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Structure of melon rDNA and nucleotide sequence of the 17–25S spacer region

Theoretical and Applied Genetics volume 76, pages 673–680 (1988)Cite this article

Summary

Restriction enzyme and hybridization analysis of melon nuclear DNA suggests a homogenous rDNA population with a repeat unit of 10.2 kb. Several full length Hind III rDNA repeat units were cloned and one of these is described in detail. The regions coding for 25S, 17S and 5.8S rRNAs were located by crossed-contact hybridization and R-loop mapping. Introns were not observed. The nucleotide sequence of the internal transcribed spacer and flanking regions was determined and compared with the corresponding region from rice rDNA by dot matrix analysis. In addition, the extent of gross sequence homology between cloned melon and pea rDNA units was determined by heteroduplex mapping.

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Author notes

  1. T. A. Kavanagh

    Present address: Department of Genetics, Trinity College, Dublin 2, Ireland

Affiliations

  1. Department of Botany, University College, 4, Belfield, Dublin, Ireland

    T. A. Kavanagh & J. N. Timmis

  2. Department of Genetics, University of Adelaide, 498, 5001, Adelaide, South Australia

    J. N. Timmis

Authors
  1. T. A. Kavanagh
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  2. J. N. Timmis
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Communicated by H. F. Linskens

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Kavanagh, T.A., Timmis, J.N. Structure of melon rDNA and nucleotide sequence of the 17–25S spacer region. Theoret. Appl. Genetics 76, 673–680 (1988). https://doi.org/10.1007/BF00303512

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  • DOI: https://doi.org/10.1007/BF00303512

Key words

  • Melon
  • Ribosomal DNA
  • R-loops
  • Heteroduplex analysis
  • 17-25S spacer sequence