Skip to main content
SpringerLink
Log in

Ultrastructural and cytochemical investigations on the renal appendages and their concrements in dibranchiate cephalopods (Mollusea, Cephalopoda)

  • Published:
Zeitschrift für Morphologie der Tiere Aims and scope Submit manuscript

Summary

The renal appendages of the octopods Octopus vulgaris Lam., Eledone moschata Lam. and of the decapods of the order Sepioidea Sepia officinalis L., and of sea water suggest osmotic filtration combined with active ion transport and methods of light and electron microscopy and of cytochemistry. The cells of the excretory epithelium are polarised; in all species, they show a basal labyrinth with a thick positive PAS and alcian blue reaction. The cells contain numerous mitochondria, which show the crista type of internal structure, and in the basal as well as in the apical part there are lysosomal dense bodies with high phosphatase activity. The fine structure, the osmolality and the ionic composition of the blood, the urine and of sea water suggest osmotic filtration combined with active ion transport and apocrine excretory secretion and reabsorption. In Sepia only, crypts of the epithelium were found to contain spherical mixed crystals which contain Ca, K, Na, Mg, Cl, S, P according to the electron probe and cytochemical analysis; the matrix shows concentric layers, it gives a positive PAS and alcian blue reaction. The chemical composition, the genesis and the possible function of these crystals are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bargmann, W., von Hehn, G.: Über das Nephron der Elasmobranchier. Z.Zellforsch. 114,1–21 (1971)

    Google Scholar 

  • Becker, G. L., Chen, C. H., Greenawalt, I. W., Lehninger, A. C.: Calcium-phosphat granules in the hepatopancreas of the blue crab Callinectus sapidus. J. Cell Biol. 61, 316–326 (1974)

    Google Scholar 

  • Berridge, M., Oschman, J. L.: Transporting epithelia. New York-London: Academic Press 1972

    Google Scholar 

  • von Boletzky, S.: Untersuchungen über die Ontogenese des Kreislaufsystems von Octopus vulgaris Lam. Rev. Suisse Zool. 75, 765–812 (1968)

    Google Scholar 

  • von Boletzky, S.: Effets de la sous-nutrition prolongée sur le développement de la coquille de Sepia officinalis L. (Mollusca, Cephalopoda). Bull. Soc. Zool. France 99, 667–673 (1974)

    Google Scholar 

  • Boucaud-Camou, E.: Étude infrastructurale du pancréas de Sepia officinalis L. Bull. Sec. Zool. France, 97, 197–203 (1972)

    Google Scholar 

  • Carasso, N., Favard, P.: Mise en évidence du calcium dans les myonèmes pédonuclaires de ciliés péritriches. J. Microsc. 5, 759–770 (1966)

    Google Scholar 

  • Delaunay, H.: L'exeretion azotée des invertébrés. Biol. Rev. 6, 265–301 (1931)

    Google Scholar 

  • Eichelberg, D., Wessing, A.: Elektronenoptische Untersuehungen an den Nierentubuli (Malpighische Gefäße von Drosophila melanogaster. II. Transzelluläre membrangebundene Stofftransportmechanismen. Z. Zellforsch. 121, 127–152 (1971)

    Google Scholar 

  • Emmanuel, C. F.: The composition of Octopus renal fluid. II. Organic constituents. Z. vergl. Physiol. 39, 477–482 (1956)

    Google Scholar 

  • Emmanuel, C. F.: The composition of Octopus renal fluid. III. The isolation and chemical properties of Dicyemin. Z. vergl. Physiol. 39, 483–491 (1957a)

    Google Scholar 

  • Emmanuel, C. F.: The composition of Octopus renal fluid. IV. Isolation and identification of the methanol soluble substances. Z. vergl. Physiol. 40, 1–7 (1957b)

    Google Scholar 

  • Emmanuel, C. F., Martin, A. W.: The composition of Octopus renal fluid. I. The inorganic constituents. Z. vergl. Physiol. 39, 226–234 (1956)

    Google Scholar 

  • Ericsson, J. L. E., Olsen, S.: On the fine structure of the aglomerular renal tubule in Lophius piscatorius. Z. Zellforsch. 104, 240–258 (1970)

    Google Scholar 

  • Fioroni, P., Meister, G.: Embryologie von Loligo vulgaris Lam. In: Gr. Zool. Praktikum. Stuttgart: Fischer 1974

    Google Scholar 

  • Grobben, C.: Morphologische Studien über den Harn- und Geschlechtsapparat sowie die Leibeshöhle der Cephalopoden. Arb. aus dem zool. Inst. der Univ. Wien und der Zool. Station Triest 5, 179–252 (1883)

    Google Scholar 

  • Harrison, F. M., Martin, A. W.: Excretion in the cephalopod Octopus dofleini. J. exp. Biol. 42, 71–98 (1965)

    Google Scholar 

  • Hevert, F., Wolburg, H., Wessing, A.: Die Konkremente des larvalen Primäharns von Drosophila hydei. II. Anorganische Bestandteile. Cytobiologie 8, 312–319 (1974)

    Google Scholar 

  • Istin, M., Girard, J. P.: Carbonic anhydrase and mobilisation of calcium reserves in the mantle of lamellibranchs. Calcif. Tiss. Res. 5, 247–260 (1970)

    Google Scholar 

  • Komnick, H.: Histochemische Calcium-Lokalisation in der Skelettmuskulatur des Frosches. Histochemie 18, 24–29 (1969)

