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Species differences in the immunophenotype of osteoclasts and mononuclear phagocytes

  • Laboratory Investigations
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Summary

Human osteoclasts, in contrast to mononuclear phagocytes, are known to express a well-defined restricted range of myeloid antigens. To determine whether these antigenic differences are present in other species, we examined the immunophenotype of chicken and rabbit ostoeclasts, macrophages, macrophage polykaryons, and monocytes and compared them with similarly derived and cultured human cells. Human, rabbit, and avian osteoclasts reacted with monoclonal antibodies against human β1 integrins (CD29, CD49b, CD49d), β3 integrins (CD51, CD61), as well as human macrophage-associated antigen CD68. Avian osteoclasts also reacted for CD11a/18 and CD14 which are not present on human osteoclasts. Avian and mammalian monocytes, macrophages, and macrophage polykaryons expressed all the above antigens. Both avian and human macrophage polykaryons produced by culture of peritoneal macrophages reacted with anti-CD51 antibodies indicating that expression of the vitronectin receptor alone does not distinguish between these cells in vitro.

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Authors and Affiliations

  1. Nuffield Department of Pathology, University of Oxford, John Radcliffe Hospital, OX3 9DU, Headington, Oxford, UK

    N. A. Athanasou & J. M. Quinn

  2. Department of Pathology and Laboratory Medicine, Jewish Hospital, Washington University School of Medicine, 63110, St. Louis, MO, USA

    J. I. Alvarez, F. P. Ross & S. L. Teitelbaum

Authors
  1. N. A. Athanasou
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  2. J. I. Alvarez
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  3. F. P. Ross
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  4. J. M. Quinn
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  5. S. L. Teitelbaum
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Athanasou, N.A., Alvarez, J.I., Ross, F.P. et al. Species differences in the immunophenotype of osteoclasts and mononuclear phagocytes. Calcif Tissue Int 50, 427–432 (1992). https://doi.org/10.1007/BF00296773

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  • DOI: https://doi.org/10.1007/BF00296773

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