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Involvement of the PS03 gene of Saccharomyces cerevisiae in intrachromosomal mitotic recombination and gene amplification

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Abstract

Using a genetic system of haploid strains of Saccharomyces cerevisiae carrying a duplication of the his4 region on chromosome III, the pso3-1 mutation was shown to decrease the rate of spontaneous mitotic intrachromosomal recombination 2- to 13-fold. As previously found for the rad52-1 mutant, the pso3-1 mutant is specifically affected in mitotic gene conversion. Moreover, both mutations reduce the frequency of spontaneous recombination. However, the two mutations differ in the extent to which they affect recombination between either proximally or distally located markers on the two his4 heteroalleles. In addition, amplifications of the his4 region were detected in the pso3-1 mutant. We suggest that the appearance of these amplifications is a consequence of the inability of the pso3-1 mutant to perform mitotic gene conversion.

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Author notes

  1. Lisiane B. Meira

    Present address: Laboratório de Radiobiología Molecular, Centro de Biotecnologia, UFRGS, Av. Bento Gongalves 9500, 91501-970, Porto Alegre, RS, Brazil

Authors and Affiliations

  1. Section Biologic, URA 1292 CNRS, Institut Curie, 26 rue d'Ulm, F-75231, Paris Cedex 05, France

    Lisiane B. Meira, Nieve Magaña-Schwencke & Dietrich Averbeck

  2. Departamento de Biohsica, Centro de Biotecnologia, UFRGS, Av. Bento Gongalves 9500, 91501-970, Porto Alegre, RS, Brazil

    Joâo Antonio P. Henriques

Authors
  1. Lisiane B. Meira
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  2. Nieve Magaña-Schwencke
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  3. Dietrich Averbeck
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  4. Joâo Antonio P. Henriques
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Communicated by R. Devoret

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Meira, L.B., Magaña-Schwencke, N., Averbeck, D. et al. Involvement of the PS03 gene of Saccharomyces cerevisiae in intrachromosomal mitotic recombination and gene amplification. Molec. Gen. Genet. 245, 750–759 (1994). https://doi.org/10.1007/BF00297282

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  • DOI: https://doi.org/10.1007/BF00297282

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