edited by P. B. Gahan, p. 8–10. London-New York: Academic Press 1972.

    Google Scholar 

  • Polak, R. L.: The influence of drugs on the uptake of acetyl-choline by slices of rat cerebral cortex. Brit. J. Pharmacol. 36, 144–152 (1969).

    Google Scholar 

  • Rogers, W. A.: Techniques of autoradiography, p. 324. Amsterdam-London-New York: Elsevier Publishing Co. 1967.

    Google Scholar 

  • Stumpf, W. E.: Tissue preparation for the autoradiographic localization of hormones. In: Introduction to quantitative cytochemistry, edited by G. L. Wied, vol. 2, p. 507–526. New York: Academic Press Inc. (1970).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Pharmacology and Cell Biology, University of Leiden, The Netherlands

    O. M. J. Driessen, C. W. Thesingh & N. van den Bosch

Authors
  1. O. M. J. Driessen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. W. Thesingh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. van den Bosch
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Driessen, O.M.J., Thesingh, C.W. & van den Bosch, N. The localization of 125iodide, 3H-inulin and 3H-acetylcholine in autoradiographic model experiments. Histochemie 36, 115–121 (1973). https://doi.org/10.1007/BF00304387

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00304387

Keywords

Search

Navigation