Summary
This paper presents data on the pattern obtained by labelling in vitro with 3H-AMD human chromosomes from peripheral blood cultures. Preparations were divided into two experimental groups: 1. preparations labelled with 3H-AMD; 2. preparations treated with H2SO4 and labelled with 3H-AMD.
The results show evidence of non-random grain distribution for some chromosomes and of differential intensity of labelling of chromosomes within some chromosome groups. Treatment with H2SO4 before labelling with 3H-AMD resulted in random distribution of grains along the chromosomes with disappearance of any labelling pattern. The same result was observed when preparations were stained with quinacrine dihydrochloride before labelling with 3H-AMD. These data suggest the existence of a differential distribution of proteins along the chromosomes.
Zusammenfassung
In der vorliegenden Arbeit wird die in vitro-Markierung von menschlichen Chromosomen mit 3H-Aktinomycin D (AMD) in Kulturen des peripheren Blutes beschrieben. Es handelt sich um zwei Versuchsgruppen: 1. Markierung der Präparationen mit 3H-AMD; 2. Markierung der Präparationen mit 3H-AMD nach Vorbehandlung mit Schwefelsäure.
Bei diesen Versuchen ergaben sich Hinweise auf eine nichtzufallsmäßige Kornverteilung auf einigen Chromosomen und auf differentielle Markierungsmuster in den Chromosomen einiger Chromosomengruppen. Diese Markierungsmuster wurden durch Vorbehandlung mit Schwefelsäure zerstört. Das gleiche Resultat wurde erhalten, wenn die Präparate vor der Markierung mit Chinakrin-Dihydrochlorid gefärbt wurden. Diese Ergebnisse lassen vermuten, daß die Proteine auf den Chromosomen ungleichmäßig verteilt sind.
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Rocchi, A., Gigliani, F., de Capoa, A. et al. Labelling of human chromosomes with 3H-AMD. Hum Genet 24, 297–301 (1974). https://doi.org/10.1007/BF00297593
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DOI: https://doi.org/10.1007/BF00297593