Summary
A region of the genome of the cyanobacterium Synechococcus R2, that bears a cluster of genes involved in nitrate assimilation, has been cloned and the relative positions of some of the genes in the region have been determined. Mutations generated by insertion of an antibiotic-resistance gene cassette into the gene encoding nitrite reductase are associated with reduced expression of nitrate reductase; cotranscription of nitrate assimilation genes in the cluster is inferred from this finding.
Similar content being viewed by others
References
Andriesse X, Bakker H, Weisbeek P (1990) Analysis of nitrate reduction genes in cyanobacteria. In: Ullrich WR, Rigano C, Fuggi A, Aparicio PJ (eds) Inorganic nitrogen in plants and microorganisms. Springer, Berlin, pp 303–307
Ausubel FA, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) (1990) Current protocols in molecular biology. Greene Publishing and Wiley-Interscience, New York
Buikema WJ, Haselkorn R (1991) Isolation and complementation of nitrogen fixation mutants of the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol 173:1879–1885
Elhai JE, Wolk CP (1988) A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68:119–138
Flores E, Ramos JL, Herrero A, Guerrero MG (1983) Nitrate assimilation by cyanobacteria. In: Papageorgiu GC, Packer L (eds) Photosynthetic prokaryotes: cell differentiation and function. Elsevier, New York, pp 363–387
Golden SS, Sherman LA (1984) Optimal conditions for genetic transformation of the cyanobacterium Anacystis nidulans R2. J Bacteriol 158:36–42
Herrero A, Guerrero MG (1986) Regulation of nitrite reductase in the cyanobacterium Anacystis nidulans. J Gen Microbiol 132:2463–2468
Herrero A, Flores E, Guerrero MG (1981) Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp strain 7119, and Nostoc sp strain 6719. J Bacteriol 145:175–180
Kuhlemeier CJ, Logtenberg T, Stoorvogel W, Heugten HAA, Borrias WE, van Arkel GA (1984a) Cloning of nitrate reductase genes from the cyanobacterium Anacystis nidulans. J Bacteriol 159:36–41
Kuhlemeier CJ, Teeuwsen WJP, Janssen MJT, van Arkel GA (1984b) Cloning of a thir nitrate reductase gene from the cyanobacterium Anacystis nidulans R2 using a shuttle gene library. Gene 31:109–116
Madueño F, Borrias WE, van Arkel GA, Guerrero MG (1988a) Isolation and characterization of Anacystis nidulans R2 mutants affected in nitrate assimilation: establishment of two new mutant types. Mol Gen Genet 213:223–228
Madueño F, Vega-Palas MA, Flores E, Herrero A (1988b) A cytoplasmic-membrane protein repressible by ammonium in Synechococcus R2: altered expression in nitrate-assimilation mutants. FEBS Lett 239:289–291
Meeks JC, Wolk CP, Lockau W, Schilling N, Shaffer PW, Chien W-S (1978) Pathways of assimilation of [13N]N2 and 13NH4 + by cyanobacteria with and without heterocysts. J Bacteriol 134:125–130
Omata T (1991) Cloning and characterization of the nrtA gene that encodes a 45-kDa protein involved in nitrate transport in the cyanobacterium Synechocococcus PCC 7942. Plant Cell Physiol 32:151–157
Omata T, Ohmori M, Arai N, Ogawa T (1989) Genetically engineered mutant of the cyanobacterium Synechococcus PCC 7942 defective in nitrate transport. Proc Natl Acad Sci USA 86:6612–6616
van den Hondel CAMJJ, Verbeek S, van der Ende A, Weisbeek PJ, Borrias WE, van Arkel GA (1980) Introduction of transposon Tn901 into a plasmid of Anacystis nidulans: preparation for cloning in cyanobacteria. Proc Natl Acad Sci USA 77:1570–1574
Vega-Palas MA, Madueño F, Herrero A, Flores E (1990) Identification and cloning of a regulatory gene for nitrogen assimilation in the cyanobacterium Synechococcus sp strain PCC 7942. J Bacteriol 172:643–647
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Luque, I., Herrero, A., Flores, E. et al. Clustering of genes involved in nitrate assimilation in the cyanobacterium Synechococcus . Molec. Gen. Genet. 232, 7–11 (1992). https://doi.org/10.1007/BF00299130
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00299130