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Effects of interleukin-1, tumor necrosis factor-β, and forskolin on tissue plasminogen activator activity in human osteoblastic osteosarcoma cells

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Summary

The effects of interleukin-1 (IL-1), forskolin, and tumor necrosis factor β (TNF-β) on tissue plasminogen activator (t-PA) activity were studied in the human osteoblastic osteosarcoma cell line, G292. t-PA activity was measured in the cell media using the chromogenic substrate, S-2251. After a 24 hour incubation period, IL-1 increased t-PA in a dose-dependent manner. The effect of IL-1 at 10.0 U/ml was partially inhibited in the presence of indomethacin. Forskolin (1.0 μM) increased t-PA activity after 24 hours with the effects of combined treatment of IL-1 (1.0 U/ml, 10.0 U/ml) and forskolin being apparently additive in nature. TNF-β (10-8–10-7 M) also produced increased t-PA activity in the cell medial after a 24 hour incubation period. These results suggest that the cytokines, IL-1 and TNF-β, can increase t-PA activity in G292 cells and that there is both a cAMP-dependent as well as a cAMP-independent pathway involved in the regulation of this osteoblastic cell function.

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Authors and Affiliations

  1. Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, 14214, Buffalo, New York, U.S.A.

    A. C. Kohl, D. N. Tatakis, C. Hansen & R. Dziak

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  1. A. C. Kohl
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  2. D. N. Tatakis
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  3. C. Hansen
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  4. R. Dziak
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Kohl, A.C., Tatakis, D.N., Hansen, C. et al. Effects of interleukin-1, tumor necrosis factor-β, and forskolin on tissue plasminogen activator activity in human osteoblastic osteosarcoma cells. Calcif Tissue Int 50, 129–133 (1992). https://doi.org/10.1007/BF00298789

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  • DOI: https://doi.org/10.1007/BF00298789

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