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Histochemie

, Volume 35, Issue 1, pp 81–91 | Cite as

Fine structural localization of acid phosphomonoesterase in the osteoblasts and osteocytes of fracture callus

  • Gustav Göthlin
  • Jan L. E. Ericsson
Article

Summary

The fine structural localization of acid phosphatase was studied in osteoblasts and osteocytes of fracture callus in the rat using glutaraldehyde-fixed EDTA-decalcified, dimethylsulfoxide-treated tissues incubated in a modified Gomori lead salt medium. The results showed that enzyme was not only localized in conventional lysosomes but also in Golgi cisternae, Golgi associated vesicles, and — in the case of osteoblasts — GERL-like regions. The Golgi regions were large and abundant in osteoblasts and small and inconspicuous in osteocytes while lysosomes were of approximately equal size in the two cell types but appeared to be more concentrated in osteocytes. The results were discussed in relation to the possible role of lysosomes and lysosomal enzymes in osteocytic osteolysis and the functional diversity of the Golgi apparatus in osteogenic cells.

Keywords

Public Health Acid Phosphatase Functional Diversity Structural Localization Golgi Apparatus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baud, C.A.: The fine structure of normal and parathormone-treated bone cells. Proc. Fourth Europ. Symp. Calcified Tissues, Leiden, 1966 (eds. Gaillard, P. J., Van den Hooff, A., and Steendijk, R.). Int. Congress Series No 120, p. 4–6. Amsterdam: Excerpta Medica Foundation.Google Scholar
  2. Baud, C.A.: Submicroscopic structure and functional aspects of the osteocyte. Clin. Orthop. 56, 227–236 (1968).Google Scholar
  3. Belanger, L.F.: Osteolysis: an outlook on its mechanism and causation. In: The parathyroid glands, ultrastructure, secretion, and function (eds. Gaillard, P. J., Talmage, R. V., and Budy, A. M.), p. 137–143. Chicago: Chicago Univ. Press 1965.Google Scholar
  4. Belanger, L.F.: Osteocytic osteolysis. Calcif. Tiss. Res. 4, 1–12 (1969).Google Scholar
  5. Belanger, L.F.: Osteocytic resorption. In: The biochemistry and physiology of bone, 2nd ed., vol. III, Development and growth (ed. G.H. Bourne), p. 239–270. New York and London: Academic Press, 1971.Google Scholar
  6. Belanger, L.F., Belanger, C., Semba, T.: Technical approaches leading to the concept of osteocytic osteolysis. Clin. Orthop. 54, 187–196 (1967).Google Scholar
  7. Belanger, L.F., Migicovsky, B.B.: Histochemical evidence of proteolysis in bone: the influence of parathormone. J. Histochem. Cytochem. 11, 734–737 (1963).Google Scholar
  8. Belanger, L.F., Robichon, J., Migicovsky, B. B., Copp, D. H., Vincent, J.: Resorption without osteoclasts (osteolysis). In: Mechanism of hard tissue destruction (ed. R. F. Sognnaes), p. 531–556. Washington D. C.: Amer. Ass. Advance Sci. 1963.Google Scholar
  9. Belanger, L.F., Semba, T., Tolnai, S., Copp, D. H., Krook, L., Gries, C.: The two faces of resorption. In III European Symposium on Calcified Tissues (eds. H. Fleisch, H. J. J. Blackwood, and M. Owen), p. 1. Berlin-Heidelberg-New York: Springer 1966.Google Scholar
  10. Brunk, U.T., Ericsson, J.L.E.: The demonstration of acid phosphatase in in vitro cultured tissue cells. Studies on the significance of fixation, tonicity and permeability. Histochem. J. 4, 349–363 (1972).Google Scholar
  11. Cameron, D.A.: The fine structure of bone and calcified cartilage. Clin. Orthop. 26, 199–228 (1963).Google Scholar
  12. Cameron, D.A.: The Golgi apparatus in bone and cartilage cells. Clin. Orthop. 58, 191–211 (1968).Google Scholar
  13. Cameron, D.A.: The fine structure and function of bone cells. In: The biological basis of medicine (eds. E. E. Bittar and E. Bittar), vol. 3, p. 391–423. London and New York: Academic Press 1969.Google Scholar
  14. Carneiro, J. and Leblond, C.P.: Role of osteoblasts and odontoblasts in secreting the collagen of bone and dentin as shown by radioautography in mice given tritium-labelled glycine. Exp. Cell Bes. 18, 191–300 (1959).Google Scholar
