Summary
The odontoclast, which is morphologically similar to osteoclast, is thought to play a major role in root resorption of deciduous teeth. High collagenolytic activity has been detected in the root resorbing tissue. In order to identify collagenase-producing cells and the role of collagenase in root resorption of deciduous tooth, in situ hybridization of collagenase mRNA in bovine root-resorbing tissue sections was performed using a digoxigenin-labeled, nonradioactive RNA probe. Collagenase mRNA expression was clearly observed in odontoclasts in addition to the macrophages, fibroblasts, odontoclasts, and cementoblasts. Multinuclear odontoclasts showed intense tartrate-resistant acid phosphatase (TRAP) activity. We also examined interleukin-1 (IL-1) mRNA expression in the root-resorbing tissue by in sity hybridization. IL-1 transcripts were found to be expressed in odontoclasts, fibroblasts, and macrophages suggesting that IL-1 might be an important factor for promoting root resorption. These results suggest that the collagenase produced by odontoclasts may play a key role in dentin collagen degradation in root resorption.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Oguchi H (1975) In vitro studies of bone resorption by rootresorbing tissue from the bovine deciduous tooth. Bull Tokyo Med Dent Univ 22:175–183
Morita H, Yamashita H, Shimizu M, Sasaki S (1970) The collagenolytic activity during root resorption of deciduous tooth. Arch Oral Biol 15:503–508
Sakamoto S, Sakamoto M (1988) Degradative processes of connective tissue proteins with special emphasis on collagenolysis and bone resorption. Mol Aspects Med 10:299–428
Sakamoto S, Sakamoto M (1982) Biochemical and immunohistochemical studies on collagenase in resorbing bone in tissue culture. J Periodont Res 17:523–536
Sakamoto M, Sakamoto S (1984) Immunocytochemical localization of collagenase in isolated mouse bone cells. Biomed Res 5:29–38
Sakamoto S, Sakamoto M (1984) Isolation and characterization of collagenase synthesized by mouse bone cells in culture. Biomed Res 5:39–46
Blair HC, Kahn AJ, Crouch EC, Jeffry JJ, Teitelbaum SL (1986) Isolated osteoclasts resorb the organic and inorganic components of bone. J Cell Biol 102:1164–1172
Noorden CJFV, Vogels IMC, Smith RE (1989) Localization and cytophotometric analysis of cathepsin B activity in unfixed and undecalcified cryostat sections of whole rat knee joints. J Histochem Cytochem 37:617–624
Baron R, Eeckhout Y, Neff L, Francois-Gillet C, Henriet P, Delaisse JM, Vaes G (1990) Affinity purified antibodies reveal the presence of (pro)collagenase in the subosteoclastic bone resorbing compartment. J Bone Miner Res 5:s203
Everts V, Delaisse JM, Korper W, Niehof A, Vaes G, Beertsen W (1992) Degradation of collagen in the bone-resorbing compartment underlying the osteoclast involves both cysteine proteinases and matrix metalloproteinases. J Cell Physiol 150:221–231
Nomura S, Wills AJ, Edwards DR, Heath DR, Heath JK, Hogan BLM (1988) Developmental expression of 2ar(osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization. J Cell Biol 106:441–450
Cox KH, Delon DV, Angerer LM, Angerer RC (1984) Detection of mRNA in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol 101:485–502
March CJ, Mosley B, Larsen A, Cerretti DP, Braedt G, Price V, Gillis S, Henney CS, Kronheim SR, Grabstein K, Conlon PJ, Hopp TP, Cosman D (1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature 315:641–647
Burstone MS (1958) Histochemical demonstration of acid phosphatases with naphtol AS-phosphates. J Natl Cancer Inst 21: 523–539
MacNaul KL, Chartrain N, Lark M, Tocci MJ, Hutchinson NI (1990) Discoordinate expression of stromelysin, collagenase, and tissue inhibitor of metalloproteinases-1 in rheumatoid human synovial fibroblasts. J Biol Chem 265:17238–17245
Sasaki T, Watanabe C, Shimizu T, Debari K, Segawa K (1990) Possible role of cementoblasts in the resorbant organ of human deciduous teeth during root resorption. J Periodont Res 25:143–151
Sasaki T, Shimizu T, Watanabe C, Hiyoshi Y (1990) Cellular roles in physiological root resorption of deciduous teeth in the cat. J Dent Res 69:67–74
Hoshino T, Kishi J, Kawai T, Kobayashi K, Hayakawa T (1986) Immunoelectron microscopic localization of collagenase inhibitor in bovine dentin. Collagen Rel Res 6:303–312
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Okamura, T., Shimokawa, H., Takagi, Y. et al. Detection of collagenase mRNA in odontoclasts of bovine root-resorbing tissue by In situ hybridization. Calcif Tissue Int 52, 325–330 (1993). https://doi.org/10.1007/BF00296659
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00296659