Skip to main content
Log in

Determination of degree of deacetylation of chitosan by 1H NMR spectroscopy

  • Published:
Polymer Bulletin Aims and scope Submit manuscript


This paper describes a novel method to determine the degree of deacetylation of chitosan by 1H NMR spectroscopy. Measurements were carried out at 70°C by using 2 wt% CD3COOD/D2O and 2 wt% DC1/D2O as solvents for chitosan. In the case of DC1/D2O system, effect on hydrolysis of chitosan should be taken into consideration, and the pulse repetition delay required for 45° pulse is 40s. Whereas, in regard to CD3COOD/D2O system, 6s pulse repetition delay is enough for 45° pulse, and moreover, high magnetic field NMR apparatus is necessary, because signal of CD2H residue in CD3COOD overlaps with that of CH3 residue in N-acetyl residue and determination of the difference of two spectra is required. The proposed method is more effective, precise and simple, comparing with the conventional colloid titration and elemental analysis methods. Assignments of 1H and 13C NMR spectra of chitosan are also reported.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others


  1. Terayama H (1952) J Polym Sci 8: 243

    Google Scholar 

  2. Sannan T, Kurita K, Ogura K, Iwakura Y (1978) Polymer 19: 458

    Google Scholar 

  3. Miya M, Iwamoto R, Yoshikawa S, Mima S (1980) Int J Biol Macromol 2: 323

    Google Scholar 

  4. Bax A (1982) Two-Dimensional Nuclear Magnetic Resonance in Liquids. Delft University Press, Holland.

    Google Scholar 

  5. Maudsley A-A, Ernst R-R (1977) Chem Phys Lett 50: 368

    Google Scholar 

  6. Bax A, Morris G-A (1981) J Magn Reson 44: 501

    Google Scholar 

  7. Domard A, Gey C, Rinaudo M, Terrassin C (1987) Int J Biol Macromol 9: 233

    Google Scholar 

  8. Usui T, Yamaoka N, Matsuda K, Tuzimura K, Sugiyama H, Seto S (1973) J Chem Soc Perkin Trans I: 2425

    Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and permissions

About this article

Cite this article

Hirai, A., Odani, H. & Nakajima, A. Determination of degree of deacetylation of chitosan by 1H NMR spectroscopy. Polymer Bulletin 26, 87–94 (1991).

Download citation

  • Accepted:

  • Issue Date:

  • DOI: