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Lipogenesis from n-butyrate in colonocytes

Action of reducing agent and S-aminosalicylic acid with relevance to ulcerative colitis

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Abstract

Cell membranes of colonic epithelial cells (CEC) in ulcerative colitis show structural abnormalities which are specific to the disease and which suggest impaired lipogenesis in CECs. Lipogenesis from [1-14C]-n-butyrate, the chief oxidative fuel of colonic epithelial cells, was measured in isolated CECs under control conditions, with or without glucose and in the presence of mercaptoacetate, a major reducing agent in the colonic lumen- Glucose significantly (p < 0.01) stimulated lipogenesis from [1-14C]-butyrate which was reversed by 5 mM mercaptoacetate. Mercaptoacetate significantly diminished CEC thiolase activity (EC 2.3.1.9). 5-Aminosalicylic acid reversed the adverse effects of mercaptoacetate in the saponifiable fraction of extracted lipids. Changes in lipogenesis due to colonic luminal reducing agents would affect the barrier function of CECs a feature relevant to the disease process of ulcerative colitis.

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References

  1. Brasitus TA, Dudeja PK: Small and large intestinal plasma membranes: structure and function. In: RC Aloia, CC Curtain and LM Gordon(eds). Lipid domains and the relationship to membrane function. New York: Alan R. Liss Inc., 1988, pp 227–254

    Google Scholar 

  2. Awad AB, Ferger L, Fink CS: Effect of dietary fat on the lipid composition and utilization of short-chain fatty acids by rat colonocytes. Lipids 25: 316–320, 1990

    Google Scholar 

  3. Listrom MB, Fenoglio-Preiser CM: The colon: normal ultrastructure and pathological patterns. In: PM Motta, H Fujita and Correr S (eds). Ultrastructure of the digestive tract. Boston: Martinus Nijhoff, 1988, pp 119–144

    Google Scholar 

  4. Sandle GI, McGlone F, Crowe P, Marsh MN: Sodium transport properties of colonic epithelium in patients with inflammatory bowel disease. (Abstr). Gastroenterology 92: 146, 1987

    Google Scholar 

  5. Gibson PR, Van de Pol E, Barratt J, Doe WF: Ulcerative colitis — disease characterised by the abnormal colonic epithelial cell? Gut 29: 516–521, 1988

    Google Scholar 

  6. Roediger WEW, Nance S: Selective reduction of fatty acid oxidation in colonocytes: correlation with ulcerative colitis. Lipids 25: 646–652, 1990

    Google Scholar 

  7. Duncan A, Kapaniris O, Roediger WEW: Measurement of mercaptoacetate levels in anaerobic batch culture of colonic bacteria. FEMS Microbiol Ecol 74: 303–308, 1990

    Google Scholar 

  8. Roediger WEW: Utilization of nutrients by isolated epithelial cells of the rat colon. Gastroenterology 83: 424–429, 1982

    Google Scholar 

  9. Roediger WEW, Truelove SC: Method of preparing isolated colonic epithelial cells (colonocytes) for metabolic studies. Gut 20: 484–488, 1979

    Google Scholar 

  10. Stansbie D, Brownsey RW, Crettaz M, Denton RM: Acute effects ‘in vivo’ of anti insulin serum on rates of fatty acid synthesis and activities of acetyl-coenzyme A carboxylase and pyruvate dehydrogenase in liver and epididymal adipose tissue of fed rats. Biochem J 160: 413–416, 1976

    Google Scholar 

  11. Gibbons GF, Pullinger CR, Munday MR, Williamson DH: Regulation of cholesterol synthesis in the liver and mammary gland of the lactating rat. Biochem J 212: 843–848, 1983

    Google Scholar 

  12. Middleton B: The oxoacyl-coenzyme A thiolase of animal tissues. Biochem J 132: 717–730, 1973

    Google Scholar 

  13. Bergmeyer HU: Methods of enzymatic analysis, 3rd ed. Vol. 4 Verlag Chemie, Weinheim, 1983, pp 353–358

    Google Scholar 

  14. Homayoun P, Bourre JM: Ketone body utilization for energy production and lipid synthesis in isolated rat brain capillaries. Biochim Biophys Acta 922: 345–350, 1987

    Google Scholar 

  15. Christensen E, Hagve T-A, Gronn M, Christophersen BO: β-oxidation of medium chain (C8–C14) fatty acids studied in isolated liver cells. Biochim Biophys Acta 1004: 187–195, 1989

    Google Scholar 

  16. Stange EF, Dietschy LM. Absolute rates of cholesterol synthesis in rat intestine in vitro and in vivo: a comparison of different substrates in slices and isolated cells. J Lipid Res 24: 72–82, 1983

    Google Scholar 

  17. Scaife JR, Tichivangana JZ: Short chain acyl-CoA synthetases in bovine rumen epithelium. Biochim Biophys Acta 619: 445–450, 1980

    Google Scholar 

  18. Awad AB, Horvath PJ, Andersen MS: Influence of butyrate on lipid metabolism, survival and differentiation of colon cancer cells. Nutr Cancer 16: 125–133, 1991

