, Volume 35, Issue 1, pp 75–80 | Cite as

The use of menadione as an intermediate hydrogen-carrier for measuring cytoplasmic dehydrogenating enzyme activities

  • J. Chayen
  • N. Loveridge
  • G. S. Ubhi


Phenazine methosulphate is often used as an intermediate hydrogen-carrier in the demonstration of dehydrogenase activities. It cannot be used with adrenal tissue sections because it appears to have an inhibitory effect in this tissue.

The water-soluble menadione sodium-bisulphite salt has been shown to be quantitatively as efficient as phenazine methosulphate for pentose-shunt dehydrogenase activity in rat liver sections; at optimal concentrations it does not inhibit this activity in the adrenal.


Public Health Enzyme Activity Tissue Section Optimal Concentration Dehydrogenase Activity 
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • J. Chayen
    • 1
  • N. Loveridge
    • 1
  • G. S. Ubhi
    • 1
  1. 1.Division of Cellular BiologyThe Mathilda and Terence Kennedy Institute of RheumatologyLondonEngland

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