  15. Doty, S. B., Schofield, B. H., Robinson, R. A.: The electron microscopic identification of acid phosphatase and adenosine triphosphatase in bone cells following parathyroid extract or thyrocalcitonin administration. In: Parathyroid hormone and thyrocalcitonin (calcitonin). Proceedings of the Third Parathyroid Conference, p. 169–181, Montreal, October 1967.Google Scholar
  16. Dudley, H.R., Spiro, D.: The fine structure of bone cells. J. Biophys. biochem. Cytol. 11, 627–649 (1961).Google Scholar
  17. Ericsson, J.L.E., Göthlin, G.: Preparation of calcified tissue for fine structural demonstration of acid phosphatase. J. Ultrastruct. Res. 38, 195 (1971).Google Scholar
  18. Göthlin, G.: Electron microscopic observations on fracture repair in the rat. Acta path. microbiol. scand. (1973) (in press).Google Scholar
  19. Göthlin, G., Ericsson, J.L.E.: Electron microscopic studies of cytoplasmic filaments and fibers in different cell types of fracture callus in the rat. Virchows Arch. Abt. B 6, 24–38 (1970).Google Scholar
  20. Göthlin, G., Ericsson, J.L.E.: Fine structural localization of acid phosphomonoesterase in the brush border region of osteoclasts. Histochemie 28, 337–344 (1971).Google Scholar
  21. Göthlin, G., Ericsson, J.L.E.: Observations on the mode of uptake of thorium dioxide paricles by osteoclasts in fracture callus. Calcif. Tiss. Res. 10, 216–222 (1972a).Google Scholar
  22. Göthlin, G., Ericsson, J. L. E.: Electron microscopic studies on the uptake and storage of thorium dioxide molecules in different cell types of fracture callus (1972b) (in manuscript).Google Scholar
  23. Helminen, H.J., Ericsson, J.L.E.: On the mechanism of lysosomal enzyme secretion. Electron microscopic and histochemical studies on epithelial cells of the rat's ventral prostate lobe. J. Ultrastruct. Res. 33, 528–549 (1970).Google Scholar
  24. Liu, T., Cameron, D.A.: Autoradiographic studies of collagen synthesis in bone. J. dent. Res. 44, 1191 (1965).Google Scholar
  25. Manning, J.P., Butler, M.C.: Simultaneous formalin fixation and EDTA decalcification, with carbonwax embedding for preservation of acid phosphatase. Stain Technol. 40, 7–12 (1965).Google Scholar
  26. Novikoff, A.B.: Enzyme localization and ultrastructure of neurons. In: The neuron (ed. H. Hyden), p. 255–318. Amsterdam-London-New York: Elsevier Publishing Company 1967.Google Scholar
  27. Recklinghausen, F. v.: Untersuchungen über Rachitis und Osteomalazie. Jena: Fischer 1910.Google Scholar
  28. Ross, R., Benditt, E.P.: Wound healing and collagen formation. V. Quantitative electron microscopic radioautographic observations of proline-H3 utilization by fibroblasts. J. Cell Biol. 27, 83–106 (1965).Google Scholar
  29. Ruyter, J.H.C.: Studies on an improved lead phosphate technique for the demonstration of non-specific acid phosphatase in nondeparaffinized organ and tissue sections. Histochemie 3, 521–537 (1964).Google Scholar
  30. Talmage, R.V.: A study of the effect of parathyroid hormone on bone remodeling and on calcium homeostasis. Clin. Orthop. 54, 163–173 (1967).Google Scholar
  31. Vaes, G.: The role of lysosomes and of their enzymes in the development of bone resorption induced by parathyroid hormone. In: Parathyroid hormone and thyreocalcitonin (eds. R. V. Talmage and L. F. Belanger), p. 318–328. Amsterdam: Excerpta Medica Found. 1968a.Google Scholar
  32. Vaes, G.: On the mechanisms of bone resorption. The action of parathyroid hormone on the excretion and synthesis of lysosomal hydrolytic enzymes and on the extracellular release of acid by bone cells. J. Cell Biol. 39, 676–697 (1968b).Google Scholar
  33. Vaes, G.: Lysosomes and the cellular physiology of bone resorption. Chap. 8 in: Lysosomes in biology and pathology (eds. J. T. Dingle and H. B. Fell), vol 1, p. 217–253. Amsterdam and London: North Holland Publishing Company 1969.Google Scholar
  34. Woods, J.F., Nichols, G., Jr.: Collagenolytic activity in rat bone cells: Characteristics and intracellular location. J. Cell Biol. 26, 747–757 (1965).Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Gustav Göthlin
    • 1
    • 2
  • Jan L. E. Ericsson
    • 1
    • 2
  1. 1.Department of Pathology at Sabbatsberg HospitalKarolinska Institutet Medical SchoolStockholmSweden
  2. 2.Department of Orthopedic Surgery at NorrbackainstitutetKarolinska Institutet Medical SchoolStockholmSweden

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