Abstract
A 29 kb shuttle cosmid vector, pTYS507, was constructed from a cryptic Micromonospora griseorubida plasmid and the Escherichia coli cosmid pJB8. Subcloning of mycinamicin II biosysnthesis genes in pTYS507 led to the identification of a DNA region that could complement a mutant of M. griseorubida that lacked both hydroxylase and epoxidase activities. Nucleotide sequence and mutational analysis suggested that a single P-450-like protein catalyzes both reactions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Benjamin PU, Gunsalus IC, Stephen GS (1986) Nucleotide sequence of the Pseudomonas putida cytochrome P-450cam gene and its expression in Escherichia coli. J Biol Chem 261:1158–1163
Bibb MJ, Findlay PR, Johnson MW (1984) The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of proteincoding sequences. Gene 30:157–166
Donadio S, Hutchinson CR (1991) Cloning and characterization of the Saccharopolyspora erythraea fdxA gene encoding ferredoxin. Gene 100:231–235
Gladek A, Zakrzewska J (1984) Genomic size of Streptomyces. FEMS Microbiol Lett 24:73–76
Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557–80
Haydock SF, Dowson JA, Dhillon N, Roberts GA, Cortes J, Leadlay PF (1991) Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases. Mol Gen Genet 230:120–128
Hobbs AA, Mattschoss LA, May BK, Williams KE, Elliott WH (1986) The cDNA and protein sequence of a phenobarbital-induced chicken cytochrome P-450. J Biol Chem 261:9444–9449
Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM, Schrempf H (1985) Genetic manipulations of Streptomyces: A laboratory manual. The John Innes Foundation, Norwich, UK
Horii M, Ishizaki T, Paik SY, Manome T, Murooka Y (1990) An operon containing the genes for cholesterol oxidase and a cytochrome P-450-like protein from a Streptomyces sp. J Bacteriol 172:3644–3653
Ish-Horowicz D, Burke JF (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9:2989–2998
Inouye M, Morohoshi T, Horinouchi S, Beppu T (1994a) Cloning and sequences of two macrolide-resistance-encoding genes from mycinamicin-producing Micromonospora griseorubida. Gene 141:39–46
Inouye M, Suzuki H, Takada Y, Muto N, Horinouchi S, Beppu T (1994b) A gene encoding mycinamicin III O-methyltransferase from Micromonospora griseorubida. Gene 141:121–124
Katz E, Thompson CJ, Hopwood DA (1983) Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. J Gen Microbiol 129:2703–2714
Kieser T (1984) Factors affecting the isolation of cccDNA from Streptomyces lividans and Escherichia coli. Plasmid 12:19–36
Kimura S, Gonzalez FJ, Nebert DW (1984) The murine Ah locus: comparison of the complete cytochrome P1-450 and P3-450 cDNA nucleotide and amino acid sequences. J Biol Chem 259:10705–10713
Lydiate DJ, Malpartida F, Hopwood DA (1985) The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Gene 35:223–235
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Morohashi K, Fujii-Kuriyama Y, Okada Y, Sogawa K, Hirose T, Inayama S, Omura T (1984) Molecular cloning and nucleotide sequence of cDNA for mRNA of mitochondrial cytochrome P-450 (SCC) of bovine adrenal cortex. Proc Natl Acad Sci USA 81:4647–4651
Nebert DW, Gonzalez FJ (1987) P450 genes: structure, evolution, and regulation. Annu Rev Biochem 56:945–993
Omer CA, Lenstra R, Litle PJ, Dean C, Tepperman JM, Leto KJ, Romesser JA, O'Keefe DP (1990) Genes for two herbicide-inducible cytochromes P-450 from Streptomyces griseolus. J Bacteriol 172:3335–3345
Poulos TL, Finzel BC, Howard AJ (1987) High resolution crystal structure of cytochrome P450CAM. J Mol Biol 195:687–700
Saito I, Stark GR (1986) Charomids; cosmid vectors for efficient cloning and mapping of large or small restriction fragments. Proc Natl Acad Sci USA 83:8664–8668
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Satoi S, Muto N, Hayashi M, Fujii T, Otani M (1980) Mycinamicins, new macrolide antibiotics. I. Taxonomy, production, isolation, characterization and properties. J Antibiot 33:364–376
Shafiee A, Hutchinson CR (1988) Purification and reconstruction of the electron transport components for 6-deoxyerythronolide B hydroxylase, a cytochrome P-450 enzyme of macrolide antibiotic (erythromycin) biosynthesis. J Bacteriol 170:1548–1553
Suzuki H, Takenaka S, Kinoshita K, Morohoshi T (1990) Biosynthesis of mycinamicins by a blocked mutant of Micromonospora griseorubida. J Antibiot 43:1508–1511
Vieira J, Messing J (1987) Production of single stranded plasmid DNA. Methods Enzymol 153:3–11
Weber JM, Leung JO, Swanson SJ, Idler KB, McAlpine JB (1991) An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea. Science 252:114–117
White PC, New MI, Dupont B (1986) Structure of human steroid 21-hydroxylase genes. Proc Natl Acad Sci USA 80:5111–5115
Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
Author information
Authors and Affiliations
Additional information
Communicated by J. Lengeler
Rights and permissions
About this article
Cite this article
Inouye, M., Takada, Y., Muto, N. et al. Characterization and expression of a P-450-like mycinamicin biosynthesis gene using a novel Micromonospora-Escherichia coli shuttle cosmid vector. Molec. Gen. Genet. 245, 456–464 (1994). https://doi.org/10.1007/BF00302258
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00302258