Summary
In the pathogenesis of late Lyme borreliosis the relative importance of the causative organism,Borrelia burgdorferi, and the immune response of the host, including autoimmune phenomena, is not yet known. We describe a 7-year-old boy with Lyme arthritis from whom two synovial fluid samples were obtained 5 months apart and up to 17 months after the first appearance of arthritis. Both synovial fluid specimens were shown to contain borrelial DNA by nested polymerase chain reaction for the amplification of portions of the genes for flagellin and OspA. Thus,Borrelia burgdorferi may persist within the joint even during late stages of the disease.
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Eichenfield AH, Goldsmith DP, Benach JL, Ross AH, Loeb FX, Doughty RA, Athreya BH (1986) Childhood Lyme arthritis: experience in an endemic area. J Pediatr 109:753–758
Goebel KM, Krause A, Neurata F (1988) Acquired transient autoimmune reactions in Lyme arthritis: correlation between rheumatoid factor and disease activity. Scand J Rheumatol [Suppl 75]:314–317
Martin R, Ortlauf J, Sticht-Groh V, Bogdahn U, Goldmann SF, Mertens HG (1988)Borrelia burgdorferi-specific and autoreactive T-cell lines from cerebrospinal fluid in Lyme radiculomyelitis. Ann Neurol 24:509–516
Steere AC, Dwyer E, Winchester R (1990) Association of chronic Lyme arthritis with HLA DR4 and HLA DR2 alleles. N Engl J Med 323:219–223
Schmidli J, Hunziler T, Moesli P, Schaad UB (1988) Cultivation ofBorrelia burgdorferi from joint fluid three months after treatment of facial palsy due to Lyme borreliosis. J Infect Dis 158:905–906
Persing DH, Rys PN, Blaricom G van, Dodge DE, White TJ, Schoen RT, Rahn DW, Malawista SE (1990) Multi-target detection ofBorrelia burgdorferi-associated DNA sequences in synovial fluid of patients with arthritis (abstract). Arthritis Rheum 33 [Suppl 9]:S 36
Huppertz HI, Karch H (1991) Die Lyme-Arthritis im Kindesalter: Monarthritis des Kniegelenks, klinisch nicht unterscheidbar von Monarthritiden unbekannter Ursache. Monatsschr Kinderheilkd 139:759–764
Gassmann GS, Jacobs E, Deutzmann R, Göbel UB (1991) Analysis of theBorrelia burgdorferi GeHo fla gene and antigen characterisation of its gene product. J Bacteriol 173:1452–1459
Bergström S, Bundoc VG, Barbour AG (1989) Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaeteBorrelia burgdorferi. Mol Microbiol 3:479–486
Huppertz HI, Sticht-Groh V, Schwan T (1986) Borderline antibody response in initial stages of lymphocytic meningitis does not rule out borreliosis. Lancet II:1468–1469
Hammer M, Zeidler H, Klimsa S, Heesemann J (1990)Yersinia enterocolitica in the synovial membrane of patients with Yersinia-induced arthritis. Arthritis Rheum 33:1795–1800
Viitanen AM, Arstila TP, Lahesmaa R, Granfors K, Skurnik M, Toivanen P (1991) Application of the polymerase chain reaction and immunofluorescence techniques to the detection of bacteria in Yersinia-triggered reactive arthrritis. Arthritis Rheum 34:89–96
Krause A, Brade V, Schoerner C, Solbach W, Kalden JR, Burmester GR (1991) T cell proliferation induced byBorrelia burgdorferi in patients with Lyme borreliosis. Arthritis Rheum 34:393–402
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Karch, H., Huppertz, H.I. Repeated detection ofBorrelia burgdorferi DNA in synovial fluid of a child with Lyme arthritis. Rheumatol Int 12, 227–229 (1992). https://doi.org/10.1007/BF00301006
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DOI: https://doi.org/10.1007/BF00301006