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Gene amplifications in advanced-stage human prostate cancer

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Abstract

Gene amplification is a model of proto-oncogene alterations occasionally observed in human tumors. This amplification can, in some cases, have prognostic value (N-myc in neuroblastoma, c-erbB2 and int-2 in breast cancer, etc.). Amplifications of the proto-oncogenes c-myc, c-erbB2 and int-2 have not yet been report in prostate adenocarcinoma, which, like breast cancer, is hormone dependent. We sought amplifications of these three proto-oncogenes by means of Southern blotting in 15 human prostate adenocarcinoma specimens, most of which were advanced (7 stage C and 6 stage D1 or D2). We confirmed the lack of c-myc and c-erbB2 amplification, regardless of the stage, in contrast to the case of breast cancer. Int-2 amplification was observed in one advanced tumor with bone metastases, out of a total of six stage D tumors. The precise frequency of int-2 amplification and its role in prostate carcinogenesis remain to be determined.

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References

  1. Ali IU, Merlo G, Callahan R, Lidereau R (1989) The amplification unit on chromosome 11q13 in aggressive primary human breast tumors entails the bcl1, int2, hst loci. Oncogene 4:89

    Google Scholar 

  2. Anwar K, Nakakuki K, Shiraishi T, Naiki H, Yatani R, Inuzuka M (1992) Presence of ras oncogene mutations and human papillomavirus DNA in human prostate carcinomas. Cancer Res 52:5991–5996

    Google Scholar 

  3. Brookes S, Lammie GA, Shuuring E, de Boer C, Michalides R, Dickson C, Peters G (1993) Amplified region of chromosome band 11q13 in breast and squamous cell carcinomas encompasses three CpG islands telomeric of FGF3, including the expressed gene EMS1. Genes Chromosom Cancer 6:222

    Google Scholar 

  4. Bussemakers MJ, Debruyne FM, Schalken JA (1992) Genetic steps associated with prostate cancer development. In: Olsson CA (ed) Oncogenes and molecular genetics of urological tumours (Société internationale d'urologie reports). Churchill Livingstone, Edinburgh, p 29

    Google Scholar 

  5. Buttyan R, Sawczuc IS, Benson MC, Siegal JD, Olsson CA (1987) Enhanced expression of the c-myc proto-oncogene in high grade human prostate cancers. Prostate 11:327

    Google Scholar 

  6. Carter HB, Piantadosi S, Isaacs JT (1990) Clinical evidence for and implications of the multistep development of prostate cancer. J Urol 143:742

    Google Scholar 

  7. Casey G, Smith R, McGillivray D, Peters G, Dickson C (1986) Characterization and chromosome assignment of the human homolog of int-2, a potential proto-oncogene. Mol Cell Biol 6(2):502

    Google Scholar 

  8. Coussens L, Yang-Fen TL, Liao YC, Chen E, Gray A, McGrath J, Seeburg PH, Libermann TA, Schlessinger J, Francke U, Levinson A, Ullrich A (1985) Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene. Science 230:1132

    Google Scholar 

  9. De Taisne T, Gegonne A, Stehelin D (1984) Chromosomal localization of the human proto-oncogene c-ets. Nature 310:581

    Google Scholar 

  10. Escot C, Theillet C, Lidereau R, Spyratos F, Champeme MH, Gest M, Callahan R (1986) Genetic alterations of the c-myc proto-oncogene in human primary breast carcinomas. Proc Natl Acad Sci USA 83:4834

    Google Scholar 

  11. Fan K (1988) Heterogeneous subpopulations of human prostatic adenocarcinoma cells: potential usefulness of p21 protein as a predictor for bone metastasis. J Urol 139:318

    Google Scholar 

  12. Fearon ER, Vogelstein B (1990) A genetic model for colorectal tumorigenesis. Cell 61:759

    Google Scholar 

  13. Fleming WH, Hamel A, MacDonald R, Ramsey E, Pettigrew NM, Johnston B, Dodd JG, Matusik RJ (1986) Expression of the c-myc proto-oncogene in human prostatic carcinoma and benign prostatic carcinoma and benign prostatic hyperplasia. Cancer Res 46:1535

    Google Scholar 

  14. Haenszel W, Kurihara M (1968) Studies of Japanese migrants: I. Mortality from cancer and other diseases among Japanese in the United States. JNCI 40:43

    Google Scholar 

  15. Johansson JE, Andersson SO, Krusemo UB, Adami HO, Bergström R, Kraaz W (1989) Natural history of localised prostate cancer: A population-based study in 223 untreated patients. Lancet I:799

    Google Scholar 

  16. Lidereau R, Callahan R, Dickson C, Peters G, Escot C, Ali IU (1988) Amplification of the int-2 gene in primary human breast tumors. Oncogene Res 2:285

