Skip to main content

Mapping of the Hor-3 locus encoding D hordein in Barley

Summary

The hordein storage proteins of barley (Hordeum vulgare L.) are of intense interest due to their genetic diversity and prominence and impact on the industrial and agricultural uses of the seed. Two major hordein loci have been previously mapped on chromosome 5 (Hor-1 and Hor-2 encoding the C and B hordeins, respectively). A third major locus, Hor-3, which codes for D hordein, has been located in the centromeric region of chromosome 5, probably on the long arm. Two allelic variants with apparent molecular weights of 83,000 and 91,000 and similar isoelectric points of 8.0 comprise the products of this locus in the barley varieties ‘Advance’ and ‘Triple Awned Lemma’. The D hordein examined is similar in molecular weight and isoelectric point to the high molecular weight (HMW) glutenin proteins encoded by the 1B chromosome of wheat (Triticum aestivum L.)

This is a preview of subscription content, access via your institution.

Literature

  1. Allard, R.W. (1956): Formulas and tables to facilitate calculation of recombination values in heredity. Hilgardia 24, 235–278

    Google Scholar 

  2. Burnouf, T.; Bouriquet, R. (1980): Glutenin subunits of genetically related European hexaploid wheat cultivars: their relation to breadmaking quality. Theor. Appl. Genet. 58, 107–111

    Google Scholar 

  3. Cameron-Mills, V. (1980): The structure and composition of protein bodies purified from barley endosperm by silica sol density gradients. Carlsberg Res. Comm. 45, 557–576

    Google Scholar 

  4. Cameron-Mills, V.; von Wettstein, D. (1980): Protein body formation in the developing barley endosperm. Carlsberg Res. Comm. 45, 577–594

    Google Scholar 

  5. Doll, H.; Brown, A.H.D. (1979): Hordein variation in wild (Hordeum spontaneum) and cultivated (H. vulgare) barley. Can. J. Genet. Cytol. 21, 391–404

    Google Scholar 

  6. Doll, H.; Andersen, B. (1981): Preparation of barley storage proteins, hordein, for analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal. Biochem. 115, 61–66

    Google Scholar 

  7. Faulks, A.J.; Shewry, P.R.; Miflin, B.J. (1981): The polymorphism and structural homology of storage polypeptides (hordein) coded by the Hor-2 locus in barley (Hordeum vulgare L.). Biochem. Genet. 19, 841–858

    Google Scholar 

  8. Hash, C.T.; Blake, T.K. (1981): Half-seed determination of hordeins associated with known ML-A alleles conferring race-specific resistance to barley powdery mildew. Barley Genet. Newslett. 11, 74–76

    Google Scholar 

  9. Holder, A.A.; Ingversen, J. (1978): Peptide mapping of the major components of in vitro synthesized barley hordein. Evidence of structural homology. Carlsberg Res. Commun. 43, 177–184

    Google Scholar 

  10. Holt, L.M.; Astin, R.; Payne, P.I. (1981): Structural and genetical studies on the high molecular weight subunits of wheat glutenin. Theor. Appl. Genet. 60, 237–243

    Google Scholar 

  11. Jensen, J. (1981): Coordinator's report, chromosome 5. Barley Genet. Newslett. 11, 87–88

    Google Scholar 

  12. Laemmli, U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685

    Google Scholar 

  13. Lawrence, G.J.; Shepherd, K.W. (1981): Chromosomal location of genes controlling seed proteins in species related to wheat. Theor. Appl. Genet. 59, 25–31

    Google Scholar 

  14. Miflin, B.J.; Burgess, S.R.; Shewry, P.R. (1981): The development of protein bodies in the storage tissues of seeds: subcellular separations of homogenates of barley, maize and wheat endosperms and of maize cotyledons. J. Exp. Bot. 32, 199–219

    Google Scholar 

  15. O'Farrell, P.H. (1975): High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021

    Google Scholar 

  16. Oram, R.N.; Doll, H.; Koie, B. (1975): Genetics of two storage protein variants in barley. Hereditas 80, 53–58

    Google Scholar 

  17. Schmitt, J.M.; Svendsen, I. (1980a): Amino acid sequences of hordein polypeptides. Carlsberg Res. Commun. 45, 143–148

    Google Scholar 

  18. Schmitt, J.M.; Svendsen, I. (1980b): Partial amino acid sequence from hordein polypeptide B1. Carlsberg Res. Commun. 45, 549–555

    Google Scholar 

  19. Shewry, P.R.; Ellis, J.R.S.; Pratt, H.M.; Miflin, B.J. (1978): A comparison of methods for the extraction and separation of hordein fractions from 29 barley varieties. J. Sci. Food Agric. 29, 433–441

    Google Scholar 

  20. Shewry, P.R.; Faulks, A.J.; Pickering, R.A.; Jones, I.T.; Finch, R.A.; Miflin, B.J. (1980a): The genetic control of barley storage proteins. Heredity 44, 383–389

    Google Scholar 

  21. Shewry, P.R.; Field, J.M.; Kirkman, M.A.; Faulks, A.J.; Miflin, B.J. (1980b): The extraction, solubility and characterization of two groups of barley storage polypeptides. J. Exp. Bot. 31, 393–407

    Google Scholar 

  22. Shewry, P.R.; Field, J.M.; Lew, E.J.-L.; Kasarda, D.D. (1982): The purification and characterization of two groups of storage proteins (Secalins) from rye (Secale cereale L.). J. Exp. Bot. 33, 261–268

    Google Scholar 

  23. Shewry, P.R.; Lew, E.J.-L.; Kasarda, D.D. (1981): Structural homology of storage proteins coded by the Hor-1 locus of barley. Planta 153, 246–253

    Google Scholar 

  24. Søgaard, B. (1974): The localization of Eceriferum loci in barley III. Three point tests of genes on chromosome 1 in barley. Hereditas 76, 41–48

    Google Scholar 

  25. Søgaard, B. (1977): The localization of Eceriferum loci in barley V. Three point tests of genes on chromosome 1 and 3 in barley. Carlsberg Res. Commun. 42, 67–75

    Google Scholar 

Download references

Author information

Affiliations

Authors

Additional information

Scientific Paper No. 6229. College of Agriculture Research Center, Washington State University, Pullman, Washington, Project Number 1006. This investigation supported in part by funds provided to Washington State University through the NIH Biomedical Research Support Grant

Communicated by D. von Wettstein

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Blake, T.K., Ullrich, S.E. & Nilan, R.A. Mapping of the Hor-3 locus encoding D hordein in Barley. Theoret. Appl. Genetics 63, 367–371 (1982). https://doi.org/10.1007/BF00303909

Download citation

Key words

  • Storage proteins
  • Hordein D
  • Three point test
  • Hordeum vulgare