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Expression of MIB-1, mitotic index and S-phase fraction as indicators of cell proliferation in suerficial bladder cancer

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Abstract

Cell proliferation of transitional cell bladder cancer (TCC) was determined by MIB-1 immunolabeling, volume-corrected mitotic index (M/V index) and S-phase fraction measurement in 207 patients with superficial (Ta-T1) bladder cancer. The results were compared to T category, WHO grade and DNA-ploidy. The MIB-1 score was related to T category (P<0.001), WHO grade (P<0.001), DNA ploidy (P<0.0001), M/V index (P<0.0001) and fraction of cells in S phase (P<0.0001). The mean MIB-1 score was 6.37% for G1, 14.59% for G2 and 28.59% for G3 carcinomas (P<0.001). The MIB-1 score for Ta tumors was 9.24% and for T1 tumors 25.34% (P<0.001). The M/V index was 3.9 for G1, 11.5 for G2 and 25.9 for G3 tumors (P<0.0001). The M/V index for Ta tumors was 6.4 and 25.3 for T1 tumors (P<0.0001). WHO grade 1 tumors had 7.7%, grade 2 tumors 13.8% and grade 3 tumors 21.8% of cells in S phase (P<0.001). Of grade 1 tumors, 97% were diploid and 3% aneuploid, and 78% of grade 2 tumors were diploid and 22% aneuploid. Of grade 3 tumors, 30% were diploid and 70% aneuploid (P<0.001). Of Ta tumors, 92% were diploid and 8% aneuploid, respectively, whereas 40% of T1 tumors were diploid and 60% aneuploid (P<0.0001). The results show that quantitative cell proliferation indices are associated with T category and WHO grade in superficial bladder cancer. The prognostic value of the S-phase fraction and mitotic index has been demonstrated in several previous analyses of prognostic factors while the value of MIB-1 score on bladder cancer prognosis remains to be established in further follow-up studies.

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Authors and Affiliations

  1. Department of Surgery, Mikkeli Central Hospital, Porrassalmenkatu 35-37, FIN-50100, Mikkeli, Finland

    T. J. O. Liukkonen

  2. Department of Pathology, University of Kuopio, Kuopio, Finland

    P. K. Lipponen

  3. Department of Pathology, Mikkeli Central Hospital, Mikkeli, Finland

    M. Helle

  4. Department of Pathology, Tampere University Hospital, Tampere, Finland

    H. K. Haapasalo

  5. Department of Pathology, University of Helsinki, Helsinki, Finland

    S. Nordling

  6. Department of Urology, Turku University Hospital, Turku, Finland

    P. Rajala

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  1. T. J. O. Liukkonen
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  4. H. K. Haapasalo
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Liukkonen, T.J.O., Lipponen, P.K., Helle, M. et al. Expression of MIB-1, mitotic index and S-phase fraction as indicators of cell proliferation in suerficial bladder cancer. Urol. Res. 24, 61–66 (1996). https://doi.org/10.1007/BF00296736

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