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In vivo nuclear magnetic resonance studies on the lugworm Arenicola marina. II Seasonal changes of metabolism

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Abstract

In vivo 31P- and 13C-NMR spectra of the lugworm Arenicola marina (Polychaeta, Annelida) gathered between 1988 and 1994 at different times of the year were evaluated for seasonally dependent metabolic changes. Beside the typical 31P-NMR signals of ATP and (phospho)taurocyamine, other seasonally dependent signals were observed: from January to March an intensive signal at 1.4–1.8 ppm was identified as inorganic phosphate compartmented in an acidic intestinal lumen. Between April and September signals at 1.2–1.4 ppm were assigned to phosphodiester. Starting in July males showed a second phosphagen signal [(phospho)creatine of spermatozoa, cf. Kamp and Juretschke (1989a)] whose intensity increased until spawning in September. The (phospho)taurocyamine/ATP ratio was also dependent on the season. In January or February the ratio reached 11, while in summer and autumn the ratio was between 4 and 5. As verified by biochemical assays this effect was caused by a significant decrease of ATP in the lugworm body wall during winter (December–February). The phosphagen (phospho)taurocyamine and the respective unphosphorylated guanidine taurocyamine remained constant throughout the year. Levels of free inorganic phosphate incurred similar changes to ATP. 13C-NMR spectra of lugworms showed a dramatic change in lipid stores. They were below the detection limit between January and March but developed into the most intensive signals during summer. The most abundant amino acids, glycine and alanine, were observed throughout the year while glycogen could not be detected in the 13C-NMR spectra. After treating tissue extracts with amyloglucosidase, the signals of the hydrolytic product glucose were recorded indicative of NMR-invisible glycogen stores.

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Abbreviations

AN :

adenine nucleotides

NMR :

nuclear magnetic resonance

P i :

inorganic phosphate

(P)Cr :

(phospho)creatine

PDE :

phosphodiester

PME :

phosphomonoester

PPA :

phenylphosphonic acid

(P)Tc :

(phospho)taurocyamine

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Authors and Affiliations

  1. Institut für Biophysikalische Chemie, Johann Wolfgang Goethe-Universität, Marie-Curie-Str. 9, D-60439, Frankfurt/M, Germany

    H. P. Juretschke

  2. Institut für Zoophysiologie der Westfälischen Wilhelms-Universität, Hindenburgplatz 55, D-48143, Münster, Germany

    G. Kamp

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  1. H. P. Juretschke
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  2. G. Kamp
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Communicated by H. Langer

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Juretschke, H.P., Kamp, G. In vivo nuclear magnetic resonance studies on the lugworm Arenicola marina. II Seasonal changes of metabolism. J Comp Physiol B 165, 153–160 (1995). https://doi.org/10.1007/BF00301479

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