, Volume 35, Issue 1, pp 39–50 | Cite as

Acid phosphatase in the Golgi apparatus of cells forming extracellular matrix of hard tissues

  • Jacob S. Hanker
  • Andrew D. Dixon
  • Gary R. Smiley


Histochemical studies using cryostat sections of fixed rodent fetal and newborn tissues indicated that acid phosphatase (APase) staining of the Golgi apparatus (GA) of cells secreting matrix for hard tissue formation was a general phenomenon. The enzyme was chiefly observed in the GA of tall secretory ameloblasts involved in enamel formation and in the GA of odontoblasts forming dentine; lysosome-like granules reactive for this enzyme were also observed in these cells. Activity was also intense in the GA and lysosomes of osteoblasts involved in intramembranous and endochondral bone formation.

High levels of APase in the GA of extracellular matrix-forming cells appeared to correlate with secretory activity. The GA of most other cells, even chondroblasts forming cartilage matrix, had much less marked APase activity. Contrary to previous suggestions, it appears that APase may have a more direct role in osteogenesis than the osteolytic or resorptive action usually cited.


Bone Formation Acid Phosphatase Golgi Apparatus Tissue Formation Hard Tissue 
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Jacob S. Hanker
    • 1
  • Andrew D. Dixon
    • 1
  • Gary R. Smiley
    • 1
  1. 1.Dental Research Center, School of DentistryUniversity of North CarolinaChapel HillUSA

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