Summary
Parathyroid hormone (PTH) binds specifically to the hypertrophic region of growth plate cartilage [16]. This specific binding suggests a role for this hormone in chondrocyte maturation. Enzymatically isolated chick articular and growth plate chondrocytes grown in monolayer culture were used to assay the direct effects of PTH on chondrocytes. The articular chondrocytes were unresponsive to PTH. The growth plate chondrocytes, however, demonstrated a marked mitogenic response to PTH, with a 39-fold increase of [3H]-thymidine incorporation into DNA. PTH also affected matrix production by the growth plate chondrocytes causing a twofold stimulation of proteoglycan synthesis as determined by the rate of 35SO4 incorporated into matrix macromolecules. Furthermore, PTH depressed collagen synthesis as measured by [3H]-proline incorporation. PTH caused a 12-fold increase in intracellular cAMP in growth plate chondrocytes but no increase in the articular cells. This specificity of PTH for growth plate chondrocytes suggests a possible regulatory role in enchondral ossification.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Poole RA (1982) Physiology of cartilage formation, function, and festruction. In: Creuss RL (ed) The musculoskeletal system: Embryology, biochemistry, and physiology. Churchill Livingstone, New York, Edinburgh, London, Melbourne, pp 191–217
Boskey A (1981) Current concepts in the physiology and biochemistry of calcification. Clin Orthop 157:225–257
Wuthier RE (1982) A review of the primary mechanism of endochondral calcification with special emphasis on the role of cells, mitochondria and matrix vesicles. Clin Orthop 169:219–242
Mankin HJ (1962) Localization of tritiated thymidine in articular cartilage of rabbits. J Bone Joint Surg 44a:682–688
Mankin HJ (1963) Localization of tritiated thymidine in articular cartilage of rabbits. J Bone Joint Surg 45a:529–540
Muir IHM (1979) The chondrocytes. In: Freeman MAR (ed) Adult articular cartilage. Pitman Medical Publishing Co, England, pp 145–214
Einhorn TE, Gordon SL, Siegel SA, Hummel CF, Avitable MJ, Carty RP (1985) Matrix vesicle enzymes in human osteoarthitis. J Ortho Res 3:160–169
Silberberg M, Silberberg R (1943) The effects of parathyroid hormone and calcium gluconate on the skeletal tissues of mice. Am J Pathol 19:839–859
Smith DM, Roth LM, Johnston CC (1976) Hormonal responsiveness of adenylate cyclase activity in cartilage. Endocrinology 98:242–246
Takigawa M, Takano T, Suzuki F (1981) Effects of parathyroid hormone and cyclic AMP analogues on the activity of ornithine decarboxylase and expression of the differentiated phenotype of chondrocytes in culture. J Cell Physiol 106:259–268
Takigawa M, Hiroshi I, Takano T, Suzuki F (1982) Polyamines and differentiation: induction of ornithine decarboxylase by parathyroid hormone is a good marker of differentiated chondrocytes. Proc Natl Acad Sci USA 77:1481–1485
Takigawa M, Takano T, Suzuki F (1983) Role of polyamines in expression of the differentiated phenotype of chondrocytes: effect of DL-a-Hydrazino-d-Aminovaleric Acid (DL-HAVA), an inhibitor of ornithine decarboxylase, on chondrocytes treated with parathyroid hormone. J Biochem 93:591–598
Hiraki Y, Yutani Y, Takigawa M, Kato Y, Suzuki F (1985) Differential effects of parathyroid hormone and somatomedin-like growth factors on the sizes of proteoglycan monomers and their synthesis in rabbit costal chondrocytes in culture. Biochim Biophys Acta 845:445–453
Deshmukh K, Kline WG, Sawyer BD (1976) Role of calcium in the phenotypic expression of rabbit articular chondrocytes in culture. FEBS Letters 67:48–51
Deshmukh K, Kline WG, Sawyer BD (1977) Effects of calcitonin and parathyroid hormone on the metabolism of chondrocytes in culture. Biochim Biophys Acta 499:28–35
Barling PM, Bibby NJ (1985) Study of the localization of [3H]bovine parathyroid hormone in bone by light microscope autoradiography. Calcif Tissue Int 37:441–446
O'Keefe RJ, Puzas JE, Rosier RN (1988) Effect of transforming growth factor-β on DNA synthesis by growth plate chondrocytes: modulation by factors in serum. Calcif Tissue Int 43:352–358
O'Keefe RJ, Puzas JE, Rosier RN (1988) Effects of transforming growth factor-β on matrix synthesis by chick growth plate chondrocytes. Endocrinology 122:2953–2961
Northington F, Hamill R, Banergee S (1985) Dopaminestimulated adenylate cyclase and tyrosine hydroxylase in diabetic rat retina. Brain Res 337:151–159
Menczel J, Robey RG (1986) Factors affecting cAMP production by human bone cells in vitro. J Bone Miner Res 1:155
Crabb ID, O'Keefe RJ, Puzas JE, Rosier RN (1990) Synergistic effects of transforming growth factor beta and fibroblast growth factor on chick growth plate chondrocytes. J Bone Miner Res 5:1105–1112
Puzas JE, Brand JS (1986) The effect of bone cell stimulatory factors can be measured only under specific conditions. Calcif Tissue Int 39:104–108
Bronner F (1961) Parathyroid effects on sulfate metabolism: interrelations with calcium. In: Greep RO and Talmage RV (eds). The parathyroids. Charles C. Thomas, Springfield, Illinois
Kuhlman RE (1965) Phosphatases in epiphyseal cartilage: their possible role in tissue synthesis. J Bone Joint Surg 47-A:545–550
Lewinson D, Toister Z, Silbermann M (1982) Quantitative and distributional changes in the activity of alkaline phosphatase during the maturation of cartilage. J Histochem Cytochem 30:261–269
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Crabb, I.D., O'Keefe, R.J., Puzas, J.E. et al. Differential effects of parathyroid hormone on chick growth plate and articular chondrocytes. Calcif Tissue Int 50, 61–66 (1992). https://doi.org/10.1007/BF00297299
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00297299