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Histochemie

, Volume 35, Issue 1, pp 75–80 | Cite as

The use of menadione as an intermediate hydrogen-carrier for measuring cytoplasmic dehydrogenating enzyme activities

  • J. Chayen
  • N. Loveridge
  • G. S. Ubhi
Article

Summary

Phenazine methosulphate is often used as an intermediate hydrogen-carrier in the demonstration of dehydrogenase activities. It cannot be used with adrenal tissue sections because it appears to have an inhibitory effect in this tissue.

The water-soluble menadione sodium-bisulphite salt has been shown to be quantitatively as efficient as phenazine methosulphate for pentose-shunt dehydrogenase activity in rat liver sections; at optimal concentrations it does not inhibit this activity in the adrenal.

Keywords

Public Health Enzyme Activity Tissue Section Optimal Concentration Dehydrogenase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Altman, F. P.: A comparison of dehydrogenase activities in tissue homogenates and tissue sections. Biochem. J. 114, 13–14P (1969a).Google Scholar
  2. Altman, F. P.: The use of eight different tetrazolium salts for a quantitative study of pentose shunt dehydrogenation. Histochemie 19, 362–374 (1969b).Google Scholar
  3. Altman, F. P.: Quantitative dehydrogenase histochemistry with special reference to the pentose shunt dehydrogenases. Progr. Histochem. Cytochem. 4, (4) 225–273 (1972).Google Scholar
  4. Chayen, J., Altman, F. P., Butcher, R. G.: The production and possible utilisation of reducing equivalents outside the mitochondria and the effects of certain drugs. In: Fundamentals of cell pharmacology, ed. S. Dikstein. Springfield, Illinois: Thomas 1972.Google Scholar
  5. Chayen, J., Bitensky, L., Butcher, R. G.: Practical histochemistry. London: John Wiley 1973.Google Scholar
  6. Chayen, J., Bitensky, L., Butcher, R. G., Poulter, L. W.: A guide to practical histochemistry. Edinburgh: Oliver and Boyd 1969.Google Scholar
  7. Chayen, J., Bitensky, L., Butcher, R. G., Poulter, L. W., Ubhi, G. S.: Methods for the direct measurement of anti-inflammatory action on human tissue maintained in vitro. Br. J. Derm. 82, Suppl. 6, 62–81 (1970).Google Scholar
  8. Gillette, J. R., Conney, A. H., Cosmides, G. H., Estabrook, R. W., Fouts, J. R., Mannering, G. J.: Microsomes and drug oxidations. New York: Academic Press 1969.Google Scholar
  9. Novikoff, A. B.: Electron transport enzymes: biochemical and tetrazolium staining methods. In: Histochemistry and cytochemistry, ed. by R. Wegmann, p. 465–481. Oxford: Pergamon Press 1963.Google Scholar
  10. Stuart, J., Bitensky, L., Chayen, J.: Quantitative enzyme cytochemistry of leukaemic cells. J. clin. Path. 22, 563–566 (1969).Google Scholar
  11. Stuart, J., Skowron, P. N.: A cytochemical study of marrow enzymes in megaloblastic anaemia. Brit. J. Haemat. 15, 443–454 (1968).Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • J. Chayen
    • 1
  • N. Loveridge
    • 1
  • G. S. Ubhi
    • 1
  1. 1.Division of Cellular BiologyThe Mathilda and Terence Kennedy Institute of RheumatologyLondonEngland

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