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Metabolism of inositol 1,4,5-trisphosphate in squid photoreceptors

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Summary

Inositol 1,4,5-trisphosphate (InsP3) is rapidly formed in squid photoreceptors in response to light, where it is converted sequenctially into inositol bisphosphate (InsP2) and inositol monophosphate (InsP1). All of the InsP3 appears to be degraded to inositol 1,4-bisphosphate via an InsP3-phosphatase, which is characterized in this study. The enzyme is water-soluble and present in the light-transducing distal segments of squid photoreceptors. It has a Km of 50 μM for InsP3, requires Mg++ for its activity, is maximally active at neutral pH, specifically hydrolyses the 5-phosphate and is inhibited by 2,3-diphosphoglycerate. In these respects, InsP3-phosphatase of squid is very similar to the enzymes of other cells. Since no InsP4 or more highly phosphorylated inositols are found in squid photoreceptors, the InsP3-phosphatase may be important in the regulation of InsP3 concentration within these cells.

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Abbreviations

InsP 1 , InsP 2 , InsP 3 , InsP 4 , InsP 6 :

inositol monobis-, tris-, tetrakis-, hexakisphosphate, respectively

2,3-DPG :

2,3-diphosphoglycerate

EDTA :

ethylene diamine tetraacetic acid

DTT :

dithiothreitol

Hepes :

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

PMSF :

phenylmethylsulfonyl fluoride

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Author notes

  1. Susan F. Wood

    Present address: Dept. of Neuroscience, The Johns Hopkins University School of Medicine, 725 N. Wolfe St., 21205, Baltimore, MD, USA

Authors and Affiliations

  1. The Marine Biological Laboratory, 02543, Woods Hole, MA

    Susan F. Wood, Ete Z. Szuts & Alan Fein

  2. Boston University Marine Program, 02543, Woods Hole, MA

    Susan F. Wood

  3. Department of Physiology, Boston University School of Medicine, 02118, Boston, MA, USA

    Ete Z. Szuts

  4. Department of Physiology, University of Connecticut Health Center, 06032, Farmington, CT, USA

    Alan Fein

Authors
  1. Susan F. Wood
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  2. Ete Z. Szuts
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  3. Alan Fein
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Wood, S.F., Szuts, E.Z. & Fein, A. Metabolism of inositol 1,4,5-trisphosphate in squid photoreceptors. J Comp Physiol B 160, 293–298 (1990). https://doi.org/10.1007/BF00302595

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  • DOI: https://doi.org/10.1007/BF00302595

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