Advertisement

Histochemie

, Volume 35, Issue 1, pp 39–50 | Cite as

Acid phosphatase in the Golgi apparatus of cells forming extracellular matrix of hard tissues

  • Jacob S. Hanker
  • Andrew D. Dixon
  • Gary R. Smiley
Article

Summary

Histochemical studies using cryostat sections of fixed rodent fetal and newborn tissues indicated that acid phosphatase (APase) staining of the Golgi apparatus (GA) of cells secreting matrix for hard tissue formation was a general phenomenon. The enzyme was chiefly observed in the GA of tall secretory ameloblasts involved in enamel formation and in the GA of odontoblasts forming dentine; lysosome-like granules reactive for this enzyme were also observed in these cells. Activity was also intense in the GA and lysosomes of osteoblasts involved in intramembranous and endochondral bone formation.

High levels of APase in the GA of extracellular matrix-forming cells appeared to correlate with secretory activity. The GA of most other cells, even chondroblasts forming cartilage matrix, had much less marked APase activity. Contrary to previous suggestions, it appears that APase may have a more direct role in osteogenesis than the osteolytic or resorptive action usually cited.

Keywords

Bone Formation Acid Phosphatase Golgi Apparatus Tissue Formation Hard Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Beams, H.W., King, R.L.: The Golgi apparatus in the developing tooth, with special reference to polarity. Anat. Rec. 57, 29–39 (1933).Google Scholar
  2. Burstone, M.S.: Hydrolytic enzymes in dentinogenesis and osteogenesis. In: Calcification in biological systems, p. 217–243 (Sognnaes, R.F., ed.). Washington, D.C.: American Association for the Advancement of Science 1960.Google Scholar
  3. Cabrini, R.L.: Histochemistry of ossification. Int. Rev. Cytol. 11, 283–306 (1961).Google Scholar
  4. Cameron, D.A.: The fine structure of bone and calcified cartilage. Clin. Orthop. 26, 199–228 (1963).Google Scholar
  5. Cameron, D.A.: The Golgi apparatus in bone and cartilage cells. Clin. Orthop. 58, 191–211 (1968).Google Scholar
  6. Carneiro, J., Leblond, C.P.: Role of osteoblasts and odontoblasts in secreting the collagen of bone and dentin as shown by radioautography in mice given tritium-labeled glycine. Exp. Cell Res. 18, 291–300 (1959).Google Scholar
  7. Cederberg, I.: The occurrence of alkaline and acid phosphomonoesterases in the tooth germ of albino rats. Acta anat. (Basel) 12, 334–340 (1951).Google Scholar
  8. Cohn, S.A.: Development of molar teeth in the albino mouse. Amer. J. Anat. 101, 295–319 (1957).Google Scholar
  9. Dauwalder, M., Whaley, M.G., Kephart, J. E.: Phosphatases and differentiation of the Golgi apparatus. J. Cell Sci. 4, 455–497 (1969).Google Scholar
  10. Deane, H.W., Dempsey, E.W.: The localization of phosphatases in the Golgi region of intestinal and other epithelial cells. Anat. Rec. 93, 401–417 (1945).Google Scholar
  11. Dixon, T.F., Perkins, H.R.: The chemistry of calcification. In: The biochemistry and physiology of bone, p. 287–307 (Bourne, G.H., Ed.). New York: Academic Press 1956.Google Scholar
  12. Elftman, H.: A direct silver method for the Golgi apparatus. Stain Technol. 27, 47–52 (1952).Google Scholar
  13. Fell, H.B., Robinson, R.: The development and phosphatase activity in vivo and in vitro of the mandibular skeletal tissue of the embryonic fowl. Biochem. J. 24, 1905–1941 (1930).Google Scholar
  14. Fullmer, H.M.: The histochemistry of the connective tissues. Int. Rev. Conn. Tiss. Res. 3, 1–76 (1965).Google Scholar
  15. Garant, P.S., Nalbandian, J.: Observations on the ultrastructure of ameloblasts with special reference to the Golgi complex and related components. J. Ultrastruct. Res. 23, 427–443, (1968).Google Scholar
  16. Gardner, E.: Osteogenesis in the human embryo and fetus. In: The biochemistry and physiology of bone, 2nd ed., vol. 3, p. 77–118 (Bourne, G.H., ed.). New York: Academic Press 1971.Google Scholar
  17. Godman, G.C., Lane, N.: On the site of sulfation in the chondrocyte. J. Cell Biol. 21, 353–366 (1964).Google Scholar
  18. Godman, G.C., Porter, K.R.: Chondrogenesis, studied with the electron microscope. J. biophys. biochem. Cytol. 8, 719–760 (1960).Google Scholar
  19. Goldfischer, S., Essner, E., Novikoff, A. B.: The localization of phosphatase activities at the level of ultrastructure. J. Histochem. Cytochem. 12, 72–95 (1964).Google Scholar
  20. Gomori, G.: Calcification and phosphatase. Amer. J. Path. 19, 197–207 (1943).Google Scholar
  21. Hammarström, L.E., Hanker, J.S., Toverud, S.U.: Cellular differences in acid phosphatase isoenzymes in bone and teeth. Clin. Orthop. 78, 151–167 (1971).Google Scholar
  22. Hanker, J.S., Hammarström, L.E., Toverud, S.U., Yates, P.E.: The formaldehyde-sensitivity of acid phosphatases involved in osteogenesis and odontogenesis in the rat. Arch. oral Biol. 17, 503–509 (1972).Google Scholar
