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Coordinate inhibition of alkaline phosphatase and type X collagen syntheses by 1,25-dihydroxyvitamin D3 in primary cultured hypertrophic chondrocytes

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Summary

The effects of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (2.3×10-12-1.4×10-6 [M]) on alkaline phosphatase, collagen, and cell proliferation were examined in primary cultured hypertrophic chondrocytes prepared from the distal epiphyseal growth plate of the tibias of 12-day chick embryos. 1,25(OH)2D3 showed time- and dose-dependent inhibitory effects on the alkaline phosphatase and collagen levels. The inhibition of alkaline phosphatase activity became detectable at 2×10-11 [M] and reached 10% of control at 10-7 [M]. The concentration of 1,25(OH)2D3 giving a 50% inhibition of the enzyme level was approximately 3×10-10 [M]. Of the two extracellular collagen pools, a cell-associated matrix pool showed a more dramatic decrease (to 10% of control) than a culture medium pool (to 50% of control) at increased 1,25(OH)2D3 concentrations. The degree of inhibition was different for each type of chondrocyte-specific collagen (types II, IX, X, and XI). Types II and IX were inhibited in a parallel manner to only 60–80% of control. On the other hand, types X and XI were more greatly reduced up to 10% of control, and their dose-dependent inhibitory curves were similar to that of alkaline phosphatase. On cell proliferation, 1,25(OH)2D3 had a biphasic effect: stimulation at 10-10–10-8 [M] and inhibition at higher levels. The results revealed the significant involvement of 1,25(OH)2D3 in the metabolism of two probable calcification-related products, alkaline phosphatase and type X collagen.

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Authors and Affiliations

  1. Department of Biochemistry, University of Illinois, Urbana, Illinois, USA

    Shigenori Inao & H. Edward Conrad

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  1. Shigenori Inao
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  2. H. Edward Conrad
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Inao, S., Conrad, H.E. Coordinate inhibition of alkaline phosphatase and type X collagen syntheses by 1,25-dihydroxyvitamin D3 in primary cultured hypertrophic chondrocytes. Calcif Tissue Int 50, 445–450 (1992). https://doi.org/10.1007/BF00296776

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  • DOI: https://doi.org/10.1007/BF00296776

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