    Google Scholar 

  • Marthy, H. J.: Die Organogenese des Coelomsystems von Octopus vulgaris. Rev. Suisse Zool. 75, 723–763 (1968)

    Google Scholar 

  • Meister, G.: Organogenese von Loligo vulgaris Lam. Zool. J. b. Anat. 89, 247–300 (1972)

    Google Scholar 

  • Naef, A.: Die Organogenese des Cölomsystems und der zentralen Blutgefäße von Loligo. Jena. Z. Med. Naturw. 45, 1–46 (1909)

    Google Scholar 

  • Naef, A.: Teutologische Notizen XI: Zur Morphologic des Cölomsystems. Zool. Anz. 40, 324–336 (1912)

    Google Scholar 

  • Naef, A.: Die Cephalopoden. In: Fauna und Flora des Golfes von Neapel, Berlin (1923)

  • Olsen, S., Ericsson, J. L. E.: Ultrastructure of the tubule of the aglomerular teleost Nerophis ophidion. Z. Zellforsch. 87, 17–30 (1968)

    Google Scholar 

  • Potts, W. T. W.: Ammonia excretion in Octopus dofleini. Comp. Biochem. Physiol. 14, 339–355 (1965)

    Google Scholar 

  • Potts, W. T. W.: Excretion in the molluscs. Biol. Rev. 42, 1–42 (1967)

    Google Scholar 

  • Potts, W. T. W., Todd, M.: Kidney function in Octopus. Comp. Biochem. Physiol. 16, 479–489 (1965)

    Google Scholar 

  • Riegel, J. A.: In vitro studies of fluid and ion movements due to the swelling of formed bodies. Comp. Biochem. Physiol. 35, 843–856 (1970a)

    Google Scholar 

  • Riegel, J. A.: A new model of transepithelial fluid movement with detailed application to fluid movement in the crayfish antennal gland. Comp. Biochem. Physiol. 36, 403–410 (1970b)

    Google Scholar 

  • Robertson, J. D.: Further studies on ionic regulation in marine invertebrates. J. exp. Biol. 30, 277–296 (1953)

    Google Scholar 

  • Roinel, N., Morel, F., Istin, M.: Étude des granules calcifiés du manteau des lamellibranches à l'aide de la microsonde éctronique. Calcif. Tiss. Res. 11, 163–170 (1973)

    Google Scholar 

  • Schipp, R., von Boletzky, S.: Morphology and function of the excretory organs in dibranchiate cephalopods. 3. International Symposium of the “Akademie der Wissenschaften und Literatur”. Mainz 1974 — Excretion. In: Fortschr. Zool. 23, 89–110 (1975)

    Google Scholar 

  • Schipp, R., Höhn, P., Schafer, A.: Elektronenmikroskopische und histochemische Untersuchungen zur Funktion des Kiemenherzanhanges (Pericardialdrüse) von Sepia of ficinalis. Z. Zellforsch. 117, 252–274 (1971)

    Google Scholar 

  • Schipp, R., Schäfer, A.: Vergleichende elektronenmikroskopische Untersuchungen an den zentralen Herzorganen der Cephalopoden (Sepia officinalis). Feinstruktur und Funktion der Kiemenherzen. Z. Zellforsch. 101, 367–379 (1969)

    Google Scholar 

  • Tice, L. W., Barnett, R. J.: Alcianblue staining for electron microscopy. J. Histochem. Cytochem. 10, 688–689 (1962)

    Google Scholar 

  • Vigelius, W. J.: Über das Exkretionssystem der Cephalopoden. Niederländ. Arch. Zool. 5, 115–184 (1880)

    Google Scholar 

  • Wessing, A.: Die Funktion der MalpighischenGefäße. In: Funktionelleund morphologische Organisation der Zelle. Sekretion und Exkretion. Berlin-Heidelberg-New York: Springer 1965

    Google Scholar 

  • Wessing, A., Eichelberg, D.: Morphology of the transport and accumulation of substances in the Malpighian tubules of Drosophila. 3. International Symposium of the “Akademie der Wissenschaften and Literatur”. Mainz 1974—Excretion. In: Fortschr. Zool. (in press)

  • Wolburg, H., Hevert, F., Wessing, A., Porstendoerfer, J.: Die Konkremente des larvalen Primärharns von Drosophila hydei. I. Struktur. Cytobiologie 8, 25–38 (1973)

    Google Scholar 

  • Yokota, S.: Electron microscopic demonstration of carbonic anhydrase activity in mouse liver cells. Histochemie 19, 255–261 (1969)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. I. Zoologisches Institut der Justus Liebig-Universität, Stephanstr. 24, D-6300, Giessen, Germany

    Rudolf Schipp,  Sigurd von Boletzky & Gerd Doell

  2. Laboratoire Arago, Université de Paris VI, F-66650, Banyuls-sur-Mer, France

    Gerd Doell

Authors
  1. Rudolf Schipp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sigurd von Boletzky
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gerd Doell
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Animals were collected and prepared at the Station biologique d'Arcachon and the Laboratoire Arago, Banyuls-sur-Mer (France). This study was supported by the Deutsche Forschungsgemeinschaft.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schipp, R., von Boletzky, S. & Doell, G. Ultrastructural and cytochemical investigations on the renal appendages and their concrements in dibranchiate cephalopods (Mollusea, Cephalopoda). Z. Morph. Tiere 81, 279–304 (1975). https://doi.org/10.1007/BF00298489

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00298489

Keywords

Search

Navigation