Skip to main content

Advertisement

SpringerLink
Log in

Bone mineral density in the femur and lumbar vertebrae decreases after twelve weeks of diabetes in spontaneously diabetic-prone BB/Worcester rats

  • Laboratory Investigations
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Abstract

The accumulated data indicate that bone mineral density (BMD) is decreased in humans with insulin-dependent diabetes mellitus. The purpose of this study was to prospectively determine sequential lumbar and femoral BMD utilizing dual energy X-ray absorptiometry in rats that spontaneously become diabetic to determine if weight and blood glucose control would prevent the diabetes-related bone mass changes. BMD of the lumbar spine and femur was measured prior to the onset of diabetes and at 3-week intervals after the diagnosis of diabetes for 12 weeks in 14 diabetes-prone BB/Wor rats (DP) and eight diabetes-resistant BB/Wor control rats (DR). At 12 weeks, the lumbar (0.238±0.013 vs 0.262±0.007 g/cm2, P<0.001) and femoral (0.313±0.013 vs 0.343±0.013 g/cm2, P<0.001) BMD were significantly lower in the DP rats despite significantly greater body weights (387±26 vs 329±46 g, P<0.001) and plasma glucose levels of only 178 mg/dl. There was no difference in plasma values of calcium, phosphorus, osteocalcin, or tartrate-resistant acid phosphatase between groups or differences in osteoblast numbers in histologic sections. There was a significant (P<0.001) decrease in plasma creatinine in the diabetic animals. The results indicate that in this animal model of type I diabetes, spine and femoral BMD do not increase comparable to control despite weight and blood glucose control. This would suggest that the diabetic condition itself affects bone mass in the absence of weight loss and poor blood glucose control.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Levin ME, Boisseau VC, Avioli LV (1976) Effects of diabetes mellitus on bone mass in juvenile and adult-onset diabetes. N Engl J Med 294:241–245

    Google Scholar 

  2. McNair P (1988) Bone mineral metabolism in human type 1 (insulin dependent) diabetes mellitus. Dan Med Bull 35:109–121

    Google Scholar 

  3. McNair P, Madsbad S, Christensen MS, Christiansen C, Faber OK, Binder C (1979) Transbolpathogenesis. Acta Endocrinol (Copenh) 90:463–472

    Google Scholar 

  4. Saggese G, Bertelloni S, Baroncelli GI, Federico G, Calisti L, Fusaro C (1989) Bone demineralization and impaired mineral metabolism in insulin-dependent diabetes mellitus: a possible role of magnesium deficiency. Helv Paediatr Acta 43:405–414

    Google Scholar 

  5. Santiago JV, McAlister WH, Ratzan SK, Bussman Y, Haymond MW, Shackelford G, Weldon VV (1977) Decreased cortical thickness and osteopenia in children with diabetes mellitus. J Clin Endocrinol Metab 45:845–848

    Google Scholar 

  6. Shore RM, Chesney RW, Mazess RB, Rose PG, Bargman GJ (1981) Osteopenia in juvenile diabetes. Calcif Tissue Int 33:455–457

    Google Scholar 

  7. Soejima K, Landing BH (1986) Osteoporosis in juvenile-onset diabetes mellitus: morphometric and comparative studies. Pediatr Pathol 6:289–299

    Google Scholar 

  8. Wakasugi M, Wakao R, Tawata M, Gan N, Koizumi K, Onaya T (1993) Bone mineral density measured by dual energy x-ray absorptiometry in patients with non-insulin-dependent diabetes mellitus. Bone 14:29–33

    Google Scholar 

  9. Weinstock RS, Goland RS, Shane E, Clemens TL, Lindsay R, Bilezikian JP (1989) Bone mineral density in women with Type II diabetes mellitus. J Bone Miner Res 4:97–101

    Google Scholar 

  10. Verhaeghe J, Van Herck E, Visser WJ, Suiker AMH, Thomasset M, Einhorn TA, Faierman E, Bouillon R (1990) Bone and mineral metabolism in BB rats with long-term diabetes: decreased bone turnover and osteoporosis. Diabetes 39:477–482

    Google Scholar 

  11. Crisa L, Mordes JP, Rossini AA (1992) Autoimmune diabetes mellitus in the BB rat. Diabetes Metab Rev 8:9–37

    Google Scholar 

  12. Rossini AA, Mordes JP, Like AA (1985) Immunology of insulin-dependent diabetes mellitus. Annu Rev Immunol 3:289–320

    Google Scholar 

  13. Ongphiphadhanakul B, Alex S, Braverman LE, Baran DT (1992) Excessive 1-thyroxine therapy decreases femoral bone mineral densities in the male rat: effect of hypogonadism and calcitonin. J Bone Miner Res 7:1227–1231