    Google Scholar 

  17. Macoska JA, Powell IJ, Sakr W, Lane MA (1992) Loss of the 17p chromosomal region in a metastatic carcinoma of the prostate. J Urol 147:1142

    Google Scholar 

  18. Maniatis T, Fritsch EF, Sambook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1982

    Google Scholar 

  19. Mellon K, Thompson S, Charlton RG, Marsh C, Robinson M, Lane DP, Harris AL, Wilson-Horne CH, Neal DE (1992) P53, c-erbB-2 and the epidermal growth factor receptor in the benign and malignant prostate. J Urol 147:496

    Google Scholar 

  20. Motokura T, Bloom T, Kim HG, Juppner H, Ruderman JU, Kronenberg HM, Arnold A (1991) A novel cyclin encoded by a bcl-1 linked candidate oncogene. Nature 350:512

    Google Scholar 

  21. Moul JW, Friedrichs PA, Lance RS, Theune SM, Chang EH (1992) Infrequent Ras oncogene mutations in human prostate cancer. Prostate 20:327

    Google Scholar 

  22. Nag A, Smith RG (1989) Amplification, rearrangement and elevated expression of c-myc in the human prostatic carcinoma cell line LNCaP. Prostate 15:115

    Google Scholar 

  23. Rijnders AW, Van der Korput JA, Van Steenbrugge GJ, Romijn JC, Trapman J (1985) Expression of cellular oncogenes in human prostatic carcinoma cell lines. Biochem Biophys Res Commun 132:548

    Google Scholar 

  24. Rushdi A, Nishikura K, Erikson J, Watt R, Rovera G, Croce CM (1983) Differential expression of the translocated and untranslocated c-myc oncogene in Burkitt lymphoma. Science 222:390

    Google Scholar 

  25. Schuuring E, Verhoeven E, Mooi WJ, Michalides R (1992) Identification and cloning of two overexpressed genes, U21B31/PRAD1 and EMS1, within the amplified chromosome 11q13 region in human carcinomas. Oncogene 7:355

    Google Scholar 

  26. Seeger RC, Brodeur GM, Sather H, Dalton A, Siegel SE, Wong KY, Hammond D (1985) Association of multiple copies of the N-myc oncogene with rapid progression of neuroblastomas. Engl J Med 313:1111

    Google Scholar 

  27. Slamon DJ, Clark GM, Wong SG, Levin WJ, Ullrich A, MacGuire WL (1987) Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science 235:177

    Google Scholar 

  28. Somers KD, Cartwright SL, Schechter GL (1990) Amplification of the int-2 gene in human head and neck squamous cell carcinomas. Oncogene 5:915

    Google Scholar 

  29. Steinberg GD, Carter BS, Beaty TH, Childs B, Walsh PC (1990) Family history and the risk of prostate cancer. Prostate 17:337

    Google Scholar 

  30. Szepetowski P, Perucca-Lostanlen D, Gaudray P (1991) Amplification, proto-oncogènes et cancers humains. Med Sci 7:14

    Google Scholar 

  31. Thompson TC, Southgate J, Kitchener G, Land H (1989) Multistage carcinogenesis induced by ras and myc oncogenes in reconstituted organ. Cell 56:917

    Google Scholar 

  32. Treiger B, Isaacs J (1988) Expression of a transfected v-Harvey ras oncogene in a Dunning rat prostate adenocarcinoma and the development of high metastatic ability. J Urol 140:1580

    Google Scholar 

  33. Zhau HE, Wan DS, Zhou J, Miller GJ, Eschenbach AC von (1992) Expression of c-erbB-2/neu proto-oncogene in human prostatic cancer tissue and cell lines. Mol Carcinog 5:320

    Google Scholar 

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Authors and Affiliations

  1. Service d'Urologie, Hôpital Morvan, Brest, France

    G. Fournier & P. Mangin

  2. Service d'Onco-virologie, Centre René Huguenin, Saint Cloud, France

    A. Latil & R. Lidereau

  3. Service de Biochimie et de Biologic Moléculaire, Hopital Morvan, Brest, France

    Y. Amet, J. H. Abalain & H. H. Floch

  4. Service d'Anatomo-pathologie, Hôpital Morvan, Brest, France

    A. Volant

Authors
  1. G. Fournier
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  2. A. Latil
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  3. Y. Amet
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  4. J. H. Abalain
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  5. A. Volant
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  6. P. Mangin
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  7. H. H. Floch
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  8. R. Lidereau
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Fournier, G., Latil, A., Amet, Y. et al. Gene amplifications in advanced-stage human prostate cancer. Urol. Res. 22, 343–347 (1995). https://doi.org/10.1007/BF00296872

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  • DOI: https://doi.org/10.1007/BF00296872

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