Summary
Wall-bound proteins of Lilium longiflorum pollen tubes grown in vivo constitute 20–27% of the dry matter. Twenty-two-twenty-six percent of these proteins are NaCl soluble. Wall-bound proteins of in vivo pollen tubes are present in amounts 5–7 times that found in tubes grown in vitro. The protein pattern of wall-bound proteins is different between in vitro and in vivo grown pollen tubes. There are two kinds of pollen tube wall proteins: loosely bound and tightly bound. The latter are NaCl insoluble, contain hydroxyproline and are assumed to be covalently bound. No significant differences have been found in the amount of wall-bound proteins present between pollen tubes resulting after self-pollination and those resulting from cross-pollination. However, some band differences between self- and cross pollen tubes have been observed after gel electrophoresis. It can be supposed that some wall-bound proteins of pollen tubes are associated with the incompatibility reaction.
Similar content being viewed by others
References
Campbell RJ, Ascher PD (1975) Incorporation of radioactive label into nucleic acids of compatible and incompatible pollen tubes of Lilium longiflorum Thunb. Theor Appl Genet 46:143–148
Dashek WV, Harwood HI, Rosen WG (1971) The significance of a wall-bound, hydroxyproline-containing glycopeptide in lily pollen tube elongation. In: Heslop-Harrison J (ed) Pollen: development and physiology. Butterworth, London, pp 194–200
Dickinson DB (1968) Rapid starch synthesis associated with increased respiration in germinating lily pollen. Plant Physiol 43:1–8
Dubray G, Bezard G (1982) A highly sensitive periodic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gels. Anal Biochem 119:325–329
Engels FM (1974) Function of Golgi vesicles in relation to cell wall synthesis in germinating Petunia pollen. 4. Identification of cellulose in pollen tube walls and Golgi vesicles by X-ray diffraction. Acta Bot Neerl 23:81–89
Eschenbruch M, Bürk RR (1982) Experimentally improved reliability of ultrasensitive silver staining of protein in polyacrylamide gels. Anal Biochem 125:96–99
Harris JL, Faber WA (1973) Compositional studies on the cell walls of the synnema and vegetative hyphae of Ceratocystis ulmi. Can J Bot 51:1147–1153
Knox RB, Heslop-Harrison J (1970) Pollen-wall proteins: localization and enzymic activity. J Cell Sci 6:1–27
Knox RB, Willing RR, Ashford AA (1972) Role of pollen — wall proteins as recognition substances in interspecific incompatibility in poplars. Nature 237:381–383
Kroh M (1967) Fine structure of Petunia pollen germinated in vivo. Rev Palaeobotan Palynol 3:197–203
Kroh M, Miki-Hirosige H, Rosen W, Loewus F (1970) Incorporation of label into pollen tube walls from myo-inositol-labeled Lilium longiflorum pistils. Plant Physiol 45:92–194
Labarca C, Loewus F (1972) The nutritional role of pistil exudate in pollen tube wall formation in Lilium longiflorum. 1. Utilization of injected stigmatic exudate. Plant Physiol 50:7–14
Labarca C, Loewus F (1973) The nutritional role of pistil exudate in pollen tube wall formation in Lilium longiflorum. 2. Production and utilization of exudate from stigma and stylar canal. Plant Physiol 52:87–92
Linskens HF (1975a) The physiological basis of incompatibility in angiosperms. Biol J Linnean Soc 7 (Suppl) 1:143–152
Linskens HF (1975b) Incompatibility in Petunia. Proc R Soc London, Ser B 188:299–311
Linskens HF (1981) Barreras en la fertilization de las plantas superiores. Kurtziana (Cordoba, Argent) 14:7–20
Linskens HF (1983) Pollination processes: understanding fertilization and limits to hybridization. In: Meudt WJ (ed) Strategies of plant reproduction. Beltville Symposia in Agricultural Research 5. Allanheld-Osmond, Granada, pp 35–50
Linskens HF, Kroh M (1967) Incompatibilität der Phanerogamen. In: Ruhland W (ed) Encyclopedia of plant physiology, vol 18. Springer, Berlin Heidelberg New York, pp 506–530
Li Yi-qin, Croes AF, Linskens HF (1983) Cell wall proteins in pollen and roots of Lilium longiflorum: extraction and partial characterization. Planta 158:422–427
Manocha MS, Ross Colvin J (1967) Structure and composition of the cell wall of Neurospora crassa. J Bacteriol 94:202–212
Mühlethaler K, Linskens HF (1956) Elektronenmikroskopische Aufnahmen von Pollenschläuchen. Experientia 12:253–254
Shirvanna KR, Heslop-Harrison Y, Heslop-Harrison J (1982) The pollen-stigma interaction in the grasses. 3. Features of the self-incompatibility response. Acta Bot Neerl 31:307–319
Talmadge KW, Keegstra K, Bauer WD, Albersheim P (1973) The structure of plant cell walls. 1. The macro-molecular components of the walls of suspension-cultures sycamore cells with a detailed analysis of the pectic polysaccharides. Plant Physiol 51:158–173
Yang ZH, Tsao TH (1981) Physicochemical properties of pollen wall proteins of Cucurbita pepo and Luffa cylindrica and their possible role in ‘recognition’ reaction. Acta Gen Sin 8:75–83
Author information
Authors and Affiliations
Additional information
Communicated by P. L. Pfahler
Rights and permissions
About this article
Cite this article
Li, Y.q., Linskens, H.F. Wall-bound proteins of pollen tubes after self- and cross-pollination in Lilium longiflorum . Theoret. Appl. Genetics 67, 11–16 (1983). https://doi.org/10.1007/BF00303915
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00303915