Skip to main content
SpringerLink
Log in

Histochemical tests on lipids in the oral and skin epithelia in the mouse

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

The present study concerns the application of a new tissue transport medium, Histocon, in lipid histochemical analysis of biopsies. It was generally found that this medium visibly retained more lipids quantitatively than did a fixation of the tissues. The lipid “preserving” capacity of Histocon seemed to be superior even to the use of fresh frozen specimens. These beneficial effects may be due to membrane stabilizing factors in the transport medium.

Two types of lipids were usually found when comparing oral mucosa with epidermis. The mucosa was characterized chiefly by phospholipids with an increasing concentration gradient towards the stratum granulosum. The skin showed, in addition to the similar phospholipid gradient, also the presence of unsaturated hydrophobic lipids in the stratum corneum and granulosum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adams, C. W. M.: A histochemical method for the simultaneous demonstration of normal and degenerating myelin. J. Path. Bact. 77, 648–650 (1959).

    Google Scholar 

  • Adams, C. W. M.: Neurohistochemistry, p. 6–66. Amsterdam: Elsevier 1965.

    Google Scholar 

  • Adams, C. W. M., Bayliss, O. B., Ibrahim, M. Z. M.: Modifications to histochemical methods for phosphoglyceride and cerebroside. J. Histochem. Cytochem. 11, 560–561 (1963).

    Google Scholar 

  • Anneroth, G., Ivemark, B. I.: Histochemical localization of lipids in the human gingival epithelium and their relation to the dry mass concentration. Odont. Bevy 22, 7–18 (1971).

    Google Scholar 

  • Barka, T., Anderson, P. J.: Histochemistry: Theory, practice and bibliography. Hoeber Med. Div., New York: Harper & Row 1965.

    Google Scholar 

  • Edgar, G. W. F., Donker, C. H. M.: Influence of lipid solvents on sphingolipids (sphingomyelins, cerebrosides, gangliosides) in tissue sections. Acta neurol. belg. 5, 451–461 (1957).

    Google Scholar 

  • From, S. H.: Quantitative histochemistry of lactate dehydrogenase (LDH) in mouse submandibular gland (Abstract). J. dent. Res. 50, 719 (1971).

    Google Scholar 

  • Fullmer, H. M.: Enzymes in mineralized tissues. Clin. Orthop. 48, 285–295 (1966).

    Google Scholar 

  • Hall, J. D., Crane, F. L.: Disruption of mitochondrial membrane by acetone extraction. Biochim. biophys. Acta (Amst.) 241, 682–686 (1971).

    Google Scholar 

  • Heslinga, F. J. M., Deierkauf, F. A.: The action of formaldehyde solutions on human brain lipids. J. Histochem. Cytochem. 10, 704–709 (1962).

    Google Scholar 

  • Heyden, G., Arwill, T., Gisslén, H.: Histochemical studies on lichen planus. Oral Surg. (in press) (1972).

  • Heyden, G., Arwill, T., Lilja, J., Magnusson, B. C.: Chlorhexidine solutions in histological and histochemical techniques. J. oral Path. 1, 12–21 (1972).

    Google Scholar 

  • Heyden, G., Magnusson, B. C., Arwill, T.: Histochemical study of rhodamine B affinity in oral tissues of the mouse. Scand. J. dent. Res. 80, 40–46 (1972).

    Google Scholar 

  • Hirsch, T., Pfeiffer, J.: A histochemical study of the prelipid and metachromatic degenerative products in leucodystrophy. In: Cerebral lipidoses, p. 68–76, ed. J. N. Cunnings. Oxford, 1957.

  • Holczinger, L.: Histochemischer Nachweis freier Fettsäuren. Acta histochem. (Jena) 8, 167–175 (1959).

    Google Scholar 

  • Hopwood, D.: Fixatives and fixation: a rewiew. Histochem. J. 1, 323–360 (1969).

    Google Scholar 

  • Irving, J. T., Wuthier, R. E.: Histochemistry and biochemistry of calcification with special reference to the role of lipids. Clin. Orthop. 56, 237–259 (1968).

    Google Scholar 

  • Jones, D., Gresham, A.: Reaction of formaldehyde with unsaturated fatty acids during histological fixation. Nature (Lond.) 210, 1386–1388 (1966).

    Google Scholar 

  • Liisberg, M. F.: Rhodamine B as an extremely specific stain for cornification. Acta anat. (Basel) 69, 52–57 (1968).

    Google Scholar 

  • Lillie, R. D.: Histopathologic technic and practical histochemistry, 3rd. ed. New York: Blakiston Div. McGraw Hill Book Co. 1965.

    Google Scholar 

  • Lison, L., Dagnelie, J.: Méthodes nouvelles de coloration de la myéline. Bull. Histol. Appl. 12, 85–91 (1935).

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry theoretical and applied, vol. 1, 3 rd. ed. London: Churchill 1968.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oral Histopathology, Faculty of Odontology, University of Göteborg, Sweden

    Bengt C. Magnusson, Guy Heyden & Tore Arwill

Authors
  1. Bengt C. Magnusson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guy Heyden
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tore Arwill
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported in part by the Swedish Medical Research Council, grant no. K 73-24 X-3340-02 B.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Magnusson, B.C., Heyden, G. & Arwill, T. Histochemical tests on lipids in the oral and skin epithelia in the mouse. Histochemie 34, 249–256 (1973). https://doi.org/10.1007/BF00303441

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00303441

Keywords

Search

Navigation