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Non-homogeneous Ca release in isolated frog skeletal muscle fibres

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Summary

We have examined the spatial distribution of [Ca2+]i during tetanic stimulation in frog skeletal muscle cells using a fluorescence imaging method. We have found a completely unexpected pattern of Ca release: Ca is released forming gradients composed of spots of very significant and slow fluctuations of calcium release. Our findings could be explained if the calcium release process in skeletal muscle is influenced significantly by [Ca2+]i, such as in cardiac muscle, and suggests that the SR/Ca release control can include the established voltage-dependent plus a cardiac-like process of calcium-induced Ca release and a Ca release inhibition by Ca.

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Authors and Affiliations

  1. Department of Biophysics, University of Maryland School of Medicine, 21201, Baltimore, MD, USA

    Monika Rozycka & Hugo Gonzalez-Serratos

  2. Department of Physiology, University of Maryland School of Medicine, 21201, Baltimore, MD, USA

    William Goldman

  3. Geriatric Research Education and Clinical Center, Baltimore VA Medical Center, 21201, Baltimore, MD, USA

    William Goldman

Authors
  1. Monika Rozycka
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  2. Hugo Gonzalez-Serratos
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  3. William Goldman
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Rozycka, M., Gonzalez-Serratos, H. & Goldman, W. Non-homogeneous Ca release in isolated frog skeletal muscle fibres. J Muscle Res Cell Motil 14, 527–532 (1993). https://doi.org/10.1007/BF00297215

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  • DOI: https://doi.org/10.1007/BF00297215

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