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Expression of human poly(ADP-ribose) polymerase in Saccharomyces cerevisiae

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Abstract

The coding sequence for human poly(ADP-ribose) polymerase was expressed inducibly in Saccharomyces cerevisiae from a low-copy-number plasmid vector. Cell free extracts of induced cells had poly(ADPribose) polymerase activity when assayed under standard conditions; activity could not be detected in non-induced cell extracts. Induced cells formed poly(ADP-ribose) in vivo, and levels of these polymers increased when cells were treated with the alkylating agent N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). The cytotoxicity of this agent was increased in induced cells, and in vivo labelling with [3H]adenine further decreased their viability. Increased levels of poly(ADP-ribose) found in cells treated with the alkylating agent were not accompanied by lowering of the NAD concentration.

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  1. Institute of Pharmacology and Biochemistry, University of Zurich, Tierspital, Winterthurerstrasse 260, 8057, Zürich, Switzerland

    M. A. Collinge & F. R. Althaus

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  1. M. A. Collinge
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  2. F. R. Althaus
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Collinge, M.A., Althaus, F.R. Expression of human poly(ADP-ribose) polymerase in Saccharomyces cerevisiae . Molec. Gen. Genet. 245, 686–693 (1994). https://doi.org/10.1007/BF00297275

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  • DOI: https://doi.org/10.1007/BF00297275

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