Abstract
To determine the relative potency of synthetic glucocorticoids, glucocorticoid receptor expressing cells were transfected with a hormone-inducible reporter gene, and were cultured in the presence of various glucocorticoid ligands. Hormonal inducbility was determined by means of a chloramphenicol acetyltransferase assay. Dexamethasone and prednisolone, as well as cortisol, induced the expression of the reporter gene in a dose-dependent fashion. The relative potency of each ligand was in this order when inducibility was quantitatively assessed. In conclusion, the transcription assay described here may be a convenient and alternative method to evaluate the relative potency of given glucocorticoids.
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References
Tyrrell JB, Baxter JD (1981) Glucocorticoid therapy. In: Felig P, Baxter JD, Broadus AE, Frohman LA (eds) Endocrinology and metabolism. McGraw-Hill, New York, pp 599–624
Evans RM (1988) The steroid and thyroid receptor superfamily. Science 241:889–895
Beato M (1989) Gene regulation by steroid hormones. Cell 56:335–344
Tolksdorf S (1950) Laboratory evaluation of anti-inflammatory steroids. Ann NY Acad Sci 82:829–835
Axelrod L (1976) Glucocorticoid therapy. Medicine (Baltimore) 55:39–65
Behrens TW, Goodwin JS (1989) Glucocorticoids. In: McCarty DJ (ed) Arthritis and allied conditions, 11th edn. Lea & Febiger, Philladelphia, pp 604–621
Alksnis M, Barkhem T, Strömstedt P-E, Ahola H, Kutoh E, Gustafsson J-Å, Poellinger L, Nilsson S (1991) High level expression of functional full length and truncated glucocorticoid receptor in Chinese hamster ovary cells. J Biol Chem 266:10078–10085
Tanaka H, Makino I (1992) Ursodeoxycholic acid-dependent activation of the glucocorticoid receptor. Biochem Biophys Res Commun 188:942–948
Tanaka H, Fukawa E, Hirano H, Makino Y, Aoki A, Morikawa Y, Takiyama Y, Tani T, Makino I (1992) In vitro monitoring activation by the ligands and specific DNA-binding of the glucocorticoid receptors. Endocrinol Jpn 39:477–483
An G, Hidaka K, Siminovitch L (1982) Expression of bacterial β-galactosidase in mammalian cells. Mol Cell Biol 2:1628–1632
Kimberley RP (1990) Steroids. Curr Opin Rheumatol 2:510–516
Akerblom IE, Mellon PL (1991) Repression of gene expression by steroid and thyroid hormones. In: Parker MG (ed) Nuclear hormone receptros. Academic Press, London, pp 175–196
Gustavson LE, Benet LZ (1985) Pharmacokinetics of natural and synthetic glucocorticoids. In: Anderson DC, Winter JSD (eds) Adrenal cortex. Butterworths, London, pp 235–281
Meikle AW, Tyler FH (1977) Potency and duration of action of glucocorticoids. Am J Med 63:200–207
Ballard PL, Carter JP, Graham BS, Baxter JD (1975) A radioreceptor assay for evaluation of the plasma glucocorticoid activity of natual and synthetic steroids in man. J Clin Endocrinol Metab 41:290–304
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Tanaka, H., Hirano, F., Nomura, Y. et al. Relative glucocorticoid potency revisited. Rheumatol Int 14, 9–12 (1994). https://doi.org/10.1007/BF00302665
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DOI: https://doi.org/10.1007/BF00302665