The use of menadione as an intermediate hydrogen-carrier for measuring cytoplasmic dehydrogenating enzyme activities
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Phenazine methosulphate is often used as an intermediate hydrogen-carrier in the demonstration of dehydrogenase activities. It cannot be used with adrenal tissue sections because it appears to have an inhibitory effect in this tissue.
The water-soluble menadione sodium-bisulphite salt has been shown to be quantitatively as efficient as phenazine methosulphate for pentose-shunt dehydrogenase activity in rat liver sections; at optimal concentrations it does not inhibit this activity in the adrenal.
KeywordsPublic Health Enzyme Activity Tissue Section Optimal Concentration Dehydrogenase Activity
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- Altman, F. P.: A comparison of dehydrogenase activities in tissue homogenates and tissue sections. Biochem. J. 114, 13–14P (1969a).Google Scholar
- Altman, F. P.: The use of eight different tetrazolium salts for a quantitative study of pentose shunt dehydrogenation. Histochemie 19, 362–374 (1969b).Google Scholar
- Altman, F. P.: Quantitative dehydrogenase histochemistry with special reference to the pentose shunt dehydrogenases. Progr. Histochem. Cytochem. 4, (4) 225–273 (1972).Google Scholar
- Chayen, J., Altman, F. P., Butcher, R. G.: The production and possible utilisation of reducing equivalents outside the mitochondria and the effects of certain drugs. In: Fundamentals of cell pharmacology, ed. S. Dikstein. Springfield, Illinois: Thomas 1972.Google Scholar
- Chayen, J., Bitensky, L., Butcher, R. G.: Practical histochemistry. London: John Wiley 1973.Google Scholar
- Chayen, J., Bitensky, L., Butcher, R. G., Poulter, L. W.: A guide to practical histochemistry. Edinburgh: Oliver and Boyd 1969.Google Scholar
- Chayen, J., Bitensky, L., Butcher, R. G., Poulter, L. W., Ubhi, G. S.: Methods for the direct measurement of anti-inflammatory action on human tissue maintained in vitro. Br. J. Derm. 82, Suppl. 6, 62–81 (1970).Google Scholar
- Gillette, J. R., Conney, A. H., Cosmides, G. H., Estabrook, R. W., Fouts, J. R., Mannering, G. J.: Microsomes and drug oxidations. New York: Academic Press 1969.Google Scholar
- Novikoff, A. B.: Electron transport enzymes: biochemical and tetrazolium staining methods. In: Histochemistry and cytochemistry, ed. by R. Wegmann, p. 465–481. Oxford: Pergamon Press 1963.Google Scholar
- Stuart, J., Bitensky, L., Chayen, J.: Quantitative enzyme cytochemistry of leukaemic cells. J. clin. Path. 22, 563–566 (1969).Google Scholar
- Stuart, J., Skowron, P. N.: A cytochemical study of marrow enzymes in megaloblastic anaemia. Brit. J. Haemat. 15, 443–454 (1968).Google Scholar