    Google Scholar 

  19. Dietschy JM, Gamel WG: Cholesterol synthesis in the intestine of man: regional differences and control mechanisms. J Clin Invest 50: 872–880, 1971

    Google Scholar 

  20. Williamson DH, Ilic V, Hughes J: Effects of short term insulin deficiency on lipogenesis and cholesterol synthesis in rat small intestine and liver in vivo. Biochem J 231: 221–223, 1985

    Google Scholar 

  21. Goodridge AG: Fatty acid synthesis in eucaryotes. In: DE Vance, JE Vance (eds). Biochemistry of lipids and membranes. Menlo Park, California: Benjamin/Cummings Publishing Corp. Inc. 1985, pp 152–155

    Google Scholar 

  22. Endemann G, Goetz PG, Edmond J, Brunengraber H: Lipogenesis from ketone bodies in the isolated perfused rat liver. J Biol Chem 257: 3434–3440, 1982

    Google Scholar 

  23. Geelen MJH, Lopes-Cardozo M, Edmond J: Acetoacetate: a major substrate for the synthesis of cholesterol and fatty acids by isolated rat hepatocytes. FEBS Letter 163: 269–273, 1983

    Google Scholar 

  24. Buckley BM, Williamson DH: Acetoacetyl-CoA synthetase: a lipogenic enzyme in rat tissues. FEBS Letters 60: 7–10, 1974

    Google Scholar 

  25. Bergstrom JD, Robbins KA, Edmond J: Acetoacetyl-CoA synthetase activity in rat liver cytosol. A regulated enzyme in lipogenesis. Biochem Biophys Res Commun 106: 856–862, 1982

    Google Scholar 

  26. Caamano GJ, Iglesias J, Marco C, Linares A: In vivo utilization of [3-14C] acetoacetate for lipid and amino acid synthesis in the 15-day-old chick. Comp Biochem Physiol 91B: 1–5, 1988

    Google Scholar 

  27. Caamano GJ, Sanchez-del-Castillo MA, Iglesias J, Garcia-Peregrin E, Linares A: Ketone body utilization in duodenum. Differential effect of fasting on lipogenesis from acetoacetate and 3-hydroxybutyrate. Biochem Int 19: 855–861, 1989

    Google Scholar 

  28. Shakir KMM, Sundaram SG, Margolis S: Lipid synthesis in isolated intestinal cells. J Lipid Res 19: 433–442, 1978

    Google Scholar 

  29. Gebhard RL, Ewing SL, Schlasner LA, Hunningmake DB, Prigge WF: Effect of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibition on human gut mucosa. Lipids 26: 492–494, 1991

    Google Scholar 

  30. Leighton B, Nicholas AR, Pogson CI: The pathway of ketogenesis in rumen epithelium of the sheep. Biochem J 216: 769–772, 1983

    Google Scholar 

  31. Day R, Middleton B: The major role of acetoacetyl-CoA thiolase in acetoacetate utilization confirmed in human cells. Biochem Soc Trans 17: 111–112, 1989

    Google Scholar 

  32. Gilbert HF, Lennox BJ, Mossman CD, Carle WC: The relation of acyl transfer to the overall reaction of thiolase I from porcine heart. J Biol Chem 256: 7371–7377, 1981

    Google Scholar 

  33. Veitch RK, Sherratt HSA, Causey AG, Bartlett K, Middleton B: The mechanism of mitochondrial fatty acid oxidation by mercaptoacetate: inhibition of acetoacetyl-CoA, 2-methylaceto acetyl-CoA and 3-oxoacyl-CoA thiolases. Biochem Soc Trans 14: 703–704, 1986

    Google Scholar 

  34. Azad Khan AK, Howes DT, Piris J, Truelove SC: Optimum dose of sulphasalazine for maintenance treatment in ulcerative colitis. Gut 21: 232–240, 1980

    Google Scholar 

  35. Allgayer H, Ahnfelt NO, Kruis W, Klotz U, Frank-Holmberg K, Soderberg HNA, Paumgartner G. Colonic N-acetylation of 5aminosalicylic acid in inflammatory bowel disease. Gastroenterology 97: 38–41, 1989

    Google Scholar 

  36. Ireland A, Priddle JD, Jewell DP: Acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells. Clinical Science 78: 105–111, 1990

    Google Scholar 

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Authors and Affiliations

  1. Cell Physiology Laboratory of the University of Adelaide Department of Surgery, Queen Elizabeth Hospital, 5011, Adelaide, S.A., Australia

    W. E. W. Roediger, O. Kapaniris & S. Millard

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  1. W. E. W. Roediger
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  2. O. Kapaniris
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  3. S. Millard
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Roediger, W.E.W., Kapaniris, O. & Millard, S. Lipogenesis from n-butyrate in colonocytes. Mol Cell Biochem 116, 113–118 (1992). https://doi.org/10.1007/BF00299390

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  • DOI: https://doi.org/10.1007/BF00299390

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