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Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720

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Abstract

A 21-kDa novel polypeptide which possesses characteristics normally considered to be diagnostic of the calmodulin present in eukaryotic cells was isolated from the cyanobacterium Nostoc sp. PCC 6720. The major technique employed in the isolation of the polypeptide was ion-exchange chromatography on a Mono Q column. The 21-kDa polypeptide was shown: to activate pea NAD kinase in vitro, in a Ca2+ requiring reaction; to react with polyclonal antibodies raised against spinach calmodulin, but not with those raised against bovine brain calmodulin; and to exhibit a Ca2+ dependent shift in migration during SDS-PAGE.

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Abbreviations

ATCC:

American Type Culture Collection

DCPIP:

2,6-dichlorophenylindophenol

PBS:

Phosphate buffered saline

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Authors and Affiliations

  1. Institute of Environmental and Biological Sciences, Division of Biological Sciences, Lancaster University, LA1 4YQ, Lancaster, UK

    Leonzio A. Onek, Peter J. Lea & R. John Smith

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  1. Leonzio A. Onek
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  2. Peter J. Lea
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  3. R. John Smith
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Onek, L.A., Lea, P.J. & Smith, R.J. Isolation and characterization of a calmodulin-like protein from the cyanobacterium Nostoc sp. PCC 6720. Arch. Microbiol. 161, 352–358 (1994). https://doi.org/10.1007/BF00303592

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  • DOI: https://doi.org/10.1007/BF00303592

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