Summary
Skin autografting or a single painting with the cocarcinogen TPA was used to induce epidermal hyperplasia in the back skin of C3H mice.
Initiation by intragastric application of the carcinogen DMBA during this state of hyperplasia and subsequent promotion by repeated application of the cocarcinogen TPA led to decreased papilloma-formation, as compared to mice of a control group which had not been pretreated before initiation. Reports by others referring to increased susceptibility of replicating epidermal cells to the effect of initiation thus cannot be confirmed.
The reduction of papilloma-formation can most probably be ascribed to effects of local inflammation, either preferentially but unspecifically damaging initiated cells, or facilitating a specific immune response against tumor-associated transplantation antigens of prospective tumor cells.
Zusammenfassung
Durch eine Hautautotransplantation oder eine einmalige lokale Gabe des Cocarcinogens TPA wurde in der Rückenhaut von C3H Mäusen eine epidermale Hyperplasie induziert.
Nach Initiierung durch intragastrische Applikation des Carcinogens DMBA während der Zeit der Hyperplasie und anschließende Dauerbehandlung mit TPA war die Papillombildung deutlich geringer als in der vor der Initiierung nicht behandelten Kontrollgruppe. Berichte anderer Autoren über eine erhöhte Empfänglichkeit von in der Replikation befindlichen epidermalen Zellen gegenüber der initiierenden Wirkung eines Carcinogens können somit nicht bestätigt werden.
Die Reduktion der Papillomausbeute dürfte am ehesten den Effekten der gleichzeitig bestehenden lokalen Entzündung zuzuschreiben sein. Diese könnten entweder bevorzugt initiierte Zellen auf unspezifische Weise schädigen oder aber eine spezifische Abwehrreaktion des Immunsystems gegen tumorassoziierte Transplantationsantigene auf der Oberfläche der zukünftigen Tumorzellen begünstigen.
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Supported by a grant of the Deutsche Forschungsgemeinschaft.
The excellent technical assistance of Miss B. Kissel, Miss G. Schilling, and Mrs. P. Boukamp is gratefully acknowledged.
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Worst, P.K.M., Boukamp, H.K. Skin transplantation in the study of chemical carcinogenesis. Z. Krebsforsch. 84, 97–103 (1975). https://doi.org/10.1007/BF00304036
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DOI: https://doi.org/10.1007/BF00304036