Kin preference behavior in Bufo boreas tadpoles

Summary

The ontogeny of sibling recognition behavior was studied in the laboratory in tadpoles of the western toad (Bufo boreas boreas) to test the hypothesis that Bufo tadpoles associate with siblings and to compare this behavior with two species of anurans previously studied. Tadpoles reared exclusively with sibs demonstrated a preference to associate with sibs over non-sibs both early and late in development but tadpoles reared with sibs and non-sibs (mixed rearing groups) exhibited no preference. Larvae that developed a preference for sibs after being reared with them for 75 days lost this preference following exposure to a mixed group for 2 to 6 days. Additionally, larvae reared in a mixed group did not develop a preference for the familiar mixed group nor was a preference exhibited by individual larvae that were reared exclusively with 5 non-sibs. These results suggest that larvae learn by prior association to discriminate sibs from non-sibs and that preferences are rapidly modifiable following exposure to non-sibs. However, the latter two experiments suggest that social preferences are not totally labile and are not based entirely on familiarity. Field observations of larval activity, aggregation behavior, and dispersal patterns in B. boreas indicate that tadpoles mix with non-sibs from early larval stages throughout development. Because even short term exposure to nonsibs resulted in a loss of sib preference in our experiments, we suggest that a ‘kin selection’ interpretation of B. boreas aggregation behavior is not parsimonious. Results differ with those of other studies of sib recognition in larval anurans.