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Comparison of an endogenous protein kinase C substrate in rat aorta with rat brain MARCKS

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Abstract

We have compared the properties of a rat aorta-derived protein kinase C substrate (p75) with those of 80 kDa kinase C substrates from rat brain (MARCKS) and rabbit aorta (p80). Rat aortic p75 appeared to be closely related to rat brain MARCKS on the basis of: solubility in perchloric acid and trichloroacetic acid, heat stability, isoelectric point (pI ∼ 4.2), overall V8 protease phosphopeptide map, and immunocrossreactivity with an antibody directed against the N-terminal domain of MARCKS. However, p75 could be distinguished from rat brain MARCKS and from the rabbit aorta-derived p80 on the basis of its consistently more rapid electrophoretic mobility in SDS-containing gels, and in terms of a unique proteolytic phosphopeptide found in MARCKS but not in aortic p75. We conclude that p75 probably belongs to the family of protein kinase C substrates represented by MARCKS, and that differences in post-translational processing (glycosylation) or mRNA processing may account for the unique properties of the p75 protein in rat aortic tissue.

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Abbreviations

p75:

75,000 Da protein

MARCKS:

Myristoylated Alanine-Rich C Kinase Substrate

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Authors and Affiliations

  1. MRC Signal Transduction Group, Department of Pharmacology and Therapeutics, Faculty of Medicine, The University of Calgary, T2N 4N1, Calgary, Alberta, Canada

    Dayuan Zhao & David L. Severson

  2. Endocrine Research Group, Department of Pharmacology and Therapeutics, Faculty of Medicine, The University of Calgary, T2N 4N1, Calgary, Alberta, Canada

    Dayuan Zhao & Morley D. Hollenberg

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  1. Dayuan Zhao
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  2. Morley D. Hollenberg
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  3. David L. Severson
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Zhao, D., Hollenberg, M.D. & Severson, D.L. Comparison of an endogenous protein kinase C substrate in rat aorta with rat brain MARCKS. Mol Cell Biochem 116, 163–169 (1992). https://doi.org/10.1007/BF00299395

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  • DOI: https://doi.org/10.1007/BF00299395

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