  23. Hanker, J.S., Kasler, F., Bloom, M.G., Copeland, J.S., Seligman, A.M.: Coordination polymers of osmium: The nature of osmium black. Science 156, 1737–1738 (1967).Google Scholar
  24. Hanker, J.S., Seaman, A.R., Weiss, L.P., Ueno, H., Bergman, R.A., Seligman, A.M.: Osmiophilic reagents: New cytochemical principle for light and electron microscopy. Science 146, 1039–1043 (1964).Google Scholar
  25. Hanker, J.S., Yates, P.E., Clapp, D.H., Anderson, W.A.: New methods for the demonstration of lysosomal hydrolases by the formation of osmium blacks. Histochemie 30, 201–214 (1972).Google Scholar
  26. Johnson, M.L.: The time and order of appearance of ossification centers in the albino mouse. Amer. J. Anat. 52, 241–271 (1933).Google Scholar
  27. Johnson, P.L., Bevelander, G.: The localization and interrelation of nucleic acids and alkaline phosphatase in the developing tooth. J. dent. Res. 33, 128–135 (1954).Google Scholar
  28. Kallenbach, E., Sandborn, E., Warshawsky, H.: The Golgi apparatus of the ameloblast at the stage of enamel matrix formation. J. Cell Biol. 16, 629–632 (1963).Google Scholar
  29. Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137 A (1965).Google Scholar
  30. Katchburian, E., Holt, S.J.: Role of lysosomes in amelogenesis. Nature (Lond.) 223, 1367–1368 (1969).Google Scholar
  31. Katchburian, E., Katchburian, A.V., Pearse, A. G. E.: Histochemistry of lysosomal enzymes in developing teeth of albino rats. J. Anat. (Lond.) 101, 783–792 (1967).Google Scholar
  32. Leonard, E.P., Provenza, D.V.: Histochemical observations on the phosphatase activity of the developing dental primordia in the Swiss albino mouse as detected by azo dye and osmium-capture methods. Arch. oral Biol. 15, 635–643 (1970).Google Scholar
  33. Nassonow, D.N.: Das Golgische Binnenetz und seine Beziehungen zu der Sekretion. Morphologische und experimentelle Untersuchungen an einigen Säugetierdrüsen. Arch. mikr. Anat. 100, 433–472 (1924).Google Scholar
  34. Neutra, M., Leblond, C.P.: Radioautographic comparison of the uptake of galactose-H3 and glucose-H3 in the Golgi region of various cells secreting glycoproteins or mucopolysaccharides. J. Cell Biol. 30, 137–150 (1966).Google Scholar
  35. Northcote, D.H.: The Golgi apparatus. Endeavour 30, 26–33 (1971).Google Scholar
  36. Novikoff, A.B., Goldfischer, S.: Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness for cytological studies. Proc. nat. Acad. Sci. (Wash.) 47, 802–810 (1961).Google Scholar
  37. Pritchard, J.J.: A cytological and histochemical study of bone and cartilage formation in the rat. J. Anat (Lond.) 86, 259–277 (1952).Google Scholar
  38. Radden, B.G., Fullmer, H.M.: Morphological and histochemical studies of bone repair in the rat. Arch. oral Biol. 14, 1243–1252 (1969).Google Scholar
  39. Reith, E.J.: The stages of amelogenesis as observed in molar teeth of young rats. J. Ultrastruct. Res. 30, 111–151 (1970).Google Scholar
  40. Rose, G.R.: The Golgi complex of living osteoblasts. J. biophys. biochem. Cytol. 9, 463–478 (1961).Google Scholar
  41. Seligman, A.M., Hanker, J.S., Wasserkrug, H., Dmochowski, H., Katzoff, L.: Histochemical demonstration of some oxidized macromolecules with thiocarbohydrazide and osmium tetroxide. J. Histochem. Cytochem. 13, 629–639 (1965).Google Scholar
  42. Seligman, A.M., Wasserkrug, H.L., Deb, C., Hanker, J. S.: Osmium-containing compounds with multiple basic or acidic groups as stains for ultrastructure. J. Histochem. Cytochem. 16, 87–101 (1968).Google Scholar
  43. Smith, R.E., Farquhar, M.G.: Lysosome function in the regulation of the secretory process in cells of the anterior pituitary gland. J. Cell Biol. 31, 319–347 (1966).Google Scholar
  44. Sobel, H.J.: The localization of acid phosphatase activity in rat pituitary and thyroid glands and its relation to secretory activity. Endocrinology 68, 801–807 (1961).Google Scholar
  45. Sobel, H.J., Avrin, E.: The localization of acid phosphatase activity in rat pancreatic acinar cells: a light and electron microscopic study. J. Histochem. Cytochem. 13, 301–303 (1965).Google Scholar
  46. Weinstock, A., Leblond, C.P.: Elaboration of the matrix glycoprotein of enamel by the secretory ameloblasts of the rat incisor as revealed by radioautography. J. Cell Biol. 51, 26–51 (1971).Google Scholar
  47. Wirtschafter, Z.T.: The genesis of the mouse skeleton: A laboratory atlas, p. 5. Springfield, Illinois: C. C. Thomas 1960.Google Scholar
  48. Wergedal, J.E., Baylink, D.J.: Distribution of acid and alkaline phosphatase activity in undemineralized sections of rat tibial diaphysis. J. Histochem. Cytochem. 17, 799–806 (1969).Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Jacob S. Hanker
    • 1
  • Andrew D. Dixon
    • 1
  • Gary R. Smiley
    • 1
  1. 1.Dental Research Center, School of DentistryUniversity of North CarolinaChapel HillUSA

Personalised recommendations