Summary
Bacteria with the ability to form L-phenylalanine from acetamidocinnamic acid were isolated from several soils. Among them, strain no. S-7 and strain no. N-7 were identified as Alcaligenus faecalis S-7 and Bacillus sphaericus N-7, respectively. The L-phenylalanine-forming enzyme systems in both bacteria were found to be inducible and intracellular. With intact cells of both bacteria and 40 mg/ml as wet base, 10 mg/ml acetamidocinnamic acid was utilized, and 7.7 mg/ml L-phenylalanine in a molar yield of 94% was produced after 72h incubation. The pathway of L-phenylalanine formation is considered to take the following course: acetamidocinnamic acid is deacetylated to α-amino cinnamic acid; this is spontaneously changed to phenylpyruvic acid, and L-phenylalanine is formed by transamination.
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Nakamichi, K., Nabe, K., Yamada, S. et al. L-Phenylalanine formation from acetamidocinnamic acid by newly isolated bacteria. Appl Microbiol Biotechnol 19, 100–105 (1984). https://doi.org/10.1007/BF00302448
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DOI: https://doi.org/10.1007/BF00302448