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Histochemical studies on the effects of oxygen deprivation in the hair cells of Corti's organ

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Archiv für klinische und experimentelle Ohren-, Nasen- und Kehlkopfheilkunde Aims and scope Submit manuscript

Summary

  1. 1.

    Histochemical examinations can sensitively demonstrate functional changes in the hair cells.

  2. 2.

    When hair cells are made anoxic, the following three turning points occur in their intracytoplasmic metabolism.

  3. a)

    The first 10 min of anoxia produces a reversible dysfunction of aerobic decarboxylation.

  4. b)

    Anoxia of 30 min duration is critical in the partial recovery of disturbed cellular respiration.

  5. c)

    After 60 min of anoxia, aerobic respiration is irreversibly inhibited and is substituted for by anaerobic glycolysis.

  6. d)

    After 210 min of anoxia, anaerobic glycolysis also disappears.

  7. 3.

    The process of dissociation of cellular respiration and the relationship between aerobic and anaerobic glycolysis correlate well with the changes of the cochlear microphonics in anoxia.

Zusammenfassung

Durch histochemische Untersuchung des Corti'schen Organs von neugeborenen Hunden wurde die Wirkung von Sauerstoffmangel auf LDH und SDH der Haarzellen untersucht.

Dabei zeigte sich, daß nach Anoxie von 10 min die Abnahme der SDH noch reversibel ist. Nach 30 min Anoxie kommt es nur teilweise zur Regeneration der SDH.

Nach 60 min Anoxie ist die SDH irreversiby nicht mehr nachzuweisen, statt dessen kommt es jetzt zu einer vermehrten LDH-Aktivität.

Nach 210 min Anoxie ist such die LDH nicht mehr nachweisbar.

Diese Resultate entsprechen den bekannten elektrophysiologischen Untersuchungsbefunden nach Sauerstoffmangel.

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Authors and Affiliations

  1. Department of Otolaryngology, Faculty of Medicine, Kyoto University, Japan

    T. Yamashita, Y. Okano & H. Iwai

  2. Universitäts-HNO-Klinik, Ludwig Rehn-Straße 14, D-6000, Frankfurt a. M., Germany

    T. Yamashita

Authors
  1. T. Yamashita
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  2. Y. Okano
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  3. H. Iwai
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Yamashita, T., Okano, Y. & Iwai, H. Histochemical studies on the effects of oxygen deprivation in the hair cells of Corti's organ. Arch. Klin. Exp. Ohr.-, Nas.- U. Kehlk. Heilk. 204, 297–307 (1973). https://doi.org/10.1007/BF00303738

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  • DOI: https://doi.org/10.1007/BF00303738

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