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The effect of the r a and r b loci on the lipid content of the seed of Pisum sativum


The lipid content of seed from a set of isogenic lines for the R a : r a locus has been determined; the results show that this locus as well as affecting the starch, sugar and storage protein content and composition, also has a marked effect on the lipid content of the seed. Genotypes having different combinations of alleles at the r a and r b loci have also been examined; an r a r a r b r b , genotype had 5.57% purified total lipid in its seed — a more than 2-fold increase over that found in the round-seeded varieties (R a R a R b R b ) usually grown for the dry sed crop.

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  • Blixt, S. (1972): Mutation genetics in Pisum. Agr. Hort. Genet. 30, 1–293

    Google Scholar 

  • Colonna, P.; Gallant, D.; Mercier, C. (1980): Pisum sativum and Vicia faba carbohydrates: studies of fractions obtained after dry and wet protein extraction processes. J. Food Sci. 45, 1629–1636

    Google Scholar 

  • Davies, D.R. (1980): The r a locus and legumin synthesis in Pisum sativum. Biochem. Genet. 18, 1207–1219

    Google Scholar 

  • Downey, R.K. (1963): Breeding for oil quality in rapeseed. Can. Food Ind. 34, 34

    Google Scholar 

  • Folch, J.; Lees, M.; Sloane Stanley, G.H. (1957): A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226, 497–509

    Google Scholar 

  • Haydar, M.; Hadziyev, D. (1973): Pea lipids and their oxidation on carbohydrate and protein matrices. J. Food Sci. 38, 772–778

    Google Scholar 

  • Horowitz, J. (1982): Ultrastructural and biochemical studies of storage proteins in Pisum. Ph.D. Thesis. University of East Anglia

  • Ingversen, J.; Køie, B.; Doll, H. (1973): Induced seed protein mutant of barley. Experientia 29, 1151

    Google Scholar 

  • Kooistra, E. (1962): On the differences between smooth and three types of wrinkled peas. Euphytica 11, 357–373

    Google Scholar 

  • Ladd, S.L.; Knowles, P.F. (1970): Inheritance of stearic acid in the seed of Safflower. Crop Sci. 10, 525

    Google Scholar 

  • Mertz, E.T.; Bates, L.S.; Nelson, O.E. (1964): Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145, 279

    Google Scholar 

  • Miflin, B.J.; Shewry, P.R. (1979): The synthesis of proteins in normal and high lysine barley seeds. In: Recent advances in the biochemistry of cereals, (eds. Laidman, D.; Wyn Jones, R.G.), pp. 239–273. New York: Acad. Press

    Google Scholar 

  • Munck, L.; Karlson, K.E.; Hagberg, A.; Eggum, B.O. (1970): Gene for improved nutritional value in barley seed protein. Science 168, 985–987

    Google Scholar 

  • Nelson, O.E. (1981): Genetic control of polysaccharide and storage protein synthesis in the endosperms of barley, maize, and sorghum. Chapter 2. Adv. Cereal Sci. Technol. III, 41–71

    Google Scholar 

  • Nelson, O.E.; Mertz, E.T.; Bates, L.S. (1965): Second mutant gene affecting the amino acid pattern of maize endosperm proteins. Science 150, 1469

    Google Scholar 

  • Rouser, G.; Kritchevsky, G.; Simon, G.; Nelson, G.J. (1967): Qualitative analysis of brain and spinach leaf lipids employing silicic acid column chromatography and acetone for elution of glycolipids. Lipids 2, 37–40

    Google Scholar 

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Communicated by R. Riley

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Coxon, D.T., Davies, D.R. The effect of the r a and r b loci on the lipid content of the seed of Pisum sativum . Theoret. Appl. Genetics 64, 47–50 (1982).

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Key words

  • Lipid content
  • Storage protein
  • Legumeseeds
  • Genetic control
  • Pea
  • Pisum sativum