    Google Scholar 

  14. Dixit PK, Ekstrom RA (1980) Decreased breaking strength of diabetic rat bone and its improvement by insulin treatment. Calcif Tissue Int 32:195–199

    Google Scholar 

  15. Einhorn TA, Boskey AL, Gundberg CM, Vigorita VJ, Devlin VJ, Beyer MM (1988) The mineral and mechanical properties of bone in chronic experimental diabetes. J Orthop Res 6:317–323

    Google Scholar 

  16. Hernberg CA (1952) The bone structure in alloxan-induced diabetes mellitus in rats. Acta Med Scand 142:274–283

    Google Scholar 

  17. Hough S, Avioli LV, Bergfeld MA, Fallon MD, Slatopolsky E, Teitelbaum SL (1981) Correction of abnormal bone and mineral metabolism in chronic streptozotocin-induced diabetes mellitus in the rat by insulin therapy. Endocrinology 108:2228–2234

    Google Scholar 

  18. Ishida H, Seino Y, Taminato T, Usami M, Takeshita N, Seino Y, Tsutsumi C, Moriuchi S, Akiyama Y, Hara K, Imura H (1988) Circulating levels and bone contents of bone-carboxyglutamic acid-containing protein are decreased in streptozotocin-induced diabetes. Diabetes 37:702–706

    Google Scholar 

  19. Umpierrez GE, Goldstein S, Phillips LS, Spanheimer RG (1989) Nutritional and hormonal regulation of articular collagen production in diabetic animals. Diabetes 38:758–763

    Google Scholar 

  20. Spanheimer R (1991) Inhibition of collagen production by diabetic rat serum: response to insulin and insulin-like growth factor-I added in vitro. Endocrinology 129:3018–3026

    Google Scholar 

  21. Levy J, Reid I, Halstad L, Gavin JR III, Avioli V (1989) Abnormal cell calcium concentrations in cultured bone cells obtained from femurs of obese and noninsulin-dependent diabetic rats. Calcif Tissue Int 44:131–137

    Google Scholar 

  22. Raskin P, Stevenson MRM, Barilla DE, Pak CYC (1978) The hypercalciuria of diabetes mellitus: its amelioration with insulin. Clin Endocrinol (Oxf) 9:329–335

    Google Scholar 

  23. Schneider LE, Schedl HP, McCain T, Haussler MR (1977) Experimental diabetes reduces circulating 1,25-dihydroxyvitamin D in the rat. Science 196:1452–1454

    Google Scholar 

  24. Schneider LE, Schedl HP (1972) Diabetes and intestinal calcium absorption in the rat. Am J Physiol 223:1319–1323

    Google Scholar 

  25. Gertner J, Horst R, Tamborlane W (1979) Mineral metabolism and vitamin D status in juvenile diabetics (JOD): changes following normalization of plasma glucose (PG) with a protable infusion pump (abstract). Diabetes 28:354

    Google Scholar 

  26. DeNicola AF, Fridman O, DeCastillo EJ, Foglia VG (1977) Abnormal regulation of adrenal function in rats with streptozotocin diabetes. Horm Metab Res 9:469–473

    Google Scholar 

  27. Stancakova A, Merstenova E, Vajo J, Valkova M (1978) The excretion of free cortisol, cortisone, cortisol sulfate and cortisone sulfate in peripheral vascular disease, diabetes mellitus and hyperthyroidism. Horm Metab Res 10:539–544

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Endocrinology, University of Massachusetts Medical Center, 01655, Worcester, Massachusetts, USA

    C. E. Waud & D. T. Baran

  2. Department of Cell Biology, University of Massachusetts Medical Center, 01655, Worcester, Massachusetts, USA

    S. C. Marks Jr.

  3. Department of Pharmacology, University of Massachusetts Medical Center, 01655, Worcester, Massachusetts, USA

    R. Lew

  4. Division of Orthopedics, University of Massachusetts Medical Center, 01655, Worcester, Massachusetts, USA

    C. E. Waud, S. C. Marks Jr., R. Lew & D. T. Baran

Authors
  1. C. E. Waud
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. C. Marks Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Lew
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. T. Baran
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Waud, C.E., Marks, S.C., Lew, R. et al. Bone mineral density in the femur and lumbar vertebrae decreases after twelve weeks of diabetes in spontaneously diabetic-prone BB/Worcester rats. Calcif Tissue Int 54, 237–240 (1994). https://doi.org/10.1007/BF00301685

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00301685

Key words

Search

Navigation