The ultrastructure of club cells and neighbouring filament cells and leucocytes in the epidermis of carp, was studied under normal conditions and after exposure to several stressors: acid water, heavy metals, organic manure, brackish water and wounding. The effects of the stressors were remarkably similar. The club cells increased in size and contained more endoplasmic reticulum and Golgi areas. In both control and stressed fish, most mitotic figures of the filament cells were found adjacent to club cells, as was demonstrated after colchicine injection. Whereas in the controls apoptosis of filament cells was scarce and limited to the upper layer of the epithelium, in the stressed fish it was commonly seen in close proximity to the club cells but not in other mid-epidermal parts of the epithelium. This indicates that club cells influence the cellular kinetics of the filament cells. Under stress conditions leucocytes infiltrated the epidermis. Some were seen inside club cells. Apparently these leucocytes were taken up in phagosomes and subsequently they showed signs of necrotic degeneration. Leucocyte incorporation and degeneration in club cells were not observed in control fish. Control of the cellular turnover of filament cells and the elimination of leucocytes may represent new functions for club cells, which have mainly been associated with the production of pheromones.
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Al-Hassan JM, Thomson M, Ali M, Criddle RS (1987) Toxic and pharmacologically active secretions from the Arabian Gulf catfish (Arius thalassinus, Ruppel). Toxicol Toxin Rev 6:1–43
Bowen ID (1981) Techniques for demonstrating cell death. In: Bowen ID, Lockshin RA (eds) Cell death in Biology and Pathology. Chapman and Hall, London New York, pp 379–466
Budtz PE, Spies I (1989) Epidermal tissue homeostasis: Apoptosis and cell emigration as mechanisms of controlled cell deletion in the epidermis of the toad, Bufo bufo. Cell Tissue Res 256:475–486
Fujita T, Kanno T, Kobayashi S (1988) The Paraneuron. Springer, Berlin Heidelberg New York, pp 118–131
Gould VE, Moll R, Moll I, Lee I, Franke WW (1985) Biology of disease. Neuroendocrine (Merkel) cells of skin: hyperplasias, dysplasias, and neoplasms. Lab Invest 52:334–353
Henkart PA (1985) Mechanism of lymphocyte mediated cytotoxicity. Ann Rev Immunol 3:31–58
Iger Y (1992) Adaptive reactions in the skin of the common carp (Cyprinus carpio) under the impact of ecological factors and wounding. PhD Thesis, The Hebrew University of Jerusalem, Jerusalem, Israel
Iger Y, Abraham M (1990) The process of skin healing in experimentally wounded carp. J Fish Biol 36:421–437
Iger Y, Wendelaar Bonga SE (1994) Cellular responses of the skin of carp (Cyprinus carpio) exposed to acidified water. Cell Tiss Res 275:481–492
Iger Y, Abraham M, Dotan A, Fattal B, Rahamim E (1988) Cellular responses in the skin of carp maintained in organically fertilized water. J Fish Biol 33:711–720
Lufty RG (1964) Studies on the epidermis of the catfish Synodontis schall Bl. Sch. Ain Shams Sci Bull Cairo 10:153–163
Parsons DF, Marko M, Braun SJ, Wansor KJ (1983) “Dark cells” in normal, hyperplastic and promotor-treated mouse epidermis studied by conventional and high-voltage electron microscopy. J Invest Dermatol 81:62–67
Pfeiffer W (1977) The distribution of fright reaction and alarm substance cells in fishes. Copeia 1977:653–665
Ralphs JR, Benjamin M (1992) Chondroitin and keratan sulphate in the epidermal club cells of teleosts. J Fish Biol 39:473–475
Smith RJF (1982) The adaptive significance of the alarm substance. Fright reaction system. In: Hara TJ (ed) Chemoreception in fishes. Elsevier North Holland, Amsterdam, pp 327–342
Suzuki K (1986) Morphological and phagocytic characteristics of peritoneal exudate cells in tilapia, Oreochromis niloticus (Trewawas), and carp, Cyprinus carpio L. J Fish Biol 29:349–364
Suzuki Y, Kaneko T (1986) Demonstration of the mucous hemagglutinin in the club cells of eel skin. Dev Comp Immunol 10:509–518
Wendelaar Bonga SE, Van der Meij CJM (1989) Degeneration and death, by apoptosis and necrosis, of the pavement and chloride cells in the gills of the teleost Oreochromis mossambicus. Cell Tissue Res 255:235–243
Whitear M (1986) The skin of fishes including cyclostomes. In: Bereiter-Hahn J, Matoltsy AG, Richards KS (eds) Biology of the integument vol 2: Vertebrates. Springer, Berlin Heidelberg New York, pp 8–38
Whitear M, Mittal AS (1983) Fine structure of the club cells in the skin of ostariophysan fish. Z Mikrosk Anat Forsch 97:141–157
Whitear M, Zaccone G (1984) Fine structure and histochemistry of club cells in the skin of three species of eel. Z Mikrosk Anat Forsch 98:481–501
Whitear M, Zaccone G, Fasulo S, Licata A (1991a) Fine structure of the axillary gland in the brown bullhead (Ictalurus nebulosus). J Zool Lond 224:669–676
Whitear M, Zaccone G, Ricca MB and Lo Cascio P (1991b) Fine structure and histochemistry of the venom gland in the Indian stinging catfish (Heteropneustes fossilis). J Zool Lond 224:479–489
Wyllie AH (1981) Cell death: a new classification separating apoptosis from necrosis. In: Bowen ID, Lockshin RA (eds) Cell death in biology and pathology. Chapman and Hall, London New York, pp 9–34
Zaccone G, Tagliafierro G, Fasulo S, Contini A, Ainis L, Ricca MB (1990) Serotonin-like immunoreactivity in the epidermal club cells of teleost fishes. Histochemistry 93:355–357
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Iger, Y., Abraham, M. & Wendelaar Bonga, S.E. Response of club cells in the skin of the carp Cyprinus carpio to exogenous stressors. Cell Tissue Res 277, 485–491 (1994). https://doi.org/10.1007/BF00300221
- Club cells
- Filament cells
- Cyprinus carpio (Teleostei)