, Volume 35, Issue 1, pp 1–10 | Cite as

Polysaccharides associated with chromosomes and their behavior in the cell cycle

  • Takayuki Ohnishi
  • Kiyotaka Yamamoto
  • Hiroshi Terayama


The interphase nuclei, especially of the latest stages (G2 or early prophase), in the mouse and rat livers were stained blue in the histochemical demonstration of acidic polysaccharide according to the method of Mowry, while the mitotic chromosomes (meta-, ana- and telophase) in the livers, sea urchin embryos as well as root tips of broad beans were stained red, suggesting the presence of neutral polysaccharide. The giant polytenic interphase chromosome of the salivary gland of Chironomus larvae was stained blue in the puffing and nucleolar regions while stained red in the condensed part of the chromosome. 3H-Glucosamine as well as 3H-glucose incorporations into the regenerating rat liver nuclei reached a peak at 30 h after partial hepatectomy when the highest mitosis is seen. These results suggest that the nuclear acid mucopolysaccharide present in the swollen chromosomes may be converted to or replaced with the neutral polysaccharide in the condensed chromosomes such as mitotic chromosomes or polytenic giant interphase chromosomes.


Mitotic Chromosome Broad Bean Mucopolysaccharide Acid Mucopolysaccharide Early Prophase 
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Takayuki Ohnishi
    • 1
  • Kiyotaka Yamamoto
    • 2
  • Hiroshi Terayama
    • 2
  1. 1.Department of Biology, Faculty of ScienceTokyo Institute of TechnologyTokyo
  2. 2.Zoological Institute, Faculty of ScienceUniversity of TokyoTokyo

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