Rheumatology International

, Volume 13, Issue 4, pp 147–150 | Cite as

The clinical significance of the detection of anti-Ro/SS-A and anti-La/SS-B autoantibodies using purified recombinant proteins in primary Sjögren's syndrome

  • H. M. Markusse
  • C. H. A. Veldhoven
  • A. J. G. Swaak
  • R. T. Smeenk


Recently, an enzyme-linked immunosorbent assay (ELISA), using purified recombinant non-fusion proteins, has been introduced to detect and quantify Ro/SS-A and La/SS-B autoantibodies. We compared this method with anti-Ro/SS-A and anti-La/SS-B detection by means of counterimmunoelectrophoresis and immunoblotting in patients with primary Sjögren's syndrome (SS), patients suspected of the syndrome and controls. The sensitivity and specificity of the newly developed ELISA for anti-Ro/SS-A were 53% and 100%, respectively, and for anti-La/SS-B, 40% and 98%, respectively. No significant difference was found between these results and those obtained from both other assays. Titres of Ro/SS-A and La/SS-B autoantibodies correlated with the presence of an abnormal parotid gland sialogram and hypergammaglobulinaemia. We concluded that the new ELISA did not enhance the diagnostic yield in cases of suspicion of primary Sjögren's syndrome. Longitudinal studies of large groups of patients with primary Sjögren's syndrome are necessary to demonstrate whether following the course of the titres of these autoantibodies would be of value for prediction of disease exacerbations.

Key words

Anti-Ro/SS-A Anti-La/SS-B Recombinant antigen Autoantibody Sjögren's syndrome 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Moutsopoulos HM, Chused TM, Mann DL et al (1980) Sjögren's syndrome (sicca syndrome): current issues. Ann Intern Med 92:212–226Google Scholar
  2. 2.
    Manthorpe R, Oxholm P, Prause JU, Schiodt M (1986) The Copenhagen criteria for Sjögren's syndrome. Scand J Rheumatol [Suppl] 61:19–21Google Scholar
  3. 3.
    Manthorpe R, Andersen V, Jensen OA, Oxholm P, Prause JU, Schiodt M (1986) Editorial comments to the four sets of criteria for Sjögren's syndrome. Scan J Rheumatol [Suppl] 61:31–35Google Scholar
  4. 4.
    Meilof JF, Smeenk RJT (1992) Autoantibodies and their target antigens in Sjögren's syndrome. Neth J Med 240:140–147Google Scholar
  5. 5.
    Veldhoven CHA, Meilof JF, Huisman JG, Smeenk RJT (1992) The development of a quantitative assay for the dection of anti-Ro/SS-A and anti-La/SS-B autoantibodies using purified recombinant proteins. J Immunol Methods 151:177–189Google Scholar
  6. 6.
    Kurata N, Tan EM (1976) Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis. Arthritis Rheum 19:574–580Google Scholar
  7. 7.
    Chan EKL, Francœur A, Tan EM (1986) Epitopes, structural domains and asymmetry of amino acid residues in SS-B/La nuclear protein. J Immunol 136:3744–3749Google Scholar
  8. 8.
    Towbin II, Stähelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354Google Scholar
  9. 9.
    Dunn SD (1986) Effects of the modification of transfer buffer composition and the renaturation of proteins in gel on the recognition of proteins on Western blots by monoclonal antibodies. Anal Biochem 157:144–149Google Scholar
  10. 10.
    Westgeest AAA, Van den Brink HG, De Jong J, Swaak AJG, Smeenk RJT (1987) Antinuclear antibodies in patients with systemic lupus erythematosus: a comparison of counterimmunoelectrophoresis and immunoblotting. Rheumatol Int 7:77–82Google Scholar
  11. 11.
    Chisholm DM, Mason DK (1968) Labial salivary gland biopsy in Sjögren's disease. J Clin Pathol 21:656–660Google Scholar
  12. 12.
    Markusse HM, Van Putten WIJ, Breedveld FC, Oudkerk M (1993) Digital subtraction sialography of the parotid glands in primary Sjögren's syndrome. J Rheumatol 20:279–283Google Scholar
  13. 13.
    Miyachi K, Naito M, Maeno Y, et al (1983) Sialographic study in patients with and without a antibodies to Sjögren's syndrome A (Ro). J Rheumatol 10:387–394Google Scholar
  14. 14.
    Alexander EL, Arnett FC, Provost TT, Stevens MB (1983) Sjögren's syndrome: association of Anti-Ro (SS-A) antibodies with vasculitis, hematologic abnormalities, and serologic hyperreactivity. Ann Intern Med 98:155–159Google Scholar
  15. 15.
    Molina R, Provost TT, Alexander EL (1985) Two types of inflammatory vascular disease in Sjögren's syndrome. Arthritis Rheum 28:1251–1258Google Scholar
  16. 16.
    Harley JB, Alexander EL, Bias WM, et al (1986) Anti-Ro (SS-A) and anti-La (SS-B) in patients with Sjögren's syndrom. Arthritis Rheum 29:196–206Google Scholar
  17. 17.
    Swaak AJG, Aarden LA, Statius van Eps LW, Feltkamp TEW (1979) Anti-dsDNA and complement profiles as prognostic guides in systemic lupus erythematosus. Arthritis Rheum 22:226–235Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • H. M. Markusse
    • 1
  • C. H. A. Veldhoven
    • 2
    • 3
  • A. J. G. Swaak
    • 1
  • R. T. Smeenk
    • 2
    • 3
  1. 1.Department of RheumatologyDr. Daniel den Hoed ClinicRotterdamThe Netherlands
  2. 2.Department of Autoimmune DiseasesRed Cross Blood Transfusion ServiceAmsterdamThe Netherlands
  3. 3.Department of Developmental Research, Central Laboratory of the NetherlandsRed Cross Blood Transfusion ServiceAmsterdamThe Netherlands

Personalised recommendations