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Histochemie

, Volume 35, Issue 1, pp 31–38 | Cite as

Application of the glyoxylic acid method to Vibratome sections for the improved visualization of central catecholamine neurons

  • Olle Lindvall
  • Anders Björklund
  • Tomas Hökfelt
  • Åke Ljungdahl
Article

Summary

The glyoxylic acid fluorescence histochemical method for the visualization of neuronal monoamines has been applied to fresh or glyoxylic acid-perfused brain tissue, sectioned with the Vibratome intrument. This technique demonstrates the central noradrenaline and dopamine neuron systems with a sensitivity and richness in details that is superior to the standard Falck-Hillarp formaldehyde method, as observed in the following three ways: First, the entire axon, including the non-terminal portions, became fluorescent; second, more extensive terminal systems were detected in certain brain regions ; third, due to the absence of diffusion, the delicate dopamine-containing fibres in e. g. the caudate nucleus and the median eminence had a distinct fluorescence. It is concluded that the glyoxylic acid method applied to Vibratome sections should be ideal for precise and detailed neuroanatomical studies on central catecholamine neuron systems.

Keywords

Dopamine Monoamine Caudate Nucleus Dopamine Neuron Neuron System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Olle Lindvall
    • 1
    • 2
  • Anders Björklund
    • 1
    • 2
  • Tomas Hökfelt
    • 1
    • 2
  • Åke Ljungdahl
    • 1
    • 2
  1. 1.Department of HistologyUniversity of LundLund
  2. 2.Department of HistologyKarolinska InstitutetStockholmSweden

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