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The yeast protein Gcr1p binds to the PGK UAS and contributes to the activation of transcription of the PGK gene

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Abstract

Analysis of the upstream activation sequence (UAS) of the yeast phosphoglycerate kinase gene (PGK) has demonstrated that a number of sequence elements are involved in its activity and two of these sequences are bound by the multifunctional factors Rap1p and Abftp. In this report we show by in vivo footprinting that the regulatory factor encoded by GCR1 binds to two elements in the 3′ half of the PGK UAS. These elements contain the sequence CTTCC, which was previously suggested to be important for the activity of the PGK UAS and has been shown to be able to bind Gcrlp in vitro. Furthermore, we find that Gcr1p positively influences PGK transcription, although it is not responsible for the carbon source dependent regulation of PGK mRNA synthesis. In order to mediate its transcriptional influence we find that Gcrtp requires the Rap1p binding site, in addition to its own, but not the Abf1p site. As neither a Rapip nor a Gcr1p binding site alone is able to activate transcription, we propose that Gcr1p and Rapip interact in an interdependent fashion to activate PGK transcription.

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Author notes

  1. Y. A. L. Henry

    Present address: LBME, CNRS, 118 Route de Narbonne, 31062, Toulouse Cedex, France

  2. C. A. Stanway

    Present address: Xenova Ltd, 240 Bath Road, SL1 4EF, Slough, UK

Authors and Affiliations

  1. Department of Biochemistry, Oxford University, South Parks Road, OX1 3QU, Oxford, UK

    Y. A. L. Henry, A. Chambers, S. M. Kingsman & C. A. Stanway

  2. Department of Immunology and Medical Microbiology, University of Florida College of Medicine, 32610-0266, Florida, Gainesville, USA

    M. C. López & H. V. Baker

  3. Department of Plant Sciences, Oxford University, South Parks Road, OXI 3RB, Oxford, UK

    J. M. Gibbs & C. A. Stanway

  4. Department of Genetics, Queens' Medical Centre, University of Nottingham, NG7 2UH, Nottingham, UK

    A. Chambers

Authors
  1. Y. A. L. Henry
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  2. M. C. López
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  3. J. M. Gibbs
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  4. A. Chambers
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  5. S. M. Kingsman
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  6. H. V. Baker
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  7. C. A. Stanway
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Communicated by C. P. Hollenberg

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Henry, Y.A.L., López, M.C., Gibbs, J.M. et al. The yeast protein Gcr1p binds to the PGK UAS and contributes to the activation of transcription of the PGK gene. Molec. Gen. Genet. 245, 506–511 (1994). https://doi.org/10.1007/BF00302263

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  • DOI: https://doi.org/10.1007/BF00302263

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