Summary
7-Hydroxymethyl-12-methylbenz(a)anthracene and 12-hydroxymethyl-7-methylbenz(a)anthracene were detected together with small amounts of 7,12-dihydroxy-methylbenz(a)anthracene and 8,9-dihydro-8,9-dihydroxy-7,12-dimethylbenz(a)anthracene. When homogenates of the stomachs of mice that had been pretreated with 3-methylchol-anthrene were incubated with 7,12-dimethylbenz(a)anthracene, no significant increases in the amounts of the metabolites formed were found. There were increases in the amounts of the 8,9-dihydrodiol formed when homogenates of the small intestines of mice that had been similarly treated were incubated with the hydrocarbons. The levels of “aryl hydrocarbon hydroxylase” were raised in homogenates of the small intestine of treated animals as compared with the levels of the enzyme in homogenates from normal animals.
Zusammenfassung
Gefunden wurden 7-Hydroxymethyl-12-methylben(a)anthracene und 12-hydroxymethyl-7-methylbenz(a)anthracen zusammen mit kleinen Mengen von 7,12-Dihydroxymethylbenz(a)anthracen und 8,9-Dihydro-8,9-dihydroxy-7,12-dimethylbenz(a)-anthracen. Wenn Magenhomogenate von Mäusen vorher mit 3-Methylcholanthren behandelt waren, mit 7,12-Dimethylbenz(a)anthracen inkubiert wurden, konnte kein signifikanter Anstieg der Metaboliten gefunden werden. Wohl aber fand sich ein Anstieg am Gehalt von 8,9-Dihydrodiol, wenn Homogenate des Dünndarms mit den Kohlenwasserstoffen inkubiert wurden. Das Niveau der „Aryl Hydrocarbon Hydroxylase” war erhöht in Homogenanten des Dünndarms von vorbehandelten Tieren im Vergleich mit des Niveau des Enzyms in Homogenaten von Normaltieren.
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This work was supported by a grant (to A.G.) from the Royal Society, London.
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Gentil, A., Sims, P. The metabolism of 7,12-dimethylbenz (a) anthracene by homogenates of the stomach and small intestine of mice. Z. Krebsforsch. 76, 223–230 (1971). https://doi.org/10.1007/BF00303567
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DOI: https://doi.org/10.1007/BF00303567